Thermo Scientific Orbitrap Fusion Lumos Getting Started Manual page 63

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5. Click the arrow next to the Positive check box, and then select the Ion Optics check box.
6. Click Start and review the real-time plot of the mass spectrum.
After the Tune parameters reach their specified settings, the calibration process begins and
the status area appears in the Calibration pane. After completing the calibration, the Tune
application adds a change record to the History pane under History Logs.
7. Run the calibration for each of the remaining Positive and Positive Extended categories
(as applicable for your MS)—one at a time and in the order specified in the Tune
window.
IMPORTANT The Predictive AGC calibration depends on the other calibrations.
Therefore, you must run this as the last positive calibration.
Tip After the calibration, you see either a green check (
name to indicate a successful calibration or a red X mark (
calibration.
A date appears in the Last Calibrated column for each successful calibration test. A date
does not appear for failed calibrations.
8. If using the traditional calibration solution, do the following:
a. Follow the procedure in
b. Load the syringe with the negative calibration solution.
c. Run the Negative calibrations one at a time and in the order specified in the Tune
window.
9. If using the FlexMix calibration solution, run the Negative calibrations one at a time and
in the order specified in the Tune window.
IMPORTANT Before you start using your analyte, follow the procedure in
Inlet Components After
6
Calibrating the Mass Spectrometer in H-ESI Mode
Flushing the Inlet Components After
Calibration.
Running the Calibration Procedures
) adjacent to the calibration
) to indicate a failed
Calibration.
Flushing the
Orbitrap Tribrid Series Getting Started Guide 45