Nikon eclipse lv150 Instruction Manual page 19

When configured with the LV-UEPI2
1. Turn on the power.
2. Set the microscope for
bright-field microscopy.
If accessories for DIC or
polarization microscopy
(*1 to *3) are in place,
pull them out of the
optical path.
3. Place the sample on the
stage and focus on it.
(p.27)
4. Adjust the angle of the
binocular part. (p.29)
5. Adjust the diopter. (p.30)
6. Adjust the interpupillary
distance. (p.30)
7. Change the magnification
and observe the sample.
Hint:
It may be difficult to
focus on a sample with
small contrast, such on a
polished surface. In a
case like this, stop down
the field diaphragm so
that its image can be
seen in the viewfield,
and try to focus on the
rim of the diaphragm
image. When the rim is
in focus, the sample is in
focus just as well.
Push in.
1
Binocular eyepiece: 100% (P.29)
Turn the
microscopy
selection knob.
2
BF (bright-field) (P.33)
*2
Select the
3
10x objective.
On the LV150A,
use the nosepiece
rotation buttons. (p.35)
Lower the stage
4 as far as it will go.
Coarse focus knob
(p.27)
Adjust the
5 brightness.
Brightness control
knob (p.26)
Select the desired
1
magnification.
On the LV150A,
use the nosepiece
rotation switch.
(p.35)
Finely adjust
2
the focus.
Coarse/fine focus
knob (p.27)
Adjust the
brightness.
3
Brightness control
knob (p.26)
To fully open the field and
P
A. ST O
P
F. ST O
BF
*3
*1
Adjust to circumscribe
Image of field diaphragm
Adjust to 70 to 80% of
P
A. ST O
P
F. ST O
BF
II. Microscopy
Raise the levers.
6
aperture diaphragms.
(p.31 and p.32)
Push in the
NCB11 filter.
7
To compensate
color temperature.
(p.26)
Adjust the
brightness.
8
ND filter
(p.26)
Power switch
the viewfield.
4
Field diaphragm (p.31)
Viewfield
the objective's N.A.
5
Aperture diaphragm
(p.32)
Objective's
pupil
Image of
aperture
diaphragm
Adjust
the brightness.
6
ND filter
(p.26)
17