Selecting Parameter Values; Sensitivity Guidelines - Waters 2410 Operator's Manual

2. Press Enter.
When you perform the Auto Zero command, the letters AX appear on the display.
When you perform the Mark command, the letters CH appear on the display.
When you perform the Purge command, the letters PgE appear on the display.
To stop purging, press 2nd Func, Purge, then press Enter. The display returns to the function it
displayed before the purge began.

5.2 Selecting Parameter Values

You can adjust the noise level, peak height, peak direction, and the temperatures of the internal
oven and column heaters to optimize the performance of the 2410 refractometer. This section
provides guidelines and considerations for selecting parameter values that are best suited to your
application. The parameters are:
• Sensitivity (Sens key)
• Scale factor
• Time constant (Filter key)
• Temperature (Ext1 °C, Ext2 °C, Int °C, Set °C keys)
• Polarity (+/– key)

5.2.1 Sensitivity Guidelines

Sensitivity affects the magnitude of the output signal to an integrator or a recorder. Increasing the
sensitivity (Sens) setting increases the resulting peak areas, but it also increases baseline noise and
the response to environmental fluctuations. In addition, an increase in sensitivity reduces the
dynamic range over which the refractometer output is useful. Refer to Figure 5-2 for examples of
the effects of varying the Sens setting on a chromatogram.

Selecting Parameter Values

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