Thermo Scientific Orbitrap Fusion Lumos Getting Started Manual page 28

2
Setting Up the Ion Source
Preparing the Mass Spectrometer
10 Orbitrap Tribrid Series Getting Started Guide
 To prepare the mass spectrometer
1. Complete all data acquisition, as applicable.
2. In the Tune window, place the MS in Off mode
The LC/MS system is now in off mode (see Chapter 6 in the Hardware Manual for a list
of components that remain on). After the ion source housing and spray insert have cooled
to room temperature, you can safely remove these components.
Note Always place the system in off mode before removing the spray insert or the ion
source housing.
3. If you want to change the installed source, wait until it has cooled to room temperature.
For instructions on how to remove the source, refer to its manual.
CAUTION Hot surface. Avoid touching the ion source housing when the MS is in
operation. The external surface of the housing can become hot enough to burn your
skin. Allow the housing to cool before you touch it.
4. Depending on the ionization mode, do the following
• For H-ESI, APCI, or APPI mode, install the ion sweep cone over the MS's spray cone
(Figure 1).
• For NSI mode, loosen the screws on the ion sweep cone, and then remove the cone
by grasping its outer ridges and pulling it off.
(Table 9).
(Figure 1):
Thermo Scientific