NuAire 7 Series Maintenance Manual

Water-jacketed, us autoflow automatic co2 incubator

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OM0123

Rev 15

Water-Jacketed,

US Autoflow Automatic CO

NU-4750, NU-4850, NU-4950

NU-4750D, NU-4850D, NU-4950D

NU-4750E, NU-4850E, NU-4950E

Operation & Maintenance Manual

Series 10 4750

Series 5 4750D

Series 10 4750E

Series 11 4850

Series 7 4850D

Series 11 4850E

Series 11 4950

Series 7 4950D

Series 11 4950E

For 115 Vac, 60 Hz Only

Manufactured By:

2100 Fernbrook Lane

Plymouth, MN 55447

Toll-Free: 1-800-328-3352

In Minnesota: (763)-553-1270

Fax: (763)-553-0459

Page 1 of 68

Incubator

Models

July, 2013

Revision 15

NuAire, Inc.

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Page 1 Series 7 4850D Series 11 4850E Series 11 4950 Series 7 4950D Series 11 4950E For 115 Vac, 60 Hz Only Manufactured By: NuAire, Inc. 2100 Fernbrook Lane Plymouth, MN 55447 Toll-Free: 1-800-328-3352 In Minnesota: (763)-553-1270 Fax: (763)-553-0459 OM0123 Page 1 of 68...
Page 2: Table Of Contents
Water-Jacketed, US Autoflow Automatic CO Incubator Operation & Maintenance Manual Models NU-4750/D/E, NU-4850/D/E, NU-4950/D/E Table of Contents Section No. 1……………………………….General Description Section No. 2……………………………….Performance Parameters Section No. 3……………………………….Models, Features & Specifications Section No. 4……………………………….Test Performance & Procedures Section No. 5……………………………….Warranty Section No.
Page 3: Models Nu-4750/D/E, Nu-4850/D/E, Nu-4950/D/E
Precise O control Reliability Like all NuAire equipment, the US Autoflow has been designed to provide the highest quality standards of performance with matching computer technology, precise temperature control and CO gas control system combining state-of-the- art technology with years of design, quality and manufacturing experience.
Page 4 1.3 CO Display and Control The NuAire US Autoflow employs a solid-state single gas analyzer for carbon dioxide. This innovative analyzer utilizes a filter correlation technique for non-dispersive infrared analysis of CO . The analyzer consists of an optical bench incorporating an infrared source, sample cell, and infrared detector. The amount of power...
Page 5 • information are always accessible. Should you encounter problems that are not detailed adequately in the operating instructions, please • contact your NuAire Representative or NuAire technical Services. Explanation of symbols Symbol Description Safety alert symbol indicates a potentially hazardous...
Page 6 Chemical Hazard Ground, Earth Attention accompanies information or important symbol Potential electrical hazard, only qualified person to access. Flammable Hazard Lead Free CAUTION used without the safety alert symbol CAUTION indicates a potentially hazardous situation which, if not avoided, may result in property damage. Hot Surface Burn Potential OM0123...
Page 7 2.0 Performance Parameters Each chamber's water-jacket holds 20 gallons (75.7 liters) of water that, in conjunction with the microcomputer control system, provides an interior chamber temperature uniformity of ±0.2°C at 37.0°C. The US Autoflow's microcomputer temperature control system has two temperature sensors: one in the water jacket and one in the chamber.
Page 8 Models, Features & Specifications NuAire offers various Water-Jacketed US Autoflow Automatic models: • NU-4750/D/E*: TEMP/CO Display and Control • NU-4850/D/E*: TEMP/CO /RH Display and Control • NU-4950/D/E*: TEMP/CO /RH/O Display and Control *D: 100VAC/50-60Hz *E: 230VAC/50-60Hz Weight (lbs. /kg - per unit):...
Page 9 3.4 NU-4850 Additional Features • RH Control System (Default Set-Point 90%) • RH Range: Ambient to 95% • RH Accuracy: +3% • RH Recovery to 95% +3%: 10 minutes * 3.5 NU-4950 Additional Features • O Control System (Default Set-Point 21%) •...
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Page 11 4.0 Test Performance & Procedures All equipment is thoroughly inspected by NUAIRE at the time of shipment. Quality control is maintained by constant surveillance over each product, beginning at the receipt of purchased material and concluding with a final inspection before packing.
Page 12 Warranty NuAire, Inc. warrants that it will repair F.O.B. its factory or furnish without charge F.O.B. its factory a similar part to replace any material in its equipment within 24 months parts and labor after the date of sale if proven to the satisfaction of the company to have been defective at the time it was sold provided that all parts claimed defective shall be returned, properly identified to the company at its factory, charges prepaid.
