NuAire NU-5810 Operation And Maintenance Manual
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NuAire NU-5810 Operation And Maintenance Manual

Air-jacketed automatic co2 incubator
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OM0246
Rev 5 June/2020
Air-Jacketed Automatic CO
NU-5810, NU-5820, NU-5831, NU-5841
NU-5810E NU-5820E, NU-5831E, NU-5841E
Operation and Maintenance Manual
June 2020
Revision 5
For 115 Vac, 50/60 Hz Only
Manufactured By:
2100 Fernbrook Lane
Plymouth, MN 55447
Toll-Free: 1-800-328-3352
In Minnesota: (763)-553-1270
Fax: (763)-553-0459
Incubator
2
Models
Series 12
NuAire, Inc.
Page 1 of 64

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Summary of Contents for NuAire NU-5810

  • Page 1 Air-Jacketed Automatic CO Incubator Models NU-5810, NU-5820, NU-5831, NU-5841 NU-5810E NU-5820E, NU-5831E, NU-5841E Operation and Maintenance Manual June 2020 Revision 5 Series 12 For 115 Vac, 50/60 Hz Only Manufactured By: NuAire, Inc. 2100 Fernbrook Lane Plymouth, MN 55447 Toll-Free: 1-800-328-3352...

  • Page 2: Table Of Contents

    BCD-18320 ..............Tubing Diagram BCD-18317 ..............Incubator Right Hinge Door Assy BCD 18321 ..............Incubator Left Hinge Door Assy ELECTRICAL SCHEMATICS BCD-17630 ..............Electrical Schematic, NU-5810/E BCD-18007 ..............Main Control Board Electrical Schematic OM0246 Page 2 of 64 Rev 5 June/2020...

  • Page 3: Incubator

    Sterility Reliability Like all NuAire equipment, this Incubator has been designed to provide the highest quality standards of performance with matching computer technology, precise temperature control, and CO gas control system combining state-of-the-art technology with years of design, quality, and manufacturing experience.
  • Page 4 Keep these operating instructions close to the unit so that safety instructions and important information are always accessible. • Should you encounter problems that are not detailed adequately in the operating instructions, please contact your NuAire Representative or NuAire technical Services. OM0246 Page 4 of 64 Rev 5 June/2020...
  • Page 5 1.9 Explanation of symbols Symbol (in text box) Description (in text box) Safety alert symbol indicates a potentially hazardous WARNING situation which, if not avoided, could result in death of serious injury. Safety alert symbol indicates a potentially hazardous CAUTION situation which, if not avoided, may result in minor or moderate injury.
  • Page 6 Performance Parameters and Features • Both the interior and exterior of the DHD are constructed of 18 to 16-gauge steel. The interior is highly polished type 304 L 16 gage stainless steel, using crevice-free construction. All exposed edges are deburred to insure no sharp edges. The exterior is cold rolled 16 &...
  • Page 7 Models & Features 3.1 Invitrocell DHD (heated decontamination) all Models. 3.2 Dimensions (see also Specification Drawing BCD-18314) Overall Dimensions - inches (mm): Model NU-5810/E thru NU-5841/E Height: Exterior: 39.688 in (1008 mm) Width: 26.750 in (680 mm) Depth: 27.188 in (691 mm) Foot print: 23.5 in (597 mm) wide x 20.75 in (527 mm) deep...
  • Page 8 Relative Humidity Control: Available on NU-5820 and NU-5841 Performance Determined with O₂ Set Point @ 21.0% • RH Range: Low is ambient dependent to 95% maximum (default 90%) (0.0 set point idles the system) RH Accuracy: +5% / -3% • •...
  • Page 9 OM0246 Page 9 of 64 Rev 5 June/2020...
  • Page 10 Test Performance & Procedures All equipment is thoroughly inspected at the NuAire Factory at the time of shipment. Quality control is maintained by constant surveillance over the product, beginning at the receipt of purchased material and concluding with a final inspection, which certifies the Incubator performance to the specifications.
  • Page 11 However, damage can occur in any shipment and the following paragraph outlines those steps you should take on receipt of a NuAire Incubator to be sure that if damage has occurred, the proper claims and actions are taken immediately.
  • Page 12 Shelves Incubator is shipped with the shelves installed. The shelf mounted water pan is also in place that option is ordered. NuAire recommends decontaminating all surfaces within the interior chamber, glass door, and outer door with gasket. They can be wiped down with a disinfectant of 70 percent alcohol or similar non-corrosive antimicrobial agent.
  • Page 13 OM0246 Page 13 of 64 Rev 5 June/2020...
  • Page 14 7.4 Electrical The electrical supply circuit to the Incubator must conform to all national and local electrical codes. Consult the serial-data plate, located at the front of the right side of the Incubator, for voltage, cycle, phase, and ampere requirements before making connection. Plug the power cord securely into a grounded power source. VOLTAGE SHOULD NOT VARY MORE THAN 10% FROM SERIAL PLATE RATINGS.
  • Page 15 CO pressure to the Incubator. Some single stage CO pressure regulators have two gauges. USE A TWO-STAGE REGULATOR. All NuAire Incubators use CO in such quantities that precise metering of CO input pressure is important for maximum performance.
  • Page 16 7.7 Removing the cable tie used to secure the air pump for shipping: 7.8 Setting up the access port: When utilizing the access port do not block or restrict the relief holes in the center of the port plug. Blocking this venting can cause performance issues for the incubator.
  • Page 17 Using the water pan supplied with the incubator and with a 5.0% CO₂ set point. NU-5810: After a 15 second door opening RH recovers to 90% of what the RH level was prior to opening the door for 15 seconds in 20-40 minutes on average.

  • Page 18: Control System Introduction

    NOTE: DO NOT SEAL OR RESTRICT THE OPENING IN THE PORT PLUG. THIS IS A PRESSURE RELIEF FOR THE CHAMBER. 8.3 Control System Introduction The NuAire Incubator Control Electronics system is designed to serve the control requirements of the Incubator chamber. Temperature, CO levels in all models and RH & O in available models are controlled by preset values to provide the optimum conditions for culture growth within a chamber.
  • Page 19 Door ajar status displays when door is ajar • Door Closed status displays when door is closed and flashes during the post door close delay time. • NuAire Logo button Leads to the System settings menu when pressed • Date and Time •...
  • Page 20 NuTouch LCD menu system is accomplished through the following methods: • Touching a “graphic” Icon like the NuAire Logo in the Main Screen which access the System Settings menu, or lead you to an Enter Password screen when a password has been established.
  • Page 21 to set the User Password. Decon: Begins Decon setup instructions and starts the heated Decon Cycle when pressed. Also, it advances through the phases of the Decon Cycle. System Settings Screen 8.3.6 Automatic notification of abnormal situations (See Section 11 for details) The red “Alarm Status”...

  • Page 22: Nutouch Display Screen

    8.3.8 Standard remote communication capability The NU-5810 Incubator comes equipped with the following remote communication tools: • USB Output of daily, weekly & monthly performance logs and an event log that indicates when alarms, door openings and power interruptions occur.

  • Page 23: Rear Component Panel

    Indicates that the power has been interrupted. Touch text to acknowledge and Nuaire Logo will reappear. 8.4.7 USB status The USB status icon appears in the lower right side of the Main screen when a memory stick is placed in the USB port at the back of the unit while down loading performance and event history.
  • Page 24 8.6 Front Component Panel The front component panel contains the following functions. BCD-18316 OM0246 Page 24 of 64 Rev 5 June/2020...
  • Page 25 8.6.1 RH Fill Port (Available on models NU-5820 and NU-5841) The RH evaporator tank is filled through the port that has the red plug (w O-ring) screwed in it. Because of the pressure in the evaporator tank during an RH injection the O-Ring must seal against the top of the fill port. 8.6.2 N Supply filter (Available on models NU-5831 and NU-5841) The N...

