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Related Manuals for NuAire NU-5510
Summary of Contents for NuAire NU-5510
- Page 1 Air-Jacketed Automatic CO Incubator Models NU-5510 NU-5510E Operation and Maintenance Manual October, 2010 Revision 12 (Series 6 & Higher) Manufactured By: NuAire, Inc. 2100 Fernbrook Lane Plymouth, MN 55447 Toll-Free: 1-800-328-3352 In Minnesota: (763)-553-1270 Fax: (763)-553-0459 OM0135...
- Page 2 10.2................Chemical Decontamination Section No. 11..............Error Indicators & Troubleshooting Section No. 12..............Remote Alarm Contacts Section No. 13..............Electrical/Environmental Requirements Insert..................Replacement Parts List MANUAL DRAWINGS BCD-09807 ..........NU-5510/E Direct Heat CO Incubator BCD-09409 ..........Plenum/Shelf Installation BCD-09709 ..........Front Control Panel BCD-09809 ..........Rear Panel ASSEMBLY DRAWINGS BCD-09810 ..........Front Panel Assembly...
- Page 3 Sterility Reliability Like all NuAire equipment, this Incubator has been designed to provide the highest quality standards of performance with matching computer technology, precise temperature control, and CO gas control system combining state-of-the-art technology with years of design, quality, and manufacturing experience.
- Page 4 Incubator CO Control The NuAire direct heat Incubator incorporates a microprocessor-based, non-dispersive infrared CO induction sensor. The amount of energy received at the detector is an approximate logarithmic function of the CO concentration in the gas between source and detector. The wavelengths used are absorbed only by making the measurement insensitive to other components, such as water vapor.
- Page 5 Performance Parameters and Features Both the interior and exterior of the DHD Autoflow are constructed of 16-gauge material. The interior is highly polished type 304 stainless steel, using crevice-free construction. All exposed edges are deburred to insure no sharp edges. The exterior is cold rolled steel finished in a powder coated polyurethane finish, which is resistant to chemicals and easily cleaned using mild household detergents.
- Page 6 Models & Features NuAire offers a basic Model NU-5510/E Air-Jacketed DHD Autoflow CO Incubator. Dimensions (see also Specification Drawing BCD-09807) Overall Dimensions - inches (mm): Model NU-5510/E Height: Exterior: 39.5 Inches (1003.3mm) Width: 25.5 Inches (647.7mm) Depth: 26.5 Inches (673.1mm)
- Page 7 Standard Items Packed With Unit Four (4) stainless steel shelves Eight (8) stainless steel shelf brackets Two (2) sidewall plenums (installed) One (1) blower plenum (installed) One (1) water pan Gas tube and filter ...
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- Page 9 Test Performance & Procedures All equipment is thoroughly inspected at the NuAire Factory at the time of shipment. Quality control is maintained by constant surveillance over the product, beginning at the receipt of purchased material and concluding with a final inspection, which certifies the Incubator performance to the specifications. In all instances where product quality cannot be easily assessed on the end item, the product is inspected during sub-assembly fabrication.
- Page 10 Claims under this warranty should be directed to NuAire, Inc. setting forth in detail the nature of the defect, the date of the initial installation and the serial and model number of the equipment.
- Page 11 Installation The Incubator is fastened to the base skid and it is usually the best procedure to leave the skid attached until the Incubator is located in its approximate position to facilitate ease in handling. The base skid can then be removed by removing the four bolts holding the cabinet to the skid. Examine the Incubator carefully.
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- Page 13 Shelf Plenums & Water Pan Installation Before installation of the shelves, and water pan, NuAire recommends to decontaminate all surfaces within the interior chamber, glass door, and outer door with gasket. They can be wiped down with a disinfectant of 70 percent alcohol or similar non-corrosive antimicrobial agent. Use only disinfectants that are compatible with the vinyl gasket and the powder coat paint on the outer door.
- Page 14 Air Inlet Connection An air inlet tubing kit consists of one-foot clear vinyl tube and (1) 50mm polypropylene .3-micron HEPA filter. Locate the air inlet port on the back panel of the unit. Remove the cover cap; connect one end of tubing to the air inlet port, and the other end of tubing to the air filter.
- Page 15 CO pressure to the Incubator. Some single stage CO pressure regulators have two gauges. USE A TWO-STAGE REGULATOR. All NuAire Incubators use CO in such small quantities that precise metering of CO input pressure is important for maximum performance.
- Page 16 Air Pump Assembly Shipping Foam: One or more blocks of foam is used to secure the air pump during shipping. IMPORTANT: FOAM BLOCK MUST BE REMOVED BEFORE OPERATION To remove foam block: Remove control center cover by unscrewing the (4) screws holding it to the top of the Incubator.
- Page 17 DHD Autoflow Operation ATTENTION ACCOMPANY'S POTENTIAL ELECTRICAL HOT SURFACE INFORMATION OR IMPORTANT HAZARD ONLY QUALIFIED BURN POTENTIAL SYMBOL PERSON TO ACCESS CAUTION: A qualified technician who is familiar with the proper maintenance procedures required for this equipment, as well as repair must perform all maintenance actions on this equipment. The Incubator is designed to provide a sterile, constant temperature, constant CO level and naturally humidified atmosphere for optimum growth of tissue cell cultures and other organisms requiring this...