Page 13 Close front control panel and replace side access cover and screws. Shelf & Water Pan Installation Before installation of the shelves, NuAire recommends to decontaminate all surfaces within the interior chamber, glass door and outer door with gasket. They can be wiped down with a disinfectant of 70 percent alcohol or similar non-corrosive anti-microbial agent.
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Page 15 Electrical The electrical supply circuit to the US Autoflow must conform to all national and local electrical codes. Consult the US Autoflow's serial-data plate, located on the upper corner of the right side of the Incubator, for voltage, cycle, phase and ampere requirements before making connection. Plug the power cord securely into a grounded power source.
Page 16 Air Inlet Connection An air inlet tubing kit consists of one foot clear vinyl tube and (1) 50mm polypropylene .3 micron HEPA filter. Locate the air inlet port on the back panel of the unit. Remove cover cap, connect one end of tubing to the air inlet port, and the other end of tubing to the air filter.
Page 17 0 to 30 PSI or 0 to 2 BAR (100 PSI or 6 BARS maximum). This gauge will indicate the actual CO pressure into the US Autoflow system. Some single stage CO pressure regulators have two gauges. USE A TWO STAGE REGULATOR. All NuAire Autoflow's use CO in such small quantities that precise metering of CO input pressure is important for maximum performance of the US Autoflow.
Page 18 Humidification of the US Autoflow Model NU-4750 Series is achieved through the process of water evaporation (vapor pressure) from a stainless steel water pan (NuAire Model NU-1555) placed near the bottom of the US Autoflow shelf rack. Materials of different thermal resistance (i.e. glass, plastic) do not offer sufficient thermal recovery and are not recommended for use.
Page 19: System Introduction
9.4 before determining the RH set-point and/or calibrating the system. 8.3 System Introduction The NuAire Incubator Control Electronics (NICE) system is designed to service the control requirements of the US Autoflow Incubator chamber. Temperature and CO level are controlled by preset values to provide optimum conditions for culture growth within a chamber.
Page 20 Simplified Operator Controls. The control panel is operated using five keypads, run/setup key, Up/Down arrow keys, Select key and Hidden key (NuAire logo). The run/setup key controls the Incubator's two modes, Run or Setup. To change modes, press and hold the run/setup key for three seconds.
Page 21 8.3.5 Provision for add-on expansion capability. Options include: • Remote communication capability (RS-232) • Chart recorder output (0-10VDC) of individual monitored parameters • Automatic tank switch (es) 8.3.6 Diagnostic and calibration assists. By pressing hidden key, diagnostic mode is entered. In this mode: •...
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Page 25 8.4.10 RH Inject Status LED (NU-4850 and NU-4950) The control RH green LED indicates when the RH control valve is open and water vapor is flowing into the chamber. 8.4.11 RH Reservoir Status LED (NU-4850 & NU-4950) The reservoir yellow LED indicates one of two conditions. 1.
Page 26: Us Autoflow Rear Panel
8.5 US Autoflow Rear Panel The US Autoflow rear control panel contains the following functions described in detail. (See Drawing BCD-07347) 8.5.1 Power Cord The power cord is an 8-foot (2 m) in length, type "SVT" molded plug, allowing for long life and easy clean ability.
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Page 28: Run Mode Operator Interactions
8.6 Run Mode Operator Interactions In general, there is no need for operator interaction in "RUN" mode. However, operator interaction is required to perform calibration functions and abnormal condition status. If an abnormal condition has, or does, exist for a particular parameter, a blinking green LED next to the parameter will be lit. This could be a catastrophic alarm condition, which could harm the tissue culture cells.
Page 29 8.7.1.1 Automatic Adjustment of Post Inject Delay Times Post inject delays are automatically adjusted / RH according to the O2 set point to facilitate gas ADJUSTED ADJUSTED and RH recovery after a door opening. The POIN DELAY DELAY value displayed in the option menu will not TIME TIME change but is the basis for a formula in the...
Page 30: Diagnostic Interactions
• Rst - reset, master To initiate the diagnosis mode, perform the following: a) Press and hold Hidden key (flag on NuAire logo) for four seconds (in either Run or Setup mode), the temperature display will indicate the first menu “tst”...
