Condenser - Olympus BX45 Instructions Manual

Aperture iris
diaphragm image
Objective pupil
@
2
Compatibility of Objectives and Condensers
* When using a 4X or lower-power objective, fully open the condenser aperture iris diaphragm and use the field iris
diaphragm in the base as aperture diaphragm. With the 1.25X to 2X objectives, the peripheral sections of the field of view
may be dark but observation is still possible.
}If you want objectives for cytological observation, please purchase the PlanN10XCY and PlanN series objectives or the
PlanFLN10XCY and UPlanFLN series objectives.
16
70-80%
30-20%
Fig. 26
²
N.A. scale
Fig. 27
Objective Magnification
1.25X - 4X *
4X -60X
100X
Aperture Iris Diaphragm (Figs. 26 & 27)
· The aperture iris diaphragm determines the numerical aperture of the
illumination system. Matching the numerical aperture of the illumination
system with that of the objective provides better image resolution and
contrast, and also increases the depth of focus.
· Since the contrast of microscope specimens is ordinarily low, setting the
condenser aperture iris diaphragm to between 70% and 80% of the N.A.
of the objective in use is usually recommended. If necessary, adjust the
ratio by removing the eyepiece and looking into the eyepiece sleeve
while adjusting the aperture iris diaphragm ring @ until the image shown
in Fig. 26 is seen.
}Using the numerical aperture scale:
Set the condenser numerical aperture scale to about 80% of the NA
value ² of the respective objective. (Fig. 27)
Example: With the UPlanFLN40X (NA 0.75), set the scale to 0.75 x 0.8 = 0.6.
BX45 Condenser
Applicable by swing the top lens out.
Applicable by engaging the top lens in the light path.