NuAire 12 Series Operation And Maintenance Manual
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NuAire 12 Series Operation And Maintenance Manual

Air-jacketed dh invitrocell co2 incubator
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Air-Jacketed DH Invitrocell CO
Incubator
2
Models
NU-5800 (Series 12)
NU-5800E (Series 12)
Operation and Maintenance Manual
March 2022
Revision 6
115V 50/60Hz ONLY
Manufactured By:
NuAire, Inc.
2100 Fernbrook Lane
Plymouth, MN 55447
Toll-Free: 1-800-328-3352
In Minnesota: (763)-553-1270
Fax: (763)-553-0459
OM0245
Page 1 of 53

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Summary of Contents for NuAire 12 Series

  • Page 1 Models NU-5800 (Series 12) NU-5800E (Series 12) Operation and Maintenance Manual March 2022 Revision 6 115V 50/60Hz ONLY Manufactured By: NuAire, Inc. 2100 Fernbrook Lane Plymouth, MN 55447 Toll-Free: 1-800-328-3352 In Minnesota: (763)-553-1270 Fax: (763)-553-0459 OM0245 Page 1 of 53...

  • Page 2: Table Of Contents

    Air-Jacketed DH Invitrocell CO Incubator Operation & Maintenance Manual NU-5800/NU-5800E Table of Contents Section No. 1 ..........General Description Section No. 2 ..........Performance Parameters and Features Section No. 3 ..........Models & Features Section No. 4 ..........Test Performance & Procedures Section No. 5 ..........Warranty Disclaimer Section No.

  • Page 3: Incubator

    Sterility Reliability Like all NuAire equipment, this Incubator has been designed to provide the highest quality standards of performance with matching computer technology, precise temperature control, and CO gas control system combining state-of-the-art technology with years of design, quality, and manufacturing experience.
  • Page 4 Keep these operating instructions close to the unit so that safety instructions and important information are always accessible. • Should you encounter problems that are not detailed adequately in the operating instructions, please contact your NuAire Representative of NuAire technical Services. OM0245 Page 4 of 53 Rev 6 March/2022...
  • Page 5 1.9 Explanation of symbols Symbol Description Safety alert symbol indicates a potentially hazardous WARNING situation which, if not avoided, could result in death of serious injury. Safety alert symbol indicates a potentially hazardous CAUTION situation which, if not avoided, may result in minor or moderate injury.
  • Page 6 Performance Parameters and Features • Both the interior and exterior of the DH are constructed of 18 to 16-gauge steel. The interior is highly polished type 304 L 16 gauge stainless steel, using crevice-free construction. All exposed edges are deburred to insure no sharp edges. The exterior is cold rolled 16 &...
  • Page 7 Models & Features 3.1 Dimensions (see also Specification Drawing BCD-18314) Overall Dimensions - inches (mm): Model NU-5800/E Height: Exterior: 39.688 in (1008 mm) Width: 26.750 in (680 mm) Depth: 27.188 in (691 mm) Foot print: 23.5 in (597 mm) wide x 20.75 in (527 mm) deep Height: Interior: 28.500 in (723.900mm) Width:...
  • Page 8 3.3 Standard Items Packed With Unit Four (4) stainless steel shelves • • Eight (8) stainless steel shelf brackets One (1) water pan • • Gas supply tube(s) • Access port plug - vented One (1), 2 meter (6.5 ft.) electrical cord 10 Amp 18awg with 70°C rated jacket * •...
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  • Page 10 Test Performance & Procedures All equipment is thoroughly inspected at the NuAire Factory at the time of shipment. Quality control is maintained by constant surveillance over the product, beginning at the receipt of purchased material and concluding with a final inspection, which certifies the Incubator performance to the specifications.
  • Page 11 However, damage can occur in any shipment and the following paragraph outlines those steps you should take on receipt of a NuAire Incubator to be sure that if damage has occurred, the proper claims and actions are taken immediately.
  • Page 12 7.3 Interior Chamber, Shelf & Water Pan preparation. Chamber & Shelves Incubator is shipped with the shelves installed. A shelf mounted water pan is also available. NuAire recommends decontaminating all surfaces within the interior chamber, glass door, and outer door with gasket. They can be wiped down with a disinfectant of 70 percent alcohol or a similar non-corrosive antimicrobial agent.
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  • Page 14 7.4 Electrical The electrical supply circuit to the Incubator must conform to all national and local electrical codes. Consult the serial-data plate, located at the front of the right side of the Incubator, for voltage, cycle, phase, and ampere requirements before making connection. Plug the power cord securely into a grounded power source. VOLTAGE SHOULD NOT VARY MORE THAN 5% FROM SERIAL PLATE RATINGS.
  • Page 15 0 to 2 BAR (100 PSI or 6 BARS maximum). This gauge will indicate the actual CO pressure to the Incubator. Some single stage CO pressure regulators have two gauges. USE A TWO-STAGE REGULATOR. All NuAire Incubators use in such small quantities that precise metering of CO input pressure is important for maximum performance.
  • Page 16 7.7 Remove the cable tie used to secure the air pump for shipping: 7.8 Setting up the access port: When utilizing the access port do not block or restrict the relief holes in the center of the port plug. Blocking this venting can cause performance issues for the incubator.
  • Page 17 Use mild detergent to clean the exterior of the Incubator. NOTE: NuAire does not offer any product warranty with respect to cleaning material compatibility. USE AT YOUR OWN RISK! The information provided above is from raw material suppliers and known general source documents for use to develop application cleaning SOP’s.

