Cleaning And Decontamination; Decontamination Procedure; Wipe/Spray Disinfection - Thermo Scientific MSC-Advantage Operating Instructions Manual

Operating Instructions Biological Safety Cabinet | MSC-Advantage
9

Cleaning and decontamination

9.1

Decontamination procedure

Several procedures can be applied for decontaminating the biological safety cabinet.
Which procedure is selected, depends on:
• the potential risk imminent in the agents,
• the degree of purity required by an experiment or by a work process.
Possible decontamination procedures:
Wipe/spray disinfection: is the standard disinfection procedure for cabinets
used for microbiological experiments.
UV disinfection: is particularly suited as an intensifying additional disinfection
after a wipe/spray disinfection.
Sterilization with steam: can be used for treating the removable stainless steel
components. Examples of autoclavable components are the bezels of the UV
lamps, the workplate or workplate segments, the armrests and the protective
grid (see Section 9.6 / 9.7).
Disinfection with formaldehyde: can be performed if a sterile sample chamber
is required for the work process. This sterilization procedure is mandatory:
• when filters are replaced,
• when the device is shut down,
• when the device is discarded.
9.2

Wipe/spray disinfection

The wipe/spray disinfection is performed in three stages:
• predisinfection,
• cleaning,
• final disinfection.
Recommended disinfectants:
38
NOTE – Compatibility!
Chloride-containing disinfectants may damage
some surfaces, Therefore, use only chloride-
free disinfectants or a disinfectant with a low
enough chloride content to have been proved
harmless for stainless steel finishes!
Disinfectants with an alcohol content of more
than 70 % may cause embrittlement of plastic
components after extended exposure. Use only
disinfectants with a low alcohol content. When
using a disinfectant with an alcohol content
of more than 70 %, the release limit of 200 g
within 2 hours must not be exceeded.
Also suited are disinfectants based on quater-
nary ammonium compounds.