- Page 18 EXCEPT WHERE INSTRUCTED TO DO SO. THIS IS A PRESSURE RELIEF FOR THE DECON CYCLES. Control System The NuAire Incubator Control Electronics system is designed to serve the control requirements of the Incubator chamber. Temperature and CO levels are controlled by preset values to provide the optimum conditions for culture growth within a chamber.
- Page 19 The Incubator is in a “standby” condition and heat or gas functions are not active. NUAIRE Logo: When pressed for 3 seconds it gives access to the “tSt”, “Opt” & master reset menus.
- Page 20 NOTE: See Section 11 for all alarm conditions. 8.3.5 Password Protection If desired, the NuAire Incubator Control Electronics may be configured to prevent users who are unauthorized to change the set points (CO %, Temp), running conditions of the Incubator or initiate a Decon Cycle.
- Page 21 8.4.4 Air Class ISO 5 LED Green LED lights and stays on when the chamber air has run through the HEPA filtration system long enough to clean it to ISO Class 5 (Equivalent to US Federal Standard 209E Class 100) air quality.
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- Page 23 Rear Panel Detail The rear control panel contains the following functions (BCD-09809). 8.5.1 Power Cord The power cord is 8-foot (2m) in length, type “SVT” molded plug, allowing for long life and easy cleanability. 8.5.2 Circuit Breaker All control electronics are protected with a circuit breaker that may trip at 110% of load rating, but will trip at 145% of load rating in less than 2 seconds.
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- Page 25 Run Mode Operator Interactions The mode switch is used to select the mode of chamber operation: setup or run. Pressing the run/setup key for three seconds changes the chamber operation mode. The mode switch LED will display a green light when in run mode. In general, there is no need for operator interaction in "run"...
- Page 26 To initiate the diagnostic mode, perform the following: In either run or setup mode, press and hold the hidden key (flag on NuAire logo) for four seconds. Temperature display will indicate the first menu "test". To advance to the second menu, press ▲ key, temp. Display will indicate, "opt".
- Page 27 Once you have made your menu selection, you will remain within that menu selection until you exit the diagnostic mode. If another menu selection is desired, you must re-enter the diagnostic mode via the hidden key. To exit the function parameters, press the hidden key (flag on NuAire logo). 1 - Display/LED Test...
- Page 28 7 - CO Tank 2 Valve This function shows the current state of the CO tank 2 valve. The CO percent display will show "on" or "off" corresponding to the valve condition. 8 - Door Heater This function shows the current state of the door heater. This function also allows the door heater to be turned on at different percentages (0, 25, 50, 75, 100) alternating with the "dor"...
- Page 29 Once you have made your menu selection, you will remain within that menu selection until you exit the diagnostic mode. If another menu selection is desired, you must re-enter the diagnostic mode via the hidden key. To exit the function parameters, press the hidden key (flag on NuAire logo). 1 - CO...
- Page 30 2 - CO Tank 2 Enable (Optional) This function will enable or disable the optional CO tank 2 system. The value display will show "on" or "off" corresponding to the current condition. 3 - CO Tank Switch Back (option) Note: function can only be enabled with the CO tank 2 option in use.
- Page 31 7 - Password Protection This function allows users to disable/enable password to prevent unauthorized change of set point, using the UP, DOWN and SELECT keys combination. Password requires three digits. If password option is enable, whenever 'SET UP' key is pressed, password will be required. Every time password option is disabled and re-enable, old password is cleared and new password will be required.
- Page 32 12 - Air Inject Cycle Time This value specifies the amount of time in minutes between each injection of air into the chamber 13 - Print Frequency Time This parameter specifies the frequency, in minutes that lines are to be printed on a status report. If the frequency is specified as zero, no report will be printed.
- Page 33 18 - Temperature Maximum Above Set point This value determines the maximum deviation, measured in (C) that the chamber is permitted to rise above set point, once it is researched before an alarm condition is declared. An alarm condition will cause the chamber to enter a safe condition where no power is enabled to any of the system output controls until the situation is rectified.
- Page 34 Calibration Proper calibration of the DHD Autoflow involves four parameters: chamber temperature, door temperature, perimeter temperature, and CO sensor. The first three, chamber, door, and perimeter temperature should be completed and stabilized before any CO sensor calibration is performed. Below, each calibration procedure is described in detail.
- Page 35 Door and Perimeter Temperature Calibration The DHD Autoflow's inner glass door and outer shell perimeter temperature calibration is best accomplished by running the Incubator 24 hours with the water pan in place and perform the following calibration sequence, if required. Open the Incubator door and look for general condensation. Some condensation on the glass door can be desirable as an indication of adequate humidity in the chamber.