  • Page 26: Run Mode Operator Interactions

    8.7 Run Mode Operator Interactions OM0246 Page 26 of 64 Rev 5 June/2020...

  • Page 27: Standby Mode Operator Interactions

    (see Section 11.0). 8.8 Standby Mode Operator Interactions The NuAire incubator automatically enters the standby mode when necessary. There is no action required by the user to enter this mode.
  • Page 28 8.8.1.3 Default Set points Temperature 37.0 C All Models Note 1: A set point of 0 (zero) will put the system into an Idle state where it does not control and all alarms are CO₂ 5.0% All Models inhibited. The sensor remains active and reports the level of gas/RH in the chamber.
  • Page 29 Diagnostic Mode To initiate the diagnostic mode, perform the following from the ‘Main’ screen: • Select the NuAire Logo to enter “System Settings” Select “Service Settings” and enter 9876, and then hit enter (ENT) • In the Service Settings menu, select the “Diagnostics” button for the desired function •...
  • Page 30 The Diagnostic screen is shown to the right of each of the control systems Temperature, CO , RH & O and the General category for your reference. Once entered, the Diagnostics screen will display the calibrated and uncalibrated values of the given function and all related components.
  • Page 31 RH Control Diagnostics functioning 1. RH Sensor This function displays the current value of the chambers RH sensor from 0 to 100%. It shows both the claibrated value shown in the display screen and un-calibrated sensor signal. 2. RH Inject Solenoid This function indicates the on/off (default off) state of the RH Inject solenoid valve and allows the activation of it.
  • Page 32 Format: Option (Default, Minimum/Maximum) All the options for the NU-5810 Incubator use this same format. The default is set at the factory and can be restored in the field with a Master or a Factory reset command. On the following pages, there is a short description of each Option with its Default/Minimum/Maximum setting, followed by a more detailed description.
  • Page 33 Temperature 1. Maximum Above Set point This value determines the maximum deviation, measured in (°C) that the chamber is permitted to rise above set point, once it is reached before an alarm condition is declared. An alarm condition will cause the chamber to enter a safe condition where no power is enabled to any of the system output controls until the situation is rectified.
  • Page 34 Relative Humidity 1. System Enable (Enabled/disabled) on models NU-5820 and NU-5841 (To Access this function: press Settings Text in Service Control Screen) This function will enable or disable the RH system. If the system is Enabled it will be displayed in the MAIN screen. If it is disabled it will not be displayed in the Main or Option/Settings screen for the RH system: System Enabled (with check mark) means the System is enabled...
  • Page 35 Screen Lock button (default Main Screen Unlocked). Pressing this button will change it to “Main screen Locked” (Lockout = 1). This puts a “Press to unlock” button on the Nuaire Logo in the Main screen. This feature stops accidental screen changes by brushing the touch screen. You must press and hold (2 seconds) until the message disappears which unlocks the main screen so that you can navigate into;...

  • Page 36: Resetting The Electronic Software

    Setting the model number sets the following function: • All Nuaire customers will press Nuaire in the “Choose Company” Screen. This will bring up the Nuaire logo in the main screen. All other customers will press the appropriate company button to bring up the identifiers specific to that company.
  • Page 37 Loads the model number in the System information screen Model numbers NU-5820 through NU-5841 and E or D models • Enables Temperature and CO plus RH and O control systems as available on the various models Chooses the algorithm suited for high watt heating of the chamber suited for heated Decon cycles. Activates the heated decon cycle choices in the “Systems Settings”...

  • Page 38: Chamber Temperature Calibration

    9.0 Calibration Proper calibration of the incubator involves following parameters. On all models of the Invitrocell incubator the chamber temperature, door temperature, perimeter temperature, and CO sensor require calibration at installation and the calibration should be checked on a regular schedule. The first three, chamber, door, and perimeter temperature should be completed and stabilized before any CO sensor calibration is performed.
  • Page 39 9.2 Door and Perimeter Duty Cycle adjustments for Temperature Calibration The Door and Perimeter heaters are run on “Base Duty Cycle” calculations that are automatically increased or decreased to run in accordance with the chamber temperature at a chosen set point. These duty cycles work in the controlled lab environment but due to the many variables involved in temperature control there is also a manual adjustment.

  • Page 40: Setting Air Injections

    9.3 Setting Air Injections If there is still some undesired condensation in the chamber when the door and perimeter heaters are set for the desired result, the air injections can be adjusted. There is a control for length of the air injection labeled, Air Inject Time, and the frequency that air is injected called, Air Inject Cycle.
  • Page 41 9.4.3 Open Door CO Sensor Calibration Routine • Attach independent CO monitoring device to the sample port on the front of the unit • Enter CO calibrations by means of Service Settings • Select the “CO Open Door Zero Span” text •...
  • Page 42 9.5.2 Calibrating with a Hygrometer When unit chamber has stabilized at the operational temperature, CO %, the RH set point has been chosen and with water in the tank the heater has been powered for at least 60 minutes ensuring it is up to temperature. Allow the RH system to achieve set point and stabilize there for at least 30 minutes.
  • Page 43 SUCH MATERIAL IS HARMFUL TO THE POLISHED STAINLESS STEEL. NOTE: NuAire does not offer any product warranty with respect to cleaning material compatibility. USE AT YOUR OWN RISK! The information provided above is from raw material suppliers and known general source documents for use to develop application cleaning SOP’s.

