Thermo Scientific HM 450 Instruction Manual page 22

2-6-5 RETRACT FUNCTION FOR
COMPENSATING THE THERMAL
EXTENSION
Normally the ambient air slowly warms up the
paraffin specimen which was cooled down before.
Due to the rising temperature, the paraffin expands
and the achieved sections will be slightly thicker
than the pre-selected value.
Especially after longer breaks between the
sections, a thermal expansion becomes obvious
resulting in a thicker first cut at the same section
thickness setting. This thermal expansion of the
specimen can be compensated by using the retract
function.
•
Before starting cutting again, briefly press the
arrow-down-button of the coarse feed once.
•
The specimen will be moved by 2 µm
downwards away from the knife.
•
Now normal sectioning processes can be
started.
22
Thermo Scientific Sliding Microtome Microm HM 450
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 760 - English