Thermo Scientific Orbitrap Fusion Lumos Getting Started Manual page 11

Figures
Figure 1.
Figure 2.
Figure 3.
Figure 4.
Figure 5.
Figure 6.
Figure 7.
Figure 8.
Figure 9.
Figure 10.
Figure 11.
Figure 12.
Figure 13.
Figure 14.
Figure 15.
Figure 16.
Figure 17.
Figure 18.
Figure 19.
Figure 20.
Figure 21.
Figure 22.
Figure 23.
Figure 24.
Thermo Scientific
MS ion source mount assembly and ion sweep cone . . . . . . . . . . . . . . . . . . . . . 11
Proper connection for the PEEK tubing and fitting (syringe adapter
assembly) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Schematics of the sample introduction techniques (examples) . . . . . . . . . . . . . . 20
Plumbing connections for the grounding union (H-ESI mode) . . . . . . . . . . . . . 26
Syringe pump settings box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Plot Chromatogram dialog box with the TIC option selected . . . . . . . . . . . . . . 32
Optimization page of the Ion Source pane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Report Generation Options dialog box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Traditional n-Butylamine calibration solution's normal mass-range spectrum
in positive ion polarity mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Traditional Ultramark 1621 calibration solution's normal mass-range
spectrum in negative ion polarity mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
FlexMix calibration solution's low-mass range spectrum in positive ion
polarity mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
FlexMix calibration solution's low-mass range spectrum in negative ion
polarity mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
FlexMix solution's normal-mass range spectrum in positive ion polarity
mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
FlexMix solution's normal-mass range spectrum in negative ion polarity
mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
FlexMix solution's high-mass range spectrum in positive ion polarity
mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
FlexMix solution's high-mass range spectrum in negative ion polarity
mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Calibration pane (example for Orbitrap Fusion and Orbitrap Fusion Lumos
MSs) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Syringe pump settings box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
CID-MS/MS scan spectrum with fragmentation (reserpine example) . . . . . . . . 52
3
CID-MS scan spectrum with fragmentation (reserpine example). . . . . . . . . . . 55
Data Acquisition pane in the Tune window . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Run Sequence dialog box (partial) showing the selected start instrument . . . . . 58
Change Instruments In Use dialog box showing the MS as the start
instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Orbitrap Fusion Lumos Tune window showing the Define Scan pane . . . . . . . 62
Orbitrap Tribrid Series Getting Started Guide
F
xi