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NuAire 1 Series Operation And Maintenance Manual
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NuAire 1 Series Operation And Maintenance Manual

Air-jacketed dh invitrocell co2 incubator
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Air-Jacketed DH Invitrocell CO
Incubator
2
Models
NU-5700 (Series 1)
NU-5700E (Series 1)
Operation and Maintenance Manual
Oct 2016
Revision 1
For 115 Vac, 50/60 Hz Only
Manufactured By:
NuAire, Inc.
2100 Fernbrook Lane
Plymouth, MN 55447
Toll-Free: 1-800-328-3352
In Minnesota: (763)-553-1270
Fax: (763)-553-0459
OM0250
Page 1 of 54
Rev 1 October/2016

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Summary of Contents for NuAire 1 Series

  • Page 1 NU-5700 (Series 1) NU-5700E (Series 1) Operation and Maintenance Manual Oct 2016 Revision 1 For 115 Vac, 50/60 Hz Only Manufactured By: NuAire, Inc. 2100 Fernbrook Lane Plymouth, MN 55447 Toll-Free: 1-800-328-3352 In Minnesota: (763)-553-1270 Fax: (763)-553-0459 OM0250 Page 1 of 54...

  • Page 2: Table Of Contents

    Air-Jacketed DHD Invitrocell CO₂ Incubator Operation and Maintenance Manual NU-5700 & NU-5700E Table of Contents Section No. 1 ..........General Description Section No. 2 ..........Performance Parameters and Features Section No. 3 ..........Models & Features Section No. 4 ..........Test Performance & Procedures Section No.

  • Page 3: Sterility

    Sterility Reliability Like all NuAire equipment, this Incubator has been designed to provide the highest quality standards of performance with matching computer technology, precise temperature control, and CO gas control system combining state-of-the-art technology with years of design, quality, and manufacturing experience.
  • Page 4 Keep these operating instructions close to the unit so that safety instructions and important information are always accessible.  Should you encounter problems that are not detailed adequately in the operating instructions, please contact your NuAire Representative of NuAire technical Services. OM0250 Page 4 of 54 Rev 1 October/2016...
  • Page 5 1.9 Explanation of symbols Symbol Description Safety alert symbol indicates a potentially hazardous WARNING situation which, if not avoided, could result in death of serious injury. Safety alert symbol indicates a potentially hazardous situation which, if not avoided, may result in minor or CAUTION moderate injury.
  • Page 6 2.0 Performance Parameters and Features  Both the interior and exterior of the DH are constructed of 18 to 16-gauge steel. The interior is highly polished type 304 L 16 gage stainless steel, using crevice-free construction. All exposed edges are deburred to insure no sharp edges. The exterior is cold rolled 16 &...
  • Page 7 3.0 Models & Features 3.1 Dimensions (see also Specification Drawing BCD-18412) Overall Dimensions - inches (mm): Model NU-5700/E Height: Exterior: 36.188 inches (894 mm) Width: 25.500 inches (648 mm) Depth: 27.500 inches (699 mm) Foot print: 22.625 inches (575 mm) wide x 20.825 inches 530 mm) deep Height: Interior: 24.000 inches (610 mm) Width:...
  • Page 8 3.3 Standard Items Packed With Unit  Four (4) stainless steel shelves  Eight (8) stainless steel shelf brackets  One (1) water pan  Gas supply tube(s)  Access port plug - vented  One (1), 2 meter (6.5 ft.) electrical cord 10 Amp 18awg with 70⁰C rated jacket * ...
  • Page 9 OM0250 Page 9 of 54 Rev 1 October/2016...
  • Page 10 4.0 Test Performance & Procedures All equipment is thoroughly inspected at the NuAire Factory at the time of shipment. Quality control is maintained by constant surveillance over the product, beginning at the receipt of purchased material and concluding with a final inspection, which certifies the Incubator performance to the specifications.
  • Page 11 5.0 Warranty NuAire, Inc. warrants that it will repair F.O.B. its factory or furnish without charge F.O.B. its factory a similar part to replace any material in its equipment within 24 months after the date of sale if proven to the satisfaction of the company to have been defective at the time it was sold provided that all parts claimed defective shall be returned, properly identified to the company at its factory, charges prepaid.
  • Page 12 7.3 Shelf & Water Pan Installation Before installation of the shelves, and water pan, NuAire recommends to decontaminate all surfaces within the interior chamber, glass door, and outer door with gasket. They can be wiped down with a disinfectant of 70 percent alcohol or similar non-corrosive antimicrobial agent.
  • Page 13 OM0250 Page 13 of 54 Rev 1 October/2016...
  • Page 14 7.4 Electrical The electrical supply circuit to the Incubator must conform to all national and local electrical codes. Consult the serial-data plate, located at the front of the right side of the Incubator, for voltage, cycle, phase, and ampere requirements before making connection. Plug the power cord securely into a grounded power source. VOLTAGE SHOULD NOT VARY MORE THAN 5% FROM SERIAL PLATE RATINGS.
  • Page 15 0 to 2 BAR (100 PSI or 6 BARS maximum). This gauge will indicate the actual CO pressure to the Incubator. Some single stage CO pressure regulators have two gauges. USE A TWO-STAGE REGULATOR. All NuAire Incubators use in such small quantities that precise metering of CO input pressure is important for maximum performance.
  • Page 16 7.7 Water Pan Place water pan in the center on the bottom of the chamber and fill with Single distilled water no purer than 1 Mega Ohm. It is recommended to fill the pan to about 1/2 inch below the top rim. See Sections 3.1 and 8.2 for more specifications and operational details.
  • Page 17 8.0 DH Operation ATTENTION ACCOMPANY'S POTENTIAL ELECTRICAL HOT SURFACE INFORMATION OR IMPORTANT HAZARD ONLY QUALIFIED BURN POTENTIAL SYMBOL PERSON TO ACCESS Safety alert symbol indicates potentially hazardous CAUTION Situation which, if not avoided, may result in minor Or Moderate injury. CAUTION: A qualified technician who is familiar with the proper maintenance procedures required for this equipment, as well as repair must perform all maintenance actions on this equipment.

