NuAire NU-4850 Maintenance Manual
NuAire NU-4850 Maintenance Manual

NuAire NU-4850 Maintenance Manual

Water-jacketed, us autoflow automatic co2 incubator

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OM0123
Rev 15
Water-Jacketed,
US Autoflow Automatic CO
NU-4750, NU-4850, NU-4950
NU-4750D, NU-4850D, NU-4950D
NU-4750E, NU-4850E, NU-4950E
Operation & Maintenance Manual
Series 10 4750
Series 5 4750D
Series 10 4750E
Series 11 4850
Series 7 4850D
Series 11 4850E
Series 11 4950
Series 7 4950D
Series 11 4950E
For 115 Vac, 60 Hz Only
Manufactured By:
2100 Fernbrook Lane
Plymouth, MN 55447
Toll-Free: 1-800-328-3352
In Minnesota: (763)-553-1270
Fax: (763)-553-0459
Page 1 of 68
Incubator
2
Models
July, 2013
Revision 15
NuAire, Inc.

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Table of Contents
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Summarization of Contents

General Description
Water-Jacket Features
Details the water-jacket's construction, insulation, capacity, and temperature uniformity.
Control Electronics System
Explains the NICE microcomputer control system, its components, and diagnostic capabilities.
CO2 Display and Control System
Describes the solid-state infrared gas analyzer for CO2 measurement and control.
Stainless Steel Construction
Details the 304L stainless steel construction, coved corners, and finishes.
RH Display and Control
Explains the humidity control system using a water vapor injection method.
O2 Display and Control (NU-4950)
Details the optional oxygen display and control system using a fuel cell type sensor.
Safety Instructions and Symbols
Covers general safety warnings, operating procedures, and explanation of symbols.
Performance Parameters
Chamber Temperature Uniformity
±0.2°C uniformity at 37.0°C due to water-jacket.
CO2 Control System
Solid-state infrared gas analyzer for accurate CO2 monitoring.
CO2 Recovery Performance
Automatic recovery to 5.0 ±0.2% CO2 within 3-1/2 minutes after door opening.
Models, Features & Specifications
Weight Specifications
Provides dry and operational weight specifications for different models.
Dimensional Specifications
Lists overall dimensions and shelf capacity details for all models.
NU-4750 Standard Features
Details standard features like stainless steel construction, water-jacket, temp/CO2 control.
NU-4850 Additional Features
Lists additional features for the NU-4850 model, including RH control.
NU-4950 Additional Features
Lists additional features for the NU-4950 model, including O2 control.
Included Items
Lists items included with the unit, such as shelves, water pan, and manual.
Optional Features List
Lists optional features like remote communication and chart recorder.
Test Performance & Procedures
Visual Inspection Procedures
Details visual checks for cleanliness, finish, and door operation.
Electrical Test Procedures
Covers electrical leakage, dielectric voltage, and grounding continuity tests.
Functional Test Procedures
Outlines tests for control systems, CO2 control, and CO2 recovery.
Shipment Handling
Damaged Shipment Claims
Provides procedures for handling visible and concealed shipping damage claims.
Installation Guide
Location Recommendations
Advises on optimal placement, avoiding heat sources and ensuring air circulation.
Leveling Procedures
Explains how to level the unit using adjustable feet for proper water circulation.
Shipping Foam Removal
Instructions to remove shipping foam from the spring pump assembly before operation.
Shelf and Water Pan Installation
Guides on installing shelves and the water pan for decontamination and operation.
Electrical Installation Requirements
Covers electrical supply requirements, grounding, and surge protection recommendations.
Water-Jacket Filling and Draining
Detailed instructions for filling with distilled water and draining the water jacket.
CO2/N2 Gas Supply Connection
Covers connecting CO2 and N2 gas supplies, including regulators and adjustments.
Incubator Operation
Chamber Sterility Maintenance
Guidance on maintaining sterility within the chamber and sterilizing components.
Humidity Control Operation
Explains humidity control for NU-4750 and NU-4850/4950 models, water type, and maintenance.
System Overview (NICE)
Overview of the NuAire Incubator Control Electronics (NICE) system and its functions.
Operator Control Interface
Explains operation via keypads, modes (Run/Setup), and parameter selection.
Automatic Alarm Notification
Describes alarm indicators (red LED) for system faults like temperature or CO2 control errors.
Front Panel Indicators and Controls
Details all indicator LEDs and control keys on the front panel.
Rear Panel Ports and Features
Describes rear panel connections for power, gas, communication, and alarms.
Run Mode Operation
Explains operator interaction in Run mode, acknowledging abnormalities.
Setup Mode Operation
Covers entering setup mode and interacting with system parameters.
Diagnostic and Troubleshooting
Guides for diagnostic tests, parameter checks, and system resets.
Calibration Procedures
Chamber Temperature Calibration
Step-by-step guide to calibrate chamber temperature using a thermometer.
Door Heater Calibration
Explains door heater duty cycle calibration for condensation control.
Adjusting Air Injections
Adjusting air injection length and frequency to control condensation.
CO2 Calibration Procedures
Covers CO2 control, sensor zero/span, and injection calibration methods.
RH Calibration (NU-4850, NU-4950)
Guide to calibrate the relative humidity sensor using a water pan.
O2 Sensor Calibration (NU-4950)
Step-by-step calibration procedure for the oxygen sensor.
Maintenance and Cleaning
Chamber Maintenance and Cleaning
Chamber maintenance, cleaning, and sterilization guidelines.
Water-Jacket Maintenance
Water-jacket maintenance, anti-bacterial agents, and material compatibility.
Filter Replacement Schedule
Guidelines for replacing CO2, O2, and Air inlet filters.
RH Reservoir Care
RH reservoir filling and maintenance instructions.
RH Sensor Cleaning
Procedures for cleaning the RH sensor and filter cap.
Incubator Shutdown Procedure
Steps for safely shutting down the incubator.
Chamber Chemical Decontamination
Methods for chemically decontaminating the chamber.
Error Codes and Troubleshooting
Troubleshooting Error Indicators
Table listing error codes, descriptions, and checks/corrections.
Memory Chip Faults
Troubleshooting for memory chip errors.
Power Supply Faults
Troubleshooting for power supply failures.
Key System Indicators
Explains Door Ajar, Self Diagnostic, and Low Water indicators.
Troubleshooting Excessive Condensation
Troubleshooting steps for excessive condensation.
Remote Alarm Contact Configuration
Details on setting up and using remote alarm contacts.
Electrical and Environmental Requirements
Electrical Specifications
Electrical specifications including voltage, power, and certifications.
Environmental Operating Conditions
Environmental operating ranges for temperature, humidity, and altitude.
Chemical Exposure Guidelines
Recommendations on cleaning agents and materials compatibility.

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