Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual page 48

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Problem
High baseline drift
(Cont'd)
High noise level, non-
periodic baseline
fluctuation
Periodic baseline
fluctuation, pulsation
Reproducible ghost peaks
in the chromatogram.
Additional peaks appear
in the injection peak.
Page 42
Probable Cause
The eluents are degraded or
inhomogeneous.
The environmental conditions are
unstable.
The mobile phase is delivered in
circles.
For additional causes, refer to the
operating instructions for the
detector.
The eluent is degraded or of poor
quality/purity.
For additional causes, refer to the
operating instructions for the
detector.
There are pressure fluctuations
from the pump.
There are air bubbles in the system.
The degassing channels are
contaminated.
The eluents are degraded, dirty or
of poor purity/quality.
Contamination occurs somewhere
in the system.
With gradients, the equilibration
time after the flush cycle is too
short.
There is excessive dead volume.
Remedial Action
Before you start an analysis, be
sure that the eluents are already
homogenized in the reservoirs.
Use fresh solvent and check the
eluent filter frits. In aqueous
solvents, growth of
microorganisms is possible.
Make sure that the temperature
and the humidity are constant.
Avoid draft. Verify on the
detector that the lamp and flow
cell covers are in proper
position and that the front panel
door is closed.
Direct the mobile phase to
waste.
→ Detector manual
Use fresh solvents. Use HPLC
grade solvents (MS grade if the
SRD is operated together with
an NCS-3500RS or NCP-
3200RS).
→ Detector manual
Purge the pump
(→ Pump manual).
Purge the pump
(→ Pump manual).
Rinse the degassing channels
(→ page 46).
Use fresh eluent. Use HPLC
grade solvents (MS grade if the
SRD is operated together with
an NCS-3500RS or NCP-
3200RS).
Flush the system using an
appropriate solvent.
Extend the equilibration time.
Eliminate possibly existing
dead volume.
Operating Instructions