Page 31 Once you have made your menu selection, you will remain within that menu selection until you exit the diagnostic mode. If another menu selection is desired, you must re-enter the diagnostic mode via the hidden key. To exit the function parameters, press the hidden key (flag on NuAire logo). The following is a description of each function parameter.
Page 32 Chamber Temperature Sensor This function shows the current value of the chamber temperature sensor on the temperature display. This function also allows the jacket heater to be turned on at different percentages (0, 25, 50, 75, 100) alternating with the temperature (function 2 – Safety Relay should be “NO” to force heater output).
Page 33 10. CO Tank 2 Valve This function shows the current state of the CO tank 2 valve. The CO percent display will show “on” or “off” corresponding to the valve condition. 11. N-Gas Tank 2 Valve This function shows the current state of the N-Gas tank 2 valve. The CO percent display will show “on”...
Page 34 8.8.4 Option Configuration Parameters Option Configuration Parameters Current System Enable (on/off) Tank 2 Enable (on/off) Auto Switch Back (on/off) -Closed Door CO Zero/Span Calibration (on/off) -RH System Enable (on/off) -HC System Enable (on/off) System Enable (on/off) -N-Gas Tank 2 Enable (on/off) -N-Gas Tank Switch Back Enable (on/off) -Password (on/off) Display Delay (on/off)
Page 35 To exit the function parameters, press the hidden key (flag on NuAire logo). Following are descriptions of each control parameter. 1. CO System Enable This function will enable or disable the CO system. The value display will show “on” or “off” corresponding to the current condition.
Page 36 7. O System Enable (NU-4950 only) This function will enable or disable the optional O system. The value display will show “on” or “off” corresponding to the current condition. In Run mode, the O percent will indicate either the O percent when the system is on or blank when the system is off.
Page 37 12. Audible Alarm Enable This function will enable or disable the audible alarm ringback function. The value display will show "ON" or "OFF" corresponding to the current condition. If the function is "ON", the audible alarm will provide a ringback of the alarm condition. If the user pushes any key to silence the audible alarm, after 15 minutes of silence the audible alarm will return.
Page 38 17. RH Inject Delay Time (NU-4850 & 4950) This value specifies the time, in seconds, for an injection of RH to be measurable at the sensor. When RH is injected into the chamber, the system delays until this period has elapsed before making a new control decision.
Page 39 23. Temperature Time Out This value determines the time, in minutes, for the temperature to achieve setpoint. If the temperature doesn't get to within 0.2° of setpoint within this time period, an alarm condition is declared. 24. CO Time Out This value determines the time, in minutes, for the CO percentage to achieve setpoint.
Page 40 28. Temperature Maximum Above Setpoint This value determines the maximum deviation, measured in temperature (°C), that the chamber is permitted above once the Incubator reaches the specified setpoint before an alarm condition is declared. An alarm condition will cause the chamber to enter a safe condition where no power is enabled to any of the system output controls until the situation is rectified.
Page 41 8.8.5.1 Reset, Master The master reset diagnosis function is the last effort to correct operational faults, which otherwise cannot be solved. By reloading the default configuration, the entire memory will be reset and ALL CALIBRATION OFFSETS AND CONFIGURATION OPTIONS WILL BE LOST. ALL CURRENT RUN PARAMETERS WILL BE RESET TO DEFAULT VALUES.
Page 42: Chamber Temperature Calibration
9.0 Calibration Proper calibration of the US Autoflow involves four parameters: chamber temperature, door temperature, CO sensor, humidity, and O sensors (NU-4850, 4950). The first two, chamber and door temperature should be completed and stabilized before any CO /humidity/O sensor calibration is performed. Below, each calibration procedure is described in detail.
Page 43 9.2 Door Temperature Calibration This calibration is provided to control condensation on the inner glass door and help maintain chamber temperature uniformity over the range of ambient temperatures and chamber temperature set-points that the Incubator is designed to handle. The door heater operates on a duty cycle that is a percentage of the time that the heater is turned on.
Page 44 The following steps should be taken for setting these duty cycle percentages: • Allow Incubator to stabilize at its given temperature and humidity level. • In run mode, press "SEL" to indicate LED next to temperature display. • Press and hold ▲ and ▼ keys simultaneously for three seconds. Temperature displays "dor"...