  • Page 18: Control System Introduction

    8.3 Control System Introduction The NuAire Incubator Control Electronics system is designed to serve the control requirements of the Incubator chamber. Temperature and CO levels are controlled by preset values to provide the optimum conditions for culture growth within a chamber.
  • Page 19 NU-Touch LCD menu system is accomplished through the following methods:  Touching a “graphic” Icon like the NuAire Logo in the Main Screen which access the System Settings menu, or lead you to an Enter Password screen when a password has been established.
  • Page 20 8.3.5 Functioning accessed from the System Settings screen: The “System Settings” menu screen can be accessed from the “Main Screen” by touching the NUAIRE logo: Environmental Set-Points: Allows adjustment of Temperature, CO2, Door, and Perimeter heater power level settings. Note: You can also access the Environmental set points by touching any control system numerical value in the MAIN SCREEN.
  • Page 21 8.3.9 Diagnostic and calibration assists. To access the Service Settings Menu, perform the following steps: NuAire Logo → Service Settings → 9876 Enter. You are now in the Service Settings Menu (screen). This screen provides you the capability to review and analyze numerous Incubator functions including the following: ...
  • Page 22 The “Alarm Status” button indicates is triggered when a timer for a maintenance reminder runs to zero. This text appears in red just below the NuAire Logo (paragraph 8.4.2 and 8.4.3). The “Alarm Status” button and audible alarm indicates the abnormality. A catastrophic temperature control condition will de-energize the safety relay and cause the chamber to cool below the set point.
  • Page 23 8.5 Rear Panel Detail The rear control panel contains the following functions (BDC-15669) 8.5.1 Power Cord The power cord is 6.5-foot (2 m) in length, type “SVT” molded plug, allowing for long life and easy cleaning. 8.5.2 Circuit Breaker All control electronics are protected with a circuit breaker that may trip at 110% of load rating, but will trip at 145% of load rating in less than 2 seconds.
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  • Page 25 8.6 Front Component Panel The front component panel contains the following functions. See BCD-18316 8.6.1 CO2 Supply Filter The CO2 supply filter is installed at the factory and should be changed following the instructions found in Maintenance Section 10. 8.6.2 Air Inlet Filter - All models The Air Inlet Filter is installed at the factory and should be changed following the instructions found in Maintenance section 10.
  • Page 26 OM0250 Page 26 of 54 Rev 1 October/2016...

  • Page 27: Run Mode Operator Interactions

    8.8 Standby Mode Operator Interactions The NuAire incubator automatically enters the standby mode when necessary. There is no action required by the user to enter this mode. All functions that put the incubator in standby mode automatically start a 10 minute timer. If the function is not interfaced through the screen on the NU-Touch display the incubator then returns to the MAIN SCREEN and switches to RUN mode.
  • Page 28 The diagnostic mode allows the operator to configure and/or check the Incubator for input/output signals manually and individually. To initiate the diagnostic mode, perform the following from the ‘Main’ screen:  Select the NuAire Logo to enter “System Settings”  Select “Service Settings” and enter 9876, and then hit enter (ENT) ...
  • Page 29 Output/Input Diagnostic Parameter Functions Temperature 1. Safety Relay (on/off) 2. Chamber Temp. Sensor (shows current temp.) 3. Safety Temp. Sensor (shows current temp.) 4. Chamber heater Triac control (0 - 50% - 100%) 5. Door Heater Triac control (0 - 50% - 100 %) 6.
  • Page 30 5. Door Heater This function shows on LCD the current state of the door heater. This function also allows the door heater to be turned on at different percentages (0, 50, and 100 percent). The LCD display will show phase firing percentage chosen default is 0%.
  • Page 31 8.10 Option Configuration Parameters Format: Option (Default, Minimum/Maximum) All the options for the NU-5700 Incubator use this same format. The default is set at the factory and can be restored in the field with a Master or a Factory reset command. On the following pages, there is a short description of each Option with its Default/Minimum/Maximum setting, followed by a more detailed description.
  • Page 32 Screen Lock button (default Main Screen Unlocked). Pressing this button will change it to “Main screen Locked” (Lockout = 1). This puts a “Press to unlock” button on the Nuaire Logo in the Main screen. This feature stops accidental screen changes by brushing the touch screen. You must press and hold (2 seconds) until the message disappears which unlocks the main screen so that you can navigate into;...
  • Page 33 To perform a Factory Reset, you will need to do the following: NuAire Logo → Service Settings → 9876 Enter → Reset → Factory Reset → Confirm The Incubator will perform the Factory Reset and return to the Main screen.
  • Page 34 8.11.3 Inputting the Factory settings: The “Factory Set-up” screen will appear after the master reset. If the Model Number or the Serial Number need to be re-input for any reason the following steps should be taken: Setting the model number sets the following function: ...

