Thermo Scientific Orbitrap Fusion Lumos Getting Started Manual page 43

 To set up the inlet for manual-loop injection (H-ESI or APCI mode)
Connect a sample loop across ports 1
and 4.
Connect a loop filler (needle port) to
port 2.
Using a Rheodyne fitting, connect one end of the Teflon tubing to
port 3. Place the other end into an appropriate waste container.
Using red PEEK tubing, connect port 5 (with a one-piece fingertight
fitting) to the LC pump outlet (with an appropriate fitting and
ferrule).
(H-ESI mode) Using two pieces of red PEEK tubing, make these connections:
• Connect port 6 (with a one-piece fingertight fitting) to the installed
grounding union (with a two-piece fingertight fitting).
• Connect the other end of the grounding union (with a two-piece fingertight
fitting) to the H-ESI spray insert's sample inlet (with a one-piece fingertight
fitting).
(APCI mode) Using red PEEK tubing, connect port 6 (with a one-piece
fingertight fitting) to the APCI spray insert's sample inlet (with a two-piece
fingertight fitting).
(Optional, not shown) If you installed the H-ESI plumbing path, you can
connect the tubing to the installed grounding union.
Note For APCI mode, you do not need to remove the grounding bar if you
choose not to use the H-ESI plumbing path.
6
(Not shown) Load a clean syringe with the sample solution, carefully insert it into the
needle port that is installed in port 2, and then slowly press the syringe plunger.
Thermo Scientific
Setting Up the Inlet Plumbing for the Sample Introduction Techniques
1
2
3
3
4
5
5
Orbitrap Tribrid Series Getting Started Guide
Connecting the Inlet Plumbing
1
3 6
4 5
Loop injector valve
H-ESI mode
APCI mode
25