Thermo Scientific Orbitrap Fusion Lumos Getting Started Manual page 96

C
Preparing the Reserpine Sample Solution
78 Orbitrap Tribrid Series Getting Started Guide
 To prepare the reserpine sample solution
1. Transfer 900 μL of 1% acetic acid in 50:50 methanol/water into a clean, minimum
1.5 mL polypropylene tube.
2. Add 100 μL of the 100 pg/μL reserpine standard solution to the tube.
The Orbitrap Tribrid Series Chemicals Kit
3. Mix the solution (10 pg/μL) thoroughly.
4. Transfer 100 μL of the 10 pg/μL solution into a clean, minimum 1.5 mL polypropylene
tube.
5. Add 900 μL of 1% acetic acid in 50:50 methanol/water to the tube.
6. Mix the solution (1 pg/μL) thoroughly.
7. Transfer 50 μL of the 1 pg/μL solution into a clean, minimum 1.5 mL polypropylene
tube.
8. Add 950 μL of 1% acetic acid in 50:50 methanol/water to the tube.
9. Mix the solution thoroughly.
10. Label the tube Reserpine Sample Solution (50 fg/μL).
contains the reserpine standard solution.
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