  • Page 18: Control System Introduction

    8.3 Control System Introduction The NuAire Incubator Control Electronics system is designed to serve the control requirements of the Incubator chamber. Temperature and CO levels are controlled by preset values to provide the optimum conditions for culture growth within a chamber.
  • Page 19 NU-Touch LCD menu system is accomplished through the following methods: • Touching a “graphic” Icon like the NuAire Logo in the Main Screen which access the System Settings menu, or lead you to an Enter Password screen when a password has been established.
  • Page 20 8.3.5 Functioning accessed from the System Settings screen: The “System Settings” menu screen can be accessed from the “Main Screen” by touching the NUAIRE logo: Environmental Set-Points: Allows adjustment of Temperature, CO2, Door, and Perimeter heater power level settings. Note: You can also access the Environmental set points by touching any control system numerical value in the MAIN SCREEN.
  • Page 21 A USB method of updating the software 8.3.9 Diagnostic and calibration assists. To access the Service Settings Menu, perform the following steps: NuAire Logo → Service Settings → 9876 Enter. You are now in the Service Settings Menu (screen). This screen provides you the capability to review and analyze numerous Incubator functions including the following: •...
  • Page 22 The “Alarm Status” button indicates is triggered when a timer for a maintenance reminder runs to zero. This text appears in red just below the NuAire Logo (paragraphs 8.4.2 and 8.4.3). The “Alarm Status” button and audible alarm indicates the abnormality. A catastrophic temperature control condition will de-energize the safety relay and cause the chamber to cool below the set point.
  • Page 23 8.5 Rear Panel Detail The rear control panel contains the following functions (BDC-15669) 8.5.1 Power Cord The power cord is 6.5-foot (2 m) in length, type “SVT” molded plug, allowing for long life and easy cleaning. 8.5.2 Circuit Breaker All control electronics are protected with a circuit breaker that may trip at 110% of load rating but will trip at 145% of load rating in less than 2 seconds.
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  • Page 25: Front Component Panel

    8.6 Front Component Panel The front component panel contains the following functions. See 18316 8.6.1 CO2 Supply Filter The CO2 supply filter is installed at the factory and should be changed following the instructions found in Maintenance Section 10. 8.6.2 Air Inlet Filter - All models The Air Inlet Filter is installed at the factory and should be changed following the instructions found in Maintenance section 10.
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  • Page 27: Run Mode Operator Interactions

    8.8 Standby Mode Operator Interactions The NuAire incubator automatically enters the standby mode when necessary. There is no action required by the user to enter this mode. All functions that put the incubator in standby mode automatically start a 1-minute timer. If the function is not interfaced through the screen on the NU-Touch display the incubator then returns to the MAIN SCREEN and switches to RUN mode.
  • Page 28 The diagnostic mode allows the operator to configure and/or check the Incubator for input/output signals manually and individually. To initiate the diagnostic mode, perform the following from the ‘Main’ screen: Select the NuAire Logo to enter “System Settings” • •...
  • Page 29 Output/Input Diagnostic Parameter Functions Temperature 1. Safety Relay (on/off) 2. Chamber Temp. Sensor (shows current temp.) 3. Safety Temp. Sensor (shows current temp.) 4. Chamber heater Triac control (0 - 50% - 100%) 5. Door Heater Triac control (0 - 50% - 100%) 6.
  • Page 30 5. Door Heater This function shows on LCD the current state of the door heater. This function also allows the door heater to be turned on at different percentages (0, 50, and 100 percent). The LCD display will show phase firing percentage chosen default is 0%.
  • Page 31 8.10 Option Configuration Parameters Format: Option (Default, Minimum/Maximum) All the options for the NU-5800 Incubator use this same format. The default is set at the factory and can be restored in the field with a Master or a Factory reset command. On the following pages, there is a short description of each Option with its Default/Minimum/Maximum setting, followed by a more detailed description.
  • Page 32 Screen Lock button (default Main Screen Unlocked). Pressing this button will change it to “Main screen Locked” (Lockout = 1). This puts a “Press to unlock” button on the Nuaire Logo in the Main screen. This feature stops accidental screen changes by brushing the touch screen. You must press and hold (2 seconds) until the message disappears which unlocks the main screen so that you can navigate into;...