- Page 36 Setting Air Injections If there is still some undesired condensation in the chamber when the door and perimeter heaters are set for the desired result, the air injections can be adjusted. There is a control for length of the air injection labeled, Air Inject Time, and the frequency that air is injected called, Air Inject Cycle.
- Page 37 9.4.2 Sensor Calibration (Zero/Span/CAL. INJ.) There are 2 sensor (zero/span) calibration routines available to the lab professional. The first option is the “open door” routine involving opening the outer and inner door to zero the sensor. This routine also automatically calibrates to the CO injection rate during the injection for the span portion of the sensor calibration.
- Page 38 CLOSED DOOR SENSOR CALIBRATION ROUTINE (Default “OFF” see section 8.8.1.2 item 4): Activating the routine Press logo key until tSt flashes in display then press ▲ to select the OPt menu. Press "SEL" until C.SC appears in the display. ...
- Page 39 9.4.3 Injection Calibration The CO injection calibration can be performed separately from zero/span calibration to optimize the gas injection time required to recover the CO level to set point after a door opening. The recovery time should be as minimal as possible with virtually no overshoot. Verify the CO sensor calibration prior to performing an injection calibration.
- Page 40 10.0 Maintaining Your DHD Autoflow DHD Autoflow Chamber The chamber maintenance is up to the discretion of the owner and the extent of cleanliness and sterility desired. The shelves and bracket supports are all removable and autoclavable. The interior should be wiped down with an appropriate disinfectant such as 70% ISOPROPYL ALCOHOL or equivalent.
- Page 41 THE HEATED DECONTAMINATION CYCLES Nuaire gives the lab professional the choice of the 2 most commonly accepted, heated decontamination cycles available. They are a 95 C humidified cycle and a 145 C dry cycle. To choose the user goes to options menu and pushes "SEL"...
- Page 42 STARTING THE CYCLE DRY 145 DEG C DECON CYCLE TIME (HRS:MIN:SEC) Press the 95/145 button (for 3 seconds) until the Incubator displays show the following: TEMP. display temperature continuous CO2 display H2O/out flashing The LED over the 95/145 button will flash on /off. Indicates that user should: ...
- Page 43 COOL DOWN & STABILIZING 4 hours When the decontamination period is complete the heaters are shut off and the air pump is turned on blowing HEPA filtered air to cool the chamber to the user chosen temperature set point. ...
- Page 44 USING THE 95 DECON CYCLE The 95º C decontamination cycle is humidified to ensure that the contaminating agents are eradicated. The complete cycle takes about 14 hours to run. (See graph for cycle phases). There is no need to recalibrate or remove the CO IR sensor since it is remote from the chamber.
- Page 45 HUMIDIFIED 95 DEG. C DECON CYCLE TEMPERATURE RELATIVE HUMIDITY Drying Phase Decon Phase TIME (HR:MN:SEC) STARTING THE CYCLE Press the 95/145 button (for 3 seconds) until the Incubator displays show the following: TEMP. display flashing display flashing ...
- Page 46 HEAT UP .75 hours Press the 95/145 button again to start the heat up portion of the cycle. Temperature display shows chamber temperature. display flashes dCn The LED over the 95/145 button will flash on /off. DECONTAMINATION 9 hours ...
- Page 47 10.2 Chemical Decontamination of the Incubator Chamber To chemically decontaminate NuAire Incubators, users may use the traditional formaldehyde, Vapor Phased Hydrogen Peroxide, or Chlorine Dioxide. All three of the chemicals are compatible to all parts within NuAire Incubators. NOTE: As stated previously, the chamber and components can also be wiped down with a 70% solution of Isopropyl Alcohol for cleaning and decontamination.
- Page 48 2. Replace fuse. -Temperature time out error during 3. Faulty TRIAC, replace control board normal running and in Decon Cycle 4. Faulty chamber heater contact NuAire Technical Service. 5. Door/Perimeter heater needs to be - Decon Time Out increased with a high temperature...
- Page 49 - Cal inject calibration failed. Not 1. Check gas supply then run calibration again enough increase in the CO reading 2. Call NuAire Technical Services if error after gas was injected. persists. 1. Zero span calibrate the CO sensor - Auto zero failure.
- Page 50 2. Remove top plenum and ensure that blower EXCESS VIBRATION wheel is not rubbing on anything. 3. Check jacket fan mounted in bottom on Incubator For further assistance, call NuAire Customer Service at 1-800-328-3352 or (763) 553-1270 USA. OM0135...
- Page 51 Remote Alarm Contacts The NuAire DHD Autoflow contains a set of contact points to connect to a remote alarm system. The contacts are located on the rear panel. The contacts are housed in a modular (RJ-11) telephone jack and rated for (30V at 1 Amp).
- Page 52 13.0 Electrical/Environmental Requirements 13.1 Electrical NU-5510 115V, 50/60Hz, 1 Phase, 12.5 Amps NU-5510E 230V, 50/60Hz, 1 Phase, 5 Amps Start Up Power 345 WATTS Running Power 175 WATTS Decon Cycle 1190 WATTS 13.2 Operational Performance (for indoor use only) Environment Temperature Range: 60F-85F (15C - 30C)
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