  • Page 44: Heated Decon Cycles

    THE HEATED DECONTAMINATION CYCLES NuAire gives the lab professional the choice of the 2 most commonly accepted, heated decontamination cycles available. They are a 95°C humidified cycle and a 145°C dry cycle. To choose, the user goes to the System Settings menu and pushes the Decon button in the lower right hand of the display.
  • Page 45 FOLLOW ALL FEDERAL, STATE AND LOCAL REGULATIONS THAT APPLY WHEN USING CLEANER. ANY RESIDUE LEFT OF THE CLEANER WILL BE EXPOSED TO THE DECON. TEMPERATURES CHECK WITH MANUFACTURER ABOUT EXPOSURE OF THE CLEANER TO THESE TEMPERATURES. DRY 145° C DECON CYCLE TIME (HRS:MIN:SEC) Note: •...
  • Page 46 • Temperature display shows chamber temperature during cycle. • Text displays that the unit is currently in the Cooling Down portion of the DECON cycle • A count up timer (incrementing time) tracks how long the unit has been cooling down CYCLE COMPLETE ...
  • Page 47 PREPARATION Following the prompts in the incubator display screen you will: Remove culture cells, samples, dishes, instruments, or other user-introduced equipment from the chamber. • • Wipe out all spills and materials from chamber walls, shelves, and plenums using a disinfectant of choice that is compatible with construction of the Incubator chamber and instrumentation.
  • Page 48 Follow the on screen instructions, pressing the Next button when each step is completed • Start the decon cycle by following the prompts on the NuTouch display screen. The decon cycle will automatically go through the following phases: HEAT UP 1 hour ...

  • Page 49: Chemical Decontamination

    10.2 Chemical Decontamination of the Incubator Chamber To chemically decontaminate NuAire Incubator inner chambers, users may use the traditional formaldehyde or Chlorine Dioxide in gas form, or Vapor Phased Hydrogen Peroxide. All three of the chemicals are compatible to all parts within NuAire Incubator chambers.
  • Page 50 Responding to the notification: From the main screen press the NuAire Logo / Service settings / General Option / Settings / Filter Maintenance to access the Filter Maintenance Adjustments screen. In this screen you can: • Extend the alarm by 1 month by pushing the Extend. Press the Exit button to retain the extension and go the General Settings screen.
  • Page 51 11.0 Error Indicators & Troubleshooting Step 1 NOTE ALL ERROR INDICATORS When the Incubator is running, the Alarm Status button indicates an error. Pressing the Alarm Status button, entering the correlating Alarms Menu and pressing the Silence button in the Alarms Menu will silence the audible alarm until Diagnostics is entered or another alarm becomes active.
  • Page 52 Decon Cycle Faulty TRIAC, replace control board Decon Temp Low - Decon Time Out Faulty chamber heater contact NuAire Technical Service. - Temp less than decon temp -4⁰ Door/Perimeter heater needs to be increased with a high for 240 minutes...
  • Page 53 RH System Displayed Error Code Code Description Checks and Corrections Perform RH sensor calibration - RH over set point beyond the alarm Check injection solenoid for leaking valve RH Level High limit Check sensor & moisture barrier in sensor cap for moisture Perform RH sensor calibration Check RH tank for water level if low check Float switch in...
  • Page 54 Stops trying to write to USB memory Universal Serial Bus Error several tries stick after several tries USB port not functioning contact Nuaire technical service Increase Door heater duty cycle in 5% increments or less On Glass Door Condensation Excessive...
  • Page 55 12.1 Remote Alarm Contacts The NuAire DHD Incubator contains a set of contact points to connect to a remote alarm system. The contacts are located on the rear panel (see page 14). The contacts are housed in a modular (RJ-11) telephone jack and rated for (30V at 1 Amp).
  • Page 56 When connecting external accessories or components to the system, CAUTION attach only components that have been tested and certified for compliance to UL/IEC 60601-1 or UL/IEC 60950 13.0 Electrical/Environmental Requirements 13.1 Electrical Models: NU-5810, NU-5820, NU-5831 and NU-5841 Domestic Units: 115V, 50/60Hz, 1 Phase, 8.5 Amps “E” Units:...
  • Page 57 13.8 Heat Rejection: 14 BTU/Minutes. 14.0 Disposal and Recycle Incubators that are no longer in use and are ready for disposal contain reusable materials. ALL components with the exception of the HEPA filters may be disposed and/or recycled after they are known to be properly disinfected. ...
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This manual is also suitable for:

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