Abnormal Baseline Troubleshooting - Waters 2410 Operator's Manual

Table 7-1 Abnormal Baseline Troubleshooting
Baseline drift, rapid
7
Baseline drift, slow
Baseline drift, descending
7-4 Troubleshooting
Symptom
Column not equilibrated
Detector not allowed to
warm up
Solvent contaminated or
not HPLC grade
Solvent not properly
degassed (rapid or slow
drift)
Tubing contaminated
Flow fluctuations (rapid or
slow drift)
Solvent contaminated
Ambient temperature
fluctuations
Dirty flow cell
Leaky flow cell
Possible Cause
Equilibrate column.
Allow detector to warm up
until baseline is stable.
Warm-up time varies based
on sensitivity.
Use fresh solvent.
Degas the solvent. Cap the
solvent reservoir.
Clean the tubing using the
procedure in Section 6.1,
Cleaning the Fluidic Path.
Fix pump problems, replace
pump seals, check valves.
Use fresh, degassed solvent.
Stabilize operating
environment temperature
enough to allow full
equilibration. Keep the
system away from air
conditioning vents, chance
breezes, and direct sunlight.
Clean flow cell (Section 6.1,
Cleaning the Fluidic Path).
Call Waters Technical
Service.
Corrective Action