Optimizing The Ms Detector Tune Automatically With Your Analyte - Thermo Scientific LTQ XL Getting Started

Optimizing the MS
Detector Tune
Automatically with
Your Analyte
Thermo Electron Corporation
Optimize the MS detector tune automatically to maximize the ion
transmission of reserpine (or your analyte of interest) for a high-flow
experiment. Thermo Electron recommends that you begin optimizing after
you have successfully passed an automatic tuning procedure and an
automatic calibration procedure with the calibration solution infused at
5 μL/min.
The following procedure describes how to optimize the MS detector Tune
Method with the reserpine m/z 609.2 peak at an LC flow rate of
400 μL/min. You can also carry out this procedure with your analyte of
interest and at your particular LC flow rate. (Refer to
about setting flow rates and temperatures.)
Optimize the MS detector Tune Method
1. On the Control/Scan Mode toolbar, click the Tune button to display
the Tune dialog box.
2. If necessary, click the Automatic tab to display the Automatic tuning
page. See Figure 33.
3. In the What to Optimize On group box, select the Mass option button
to make active the Mass spin box.
4. In the Mass spin box, enter 609.2 (or the appropriate mass of your
analyte of interest) to specify that the LTQ XL MS detector is to use the
peak at m/z 609.2 (or the appropriate mass of your analyte of interest) to
optimize your tune.
5. Ensure that the Divert/Inject valve is in the Detector position, as
follows:
a. Click the Divert/Inject button to open the Divert/Inject Valve
dialog box. See
b. Click the Detector option button.
c. Click Close.
6. Start the automatic tuning procedure from the Tune dialog box, as
follows:
a. Click Start. A message box displays the following message:
Please ensure that the 500 microliter syringe is full.
4
Tuning with Your Analyte in LC/ESI/MS Mode

Optimizing the MS Detector Tune Automatically with Your Analyte

Figure 34.
Table 2 for guidelines
LTQ XL Getting Started 77