Temperature Controls - Thermo Scientific RC 12BP Plus Instruction Manual

Temperature Controls

When verifying temperature control, all test run conditions (TEMP, SPEED, TIME, ROTOR and
option selections) should match your required protocol(s). Test fluid must be at the same volume and
temperature as the actual sample (use a calibrated thermometer to confirm). The sample temperature,
rotor assembly temperature, and SET temperature must match also. Ideally, all components would
have been stored in the same controlled-temperature area overnight. If that is not possible, you can
precool or preheat the rotor as required. Immediately after the test run is performed, check test fluid
temperature using the same thermometer that was used before the run.
1. Set the run parameters. Choose the protocol with the longest run time.
2. Equilibrate the rotor chamber and the temperature of all rotor parts (include any tubes, bottles, or
adapters to be used) to the desired sample temperature.
3. Prepare two equal loads of test fluid (similar in volume to normal run samples) equilibrated to the
desired sample temperature. Check the sample temperature using a calibrated thermometer.
Note Test fluid must be compatible with aluminum and have a freezing point below the desired
sample temperature.
4. Load and balance the rotor according to the instructions in the rotor manual.
5. Install the rotor in the chamber.
6. Close the chamber door.
7. Press START key.
During the run, the RUN display temperature should be within 2°C of the SET DISPLAY
temperature.
8. Immediately after the run measure the sample temperature using the same calibrated thermometer
used earlier.
The measured sample temperature should be within 2°C of SET temperature.
9. If required, repeat this procedure at any other speed/temperature ranges that are commonly used in
your run protocols.
10. If an out-of-range temperature is observed, re-equilibrate the chamber, rotor, and test fluid
temperatures to match desired sample temperature, and repeat the test run.
Thermo Scientific
5
Customer Control Inspection
Temperature Controls
RC 12BP+ 5-3