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Summary of Contents for Thermo Scientific RC 12BP Plus
- Page 1 Thermo Scientific RC 12BP Plus Instruction Manual 50125346-3 December 2010...
- Page 2 © 2010 Thermo Fisher Scientific Inc. All rights reserved. RC 12BP+ is either a registered trademark or a trademark of Thermo Fisher Scientific. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Fisher Scientific Robert-Bosch-Straße 1 D - 63505 Langenselbold Germany...
- Page 4 WEEE Conformity This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96 It is marked by the following symbol: Thermo Fisher Scientific has entered into agreements with recycling and disposal companies in all EU Member States for the recycling and disposal of this device.
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Page 5: Table Of Contents
Close Lid ......................... 4-2 Door Gas Springs 4- 3 Rotor Installation ..................... 4-3 Normal Operation ....................4-3 Centrifugation ......................4-5 Programmed Operation ................... 4-6 Storing Selections to Program Memory 4- 6 Performing a Programmed Run 4- 7 Thermo Scientific RC 12BP+... - Page 6 Autoclaving ......................6-5 Service of Thermo Fisher Scientific ................6-6 Chapter 7 Troubleshooting ........................7-1 Mechanