Incorrect Qualitative/Quantitative Results - Waters 2410 Operator's Manual

Table 7-3 Resolution Troubleshooting (Continued)
Symptom
Flat-topped peaks

7.2.4 Incorrect Qualitative/Quantitative Results

If a peak is incorrectly identified by a data system or integrator, make sure that the retention time is
correct.
If retention times are correct and peak resolution is good, the cause of qualitative and quantitative
errors is not likely to be chromatographic; it is more likely due to inadequate sample preparation or
faulty processing of the data (integration).
To troubleshoot problems with qualitation and/or quantitation, refer to Table 7-4.
Possible Cause
Detector not zeroed
Incorrect recorder input
voltage
Sensitivity too high
Scale factor too high
(recorder only)
Sample concentration or
injection volume exceeds
voltage output of detector
Chromatography Troubleshooting 7-11
Corrective Action
Auto zero detector baseline.
Adjust recorder input
voltage, or adjust detector
output cable to correct
position.
Select a lower sensitivity.
Select a lower scale factor.
Decrease sample
concentration or injection
volume.
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