Nikon eclipse lv150 Instruction Manual page 46

Detailed specification of excitation light balancer
Transmittance
The transmittance for the FITC is designed to keep approximately 100%, because the FITC is
usually dark fluorescent image.
Optical path position
Between (1) and (2)
Between (2) and (3)
44
(1)
(2)
A
100%
A
50%
B
DAPI
0%
350 400 450
(1)
Variable (100% to 50%)
(2)
Variable (50% to 0%)
(3)
B
(3)
B
A
TRITC
FITC
Texas-Red
500 550
DAPI FITC
100% 100%
100%
50% 100%
100%
0% 100%
III. Operation of Each Part
: Effective diameter
on the aperture
diaphragm surface
Wavelength
600
650 700
TRITC / Texas-Red
0%
Variable (0% to 50%)
50%
Variable (50% to 100%)
100%