  • Page 33: Resetting The Electronic Software

    “Press to Unlock” button from the Nuaire Logo and the Main screen goes back to the default condition. • Power Loss Mode cancel button. Cancels Power loss mode. Only use this if it is certain that there is no condensation in the circulation system. See Section 10.2 for further details about the Power Loss Mode.
  • Page 34 Setting the model number sets the following function: All NuAire customers will press NuAire in the “Choose Company” Screen. This will bring up the • NuAire logo in the main screen. • Model NU-5800 and NU-5800E or NU-5800D Enables Temperature and CO2 the only control systems available on this model Chooses the algorithm suited for low watt heating of the chamber Supresses the decon button in the “Systems Settings”...

  • Page 35: Chamber Temperature Calibration

    Calibration Proper calibration of the incubator involves four parameters: chamber temperature, door temperature, perimeter temperature, and CO sensor. The first three, chamber, door, and perimeter temperature should be completed and stabilized before any CO sensor calibration is performed. Below, each calibration procedure is described in detail. For the best results, follow the procedure carefully, and if the desired result is not achieved, try procedure again from the start.
  • Page 36 The inner glass door and outer shell perimeter temperature calibration is best accomplished by running the Incubator for a minimum of 24 hours with the water pan in place and perform the following calibration sequence, if required. Open the outer Incubator door and look for general condensation. Some condensation on the glass door can be desirable as an indication of adequate humidity in the chamber.
  • Page 37 9.4 CO Calibration The DH infrared CO sensor may be calibrated using the following technique: Open Door Zero-Span calibration, CO2 calibrations can be performed in approximately 2 minutes, by accessing the CO2 calibrations menu in “Service Settings” and following the on-screen instructions. To perform open door CO2 calibration routine Enter CO2 calibrations by means of Service Settings •...

  • Page 38: Chemical Decontamination

    SUCH MATERIAL IS HARMFUL TO THE POLISHED STAINLESS STEEL. NOTE: NuAire does not offer any product warranty with respect to cleaning material compatibility. USE AT YOUR OWN RISK! The information provided above is from raw material suppliers and known general source documents for use to develop application cleaning SOP’s.

  • Page 39: Power Loss Recovery Mode

    10.2 Power Loss Recovery Mode When an incubator experiences a sudden loss of power there is an increased chance of condensation building up in the circulation system tubing. This mode helps to dissipate that buildup before it causes the sensors to fail Conditions for entering mode: - Incubator must be without power for 45 minutes or longer.
  • Page 40 Responding to the notification: From the main screen press the NuAire Logo / Service settings / General Option /Next / Filter Maintenance to access the Filter Maintenance screen. In these screens you can: Extend the alarm by 1 month by pushing the Last Replacement Date, date displays, extend the date by one •...
  • Page 41 11.0 Error Indicators & Troubleshooting Step 1 NOTE ALL ERROR INDICATORS. When the Incubator is running, the Alarm Status button indicates an error. Pressing the Alarm Status button, entering the correlating Alarms Menu and pressing the Silence button in the Alarms Menu will silence the audible alarm until Diagnostics is entered or another alarm becomes active.
  • Page 42 Temp Under Set Point, Faulty TRIAC, replace control board during normal running and in Faulty chamber heater contact NuAire Technical Service. Door/Perimeter heater needs to be increased with a high temperature set-point in a low ambient temperature -Sensor temperature (differential) Check temperature sensor calibration error normal running.
  • Page 43 Stops trying to write to USB Universal Serial Bus Error several tries memory stick after several tries USB port not functioning contact Nuaire technical service Increase Door heater duty cycle in 5% increments or less at a On Glass Door Condensation Excessive...
  • Page 44 12.1 Remote Alarm Contacts The NuAire DH Incubator contains a set of contact points to connect to a remote alarm system. The contacts are located on the rear panel (see page 14). The contacts are housed in a modular (RJ-11) telephone jack and rated for (30V at 1 Amp).
  • Page 45 13.0 Electrical/Environmental Requirements 13.1 Electrical (Supply voltage fluctuations not to exceed +/- 10%) NU-5800 115V, 50/60Hz, 1 Phase, 6.0 Amps NU-5800E 230V, 50/60Hz, 1 Phase, 3.0 Amps Start Up Power 345 Watts Running Power 250 Watts 13.2 Operational Performance (for indoor use only) Environment Temperature Range: 60°F-85°F (15.5°C –...
  • Page 46 13.8 Heat Rejection: 14 BTU/Minute. 14.0 Disposal and Recycle Incubators that are no longer in use and are ready for disposal contain reusable materials. ALL components with the exception of the HEPA filters may be disposed and/or recycled after they are known to be properly disinfected. ...
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This manual is also suitable for:

Nu-5800Nu-5800e

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