Product Overview - tecan RESOLVEX M10 96 Operating Manual

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Product Overview

The RESOLVEX™ M10 96 and RESOLVEX™ M10 96 XT (Extra Tall) for Positive Pressure
Solid Phase Extraction (SPE) offers state-of-the-art operation for SPE in 96-well formats.
The main difference between the standard M10 96 configuration and its M10 96 XT counterpart
is that in the XT model, the height travel range of the pressure manifold has been extended by
0.954" (2.42 cm.) to accommodate taller column stacks.
The configuration setup that the end-user intends to use, will dictate which M10 Processor to use.
Typically:

If you intend to use a 96-Place rack with regular-sized collection plates, you will need the
XT.
o These 96-Place racks are intended to be used with 95 or less columns.

All other users will need the standard M10 96 configuration
For simplicity, the rest of this operating manual will refer to both product configurations as the
RESOLVEX M10 96
Positive pressure processing of SPE columns exhibits a number of advantages compared to
traditional vacuum manifold processing.
One major problem with conventional vacuum manifolds occurs as faster flowing columns on the
manifold run dry, allowing a free flow path for vacuum through the dry columns, slowing the flow
on the remaining columns. This results in additional variation in column processing times, thus
contributing to irreproducible analyte recoveries. Also, when running fewer columns than needed
to accommodate all manifold ports, open ports must be plugged or switched off to prevent vacuum
loss, which otherwise causes insufficient flow through the sample columns.
In contrast to vacuum manifolds, the RESOLVEX™ M10 96 uses unique, restricted gas ports.
These ports cause gas flow control such that even if 95 processing channels are wide open, the
remaining single channel will receive appropriate pressure to allow facile processing. This feature
provides very uniform flow across all column channels, and eliminates the need for plugs of caps
when processing fewer than 96 samples.
When processing viscous samples, vacuum manifolds often cannot provide adequate processing
power since the maximum pressure is limited to something less than atmospheric (≈15 PSI/1.03
bar). The RESOLVEX™ M10 96 allows for gas pressures up to 5.5 bar (80 PSI), supplying
greater motive force for viscous sample flow. If the user adjusts the pressure up or down, the
column flow response is smooth and immediate.
The RESOLVEX™ M10 96 is self-adjusting to column height, allowing it to accommodate all
commercial 96-well plates, both modular and integrated, without the need for special adapters or
accessories.
Operating Manual for RESOLVEX
TM
M10 96 & M10 96 XT P/N 278-2036 Rev C Page 3/19