Thermo Scientific Orbitrap Fusion Lumos Getting Started Manual page 8

Contents
Chapter 4
Chapter 5
Chapter 6
Chapter 7
Chapter 8
Appendix A Using Basic Tune Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61
viii Orbitrap Tribrid Series Getting Started Guide
Setting Up the Inlet Plumbing for the Sample Introduction Techniques . . . . . . 21
Setting Up the Inlet for Direct Infusion . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Setting Up the Inlet for HPLC with an Autosampler. . . . . . . . . . . . . . . . . . . 22
Setting Up the Inlet for High-Flow Infusion Without an Autosampler . . . . . 23
Setting Up the Inlet for Loop Injections (Flow-Injection Analysis) . . . . . . . . 24
Connecting the Grounding Union to the H-ESI Spray Insert . . . . . . . . . . . . . . 26
Preparing the System for Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
Setting Up the Syringe Pump for Direct Infusion . . . . . . . . . . . . . . . . . . . . . . . 27
Setting Up the Mass Spectrometer for Calibration. . . . . . . . . . . . . . . . . . . . . . . 28
Establishing a Stable Ionization Spray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31
Evaluating the Spray Stability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Optimizing the Ion Source Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Calibrating the Mass Spectrometer in H-ESI Mode . . . . . . . . . . . . . . . . . . . . . . . .37
Calibration Solution Peak Values . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Peak Values for the Traditional Calibration Solutions . . . . . . . . . . . . . . . . . . 38
Peak Values for the Pierce FlexMix Calibration Solution . . . . . . . . . . . . . . . . 40
Running the Calibration Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Using Tune to Acquire Sample Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47
Setting Up the LC/MS System for Analyte Optimization . . . . . . . . . . . . . . . . . 47
Setting Up the Inlet. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Configuring the Syringe Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Configuring the LC Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Configuring the Ion Source for Reserpine (Example). . . . . . . . . . . . . . . . . . . 49
Setting the Instrument's Optimal Pressure. . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Defining the Scan Parameters for Precursor Optimization. . . . . . . . . . . . . . . . . 50
Defining the MS/MS Scan Parameters for Precursor Optimization . . . . . . . . 50
Optimizing the Isolation Window. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Optimizing the Ion Source Parameters on an Analyte . . . . . . . . . . . . . . . . . . 53
Optimizing the Fragmentation Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Defining the Scan Parameters for an MS
Acquiring a Data File by Using the Tune Application . . . . . . . . . . . . . . . . . . 55
Using the Xcalibur Data System to Acquire Data . . . . . . . . . . . . . . . . . . . . . . . . . .57
Selecting the External Start Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Acquiring a Data File by Using the Xcalibur Data System . . . . . . . . . . . . . . . . . 59
Opening the Tune Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Setting the Instrument System Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Setting the Instrument Power Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Checking the Instrument Readback Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
3
Scan . . . . . . . . . . . . . . . . . . . . . . . . . 54
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