Page 45 When unit has stabilized at the operational temperature and CO percentage, take an independent measurement and, if necessary, perform the following: • In run mode press "SEL" to indicate LED next to CO percent display. • Press and hold ▲ key, CO display alternates between “ADJ”...
Page 46 Note: The CaL inject rate is automatically calculated from the CO injection made during the span calibration making it unnecessary to run the separate “CAL InJection” calibration. The injection is dependent on the proper gas pressure and factory set flow rate. Any changes in either will result in a change to the value reached during this injection.
Page 47 9.4.3 Injection Calibration The CO injection calibration can be performed separately from zero/span calibration to optimize the gas injection time required to recover the CO level to set point after a door opening. The recovery time should be as minimal as possible with virtually no overshoot. CO injection calibration is required to be performed only after a CO sensor closed door calibration.
Page 48: Relative Humidity Calibration (Nu-4850, 4950)
9.5 Relative Humidity Calibration (NU-4850, NU-4950) 9.5.1 Relative humidity calibration can be performed anytime if the relative humidity option has been installed. The relative humidity sensor can be calibrated from a known source of humidity within the Incubator chamber. Typically, the water pan is used because it has a known minimum humidity level of 96 percent after 12 hours.
Page 49 the sensor to control the humidity. Any set-point chosen above 90% can lead to the actual relative humidity reaching saturation before the system set-point is achieved. For example a 92% set-point is chosen. The actual chamber humidity can reach saturation (96%) before the sensor tells the Incubator that the set-point has been reached.
Page 50 2. The moisture from Cell culture media in unsealed containers will affect the humidity in the chamber. The greater the number of unsealed containers of media in the chamber the greater the effect becomes and can cause humidity alarms if humidity levels are pushed above the RH set-point.
Page 51 10.0 Maintaining Your US Autoflow US Autoflow Chamber The chamber maintenance is up to the discretion of the owner and the extent of cleanliness and sterility desired. The shelves and bracket supports are all removable and autoclavable. The interior should be wiped down with an appropriate disinfectant such as 70% ISOPROPYL ALCOHOL or equivalent.
Page 52 10.2 Chemical Decontamination of the Incubator Chamber To chemically decontaminate NuAire Incubators, users may use the traditional formaldehyde, Vapor based Hydrogen Peroxide, or Chlorine Dioxide. All three of the chemicals are compatible to all parts within NuAire Incubators. NOTE: As stated previously, the chamber and components can also be wiped down with a 70% solution of Isopropyl Alcohol for cleaning and decontamination.
Page 53 1. Check gas supply then run calibration again - Cal inject calibration failed. Not enough 2. Call NuAire Technical Services if error persists increase in the CO reading after gas was injected 1. Zero span calibrate the CO sensor - Auto zero failure.
Page 54: Sensor Calibration (Nu-4950)
RH Percent 1. Check for other sources of humidity in the chamber, i.e. excessive condensation and remove. - RH over setpoint 2. Check inject valve for leaking. 3. Check the shuttle valve and replace if faulty. 4. Perform RH sensor calibration. 1.
Page 55 Memory Chip Fault 1. Turn Incubator off and back on. 2. If CrC message persists, push “NUAIRE” button to reset. - Corrupted memory data All systems will require recalibration. read at start-up 3. Continuation of 2, refer to Section 9.
Page 56 For further assistance, call NuAire Customer Service at 1-800-328-3352 or (763) 553-1270 USA. 12.0 Remote Alarm Contacts The NuAire US Autoflow provides a set of relay contacts to monitor alarm via RJ-11 phone type connection on the back of the unit. The remote alarm contacts provide N.C, N.O outputs and ground.
Page 57 13.0 Electrical/Environmental Requirements 13.1 Electrical START UP POWER RUNNING POWER Model Hertz Phase Amps Watts Watts UL/UL-C Listed NU-4750 NU-4850 NU-4950 115V 60Hz NU-4750D NU-4850D NU-4950D 100V 50/60Hz CE Certified NU-4750E NU-4850E NU-4950E 230V 50Hz 13.2 Operational Performance - Indoor Use Only Environment Temperature Range: 60°F-85°F (15°C - 30°C) Environment Humidity: 20% - 60% Relative Humidity...
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NuAire 7 Series Maintenance Manual

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