Thermo Scientific Orbitrap Fusion Lumos Getting Started Manual page 23

Table 2. Guidelines for setting operating parameters for LC/H-ESI/MS
LC flow Spray
rate (μL/min) voltage (V)
Up to 15 Pos: 3500
Neg: –2500
16–99 Pos: 3500
Neg: –2500
100–199 Pos: 3500
Neg: –2500
200–500 Pos: 3500
Neg: –2500
a
Positive and negative polarity modes
Table 3. Guidelines for setting operating parameters for LC/APCI/MS
LC flow rate
(μL/min)
Sheath
Up to 5 15
5–199 25
200–500 45
a
Positive and negative polarity modes
Table 4. Guidelines for setting operating parameters for LC/NSI/MS
Spray voltage (V)
Positive mode: 1200
Negative mode: –600
Thermo Scientific
Gas (arbitrary units)
a
Sheath Auxiliary
5 2
25 5
35 7
50 10
Gas (arbitrary units)
Auxiliary Sweep
5
5
5
Sweep gas (arbitrary units)
2
Ion transfer tube
temp (°C)
Sweep
Fusion MS: 275
0
Lumos MS: 325
Fusion MS: 275
0
Lumos MS: 325
Fusion MS: 300
0
Lumos MS: 325
1 325
Ion transfer tube
temp (°C)
Fusion MS: 250
0
Lumos MS: 300
Fusion MS: 250
0
Lumos MS: 300
Fusion MS: 275
0
Lumos MS: 325
LC Flow Rate Ranges
Vaporizer Typical nitrogen
temp (°C) gas consumption
20
75
200–275
275–350
Vaporizer Corona
temp (°C) discharge
current (μA)
275 Pos: 4
Neg: –10
325 Pos: 4
Neg: –10
350 Pos: 4
Neg: –10
Ion transfer tube temperature (°C)
275
Orbitrap Tribrid Series Getting Started Guide
1
Introduction
(L/min)
3.5
8.0
10.5
15.0
a
5