  • Page 35: Chamber Temperature Calibration

    9.0 Calibration Proper calibration of the incubator involves four parameters: chamber temperature, door temperature, perimeter temperature, and CO sensor. The first three, chamber, door, and perimeter temperature should be completed and stabilized before any CO sensor calibration is performed. Below, each calibration procedure is described in detail. For the best results, follow the procedure carefully, and if the desired result is not achieved, try procedure again from the start.
  • Page 36 9.2 Door and Perimeter Duty Cycle adjustments for Temperature Calibration The Door and Perimeter heaters are run on “Base Duty Cycle” calculations that are automatically increased or decreased to run in accordance with the chamber temperature at a chosen set point. These duty cycles work in the controlled lab environment but due to the many variables involved in temperature control there is also a manual adjustment.
  • Page 37 9.2.1 Door and Perimeter heater duty cycle automatic control The door and perimeter duty cycles are automatically reduced when the room temperature in the lab increases enough to allow the contribution from these heaters to overheat the chamber. For example if the door and perimeter duty cycles are set up when the room temperature is 22C and the room temperature is allowed to increase to 27C.
  • Page 38 9.4 CO Calibration The DH infrared CO sensor may be calibrated using one of four techniques: Open Door CO , Closed Door CO2, Off-Set, and CO2 injection calibrations. CO2 calibrations can be performed in approximately ten minutes, by accessing the CO2 calibrations menu in “Service Settings”...
  • Page 39 9.4.3 Open Door CO Sensor Calibration Routine  Attach independent CO2 monitoring device to the sample port on the front of the unit  Enter CO2 calibrations by means of Service Settings  Select the “CO2 Open Door Zero Span” text ...

  • Page 40: Chemical Decontamination

    10.1 Chemical Decontamination of the Incubator Chamber To chemically decontaminate NuAire Incubator inner chambers, users may use the traditional formaldehyde or Chlorine Dioxide in gas form, or Vapor Phased Hydrogen Peroxide. All three of the chemicals are compatible to all parts within NuAire Incubator chambers.
  • Page 41 Maintenance Required button produces a screen indicating that the timer for filter life has expired. Responding to the notification: From the main screen press the NuAire Logo / Service settings / General Option / Settings / Filter Maintenance to access the Filter Maintenance Adjustments screen.
  • Page 42 11.0 Error Indicators & Troubleshooting Step 1 NOTE ALL ERROR INDICATORS. When the Incubator is running, the Alarm Status button indicates an error. Pressing the Alarm Status button, entering the correlating Alarms Menu and pressing the Silence button in the Alarms Menu will silence the audible alarm until Diagnostics is entered or another alarm becomes active.
  • Page 43 Temp Under Set Point, Faulty TRIAC, replace control board during normal running and in Faulty chamber heater contact NuAire Technical Service. Door/Perimeter heater needs to be increased with a high temperature set-point in a low ambient temperature -Sensor temperature (differential) Check temperature sensor calibration error normal running.
  • Page 44 Remove top plenum and ensure that blower wheel is not rubbing on anything. Check jacket fan mounted in bottom on Incubator For further assistance, call NuAire Customer Service at 1-800-328-3352 or (763) 553-1270 USA. OM0250 Page 44 of 54 Rev 1 October/2016...
  • Page 45 12.1 Remote Alarm Contacts The NuAire DH Incubator contains a set of contact points to connect to a remote alarm system. The contacts are located on the rear panel (see page 14). The contacts are housed in a modular (RJ-11) telephone jack and rated for (30V at 1 Amp).
  • Page 46 13.0 Electrical/Environmental Requirements 13.1 Electrical (Supply voltage fluctuations not to exceed +/- 10%) NU-5700 115V, 50/60Hz, 1 Phase, 4.1 Amps NU-5700E 230V, 50/60Hz, 1 Phase, 2.1 Amps Start Up Power 435 Watts Running Power 250 Watts 13.2 Operational Performance (for indoor use only) Environment Temperature Range: 60F-85F (15.5C –...
  • Page 47 14.0 Disposal and Recycle Incubators that are no longer in use and are ready for disposal contain reusable materials. ALL components with the exception of the HEPA filters may be disposed and/or recycled after they are known to be properly disinfected. ...
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This manual is also suitable for:

Nu-5700Nu-5700e