Chamber Temperature Calibration - NuAire NU-5510 Operation And Maintenance Manual

9.0 Calibration
Proper calibration of the DHD Autoflow involves four parameters: chamber temperature, door temperature,
perimeter temperature, and CO
completed and stabilized before any CO
is described in detail. For the best results, follow the procedure carefully, and if the desired result is not
achieved, try procedure again from the start.
9.1

Chamber Temperature Calibration

The DH Autoflow's TEMPERATURE CALIBRATION MUST BE PERFORMED WITHIN 1C OF THE
PLANNED OPERATING TEMPERATURE. Normally, 37.0C is the most common set point. To initiate
the procedure, turn on the DHD Autoflow via the power switch on the back panel. Press mode switch to
setup. The default set point is 37.0C; use the ▲▼arrows to change if desired. Press mode switch back to
run and let stabilize for 8 to 12 hours. Use an independent instrument to check and calibrate the
temperature.
Below is a description of calibration with a glass thermometer as one example for temperature calibration.
At the beginning of this procedure, set a calibrated glass thermometer in a glass beaker filled with water
resting on a shelf in the middle of the DHD Autoflow chamber. Do not place the glass beaker on the
bottom of the chamber because it will result in a slightly higher temperature reading due to the heater
located on the chamber bottom. Placing the thermometer in glass beaker on the middle shelf will give the
most accurate results for calibration. The chamber should be humidified to avoid false low readings due to
evaporation of water from the flask. An accurate digital thermometer with a type K thermal couple could
also be used.
When the unit has stabilized at the operating temperature, perform the following calibration procedure.

Allow Incubator to stabilize at its given temperature set point in run mode.

Press "SEL" to indicate LED next to temperature display.

Press and hold ▲ key for three seconds.

Determine actual temperature within chamber by reading temperature measurement instrument.

Press ▲ or ▼ key to indicate same temperature as the actual temperature being measured in the
chamber.

Press "SEL" to set current value and exit calibration.
The chamber temperature calibration is complete. Let unit stabilize for 8 to 12 hours. If the chamber
temperature (actual thermometer) still does not match the display, perform the above procedure again. In
some cases it might be necessary to calibrate several times to achieve a stable condition due to ambient
conditions of temperature and humidity within the laboratory.
OM0135
sensor. The first three, chamber, door, and perimeter temperature should be
2
sensor calibration is performed. Below, each calibration procedure
2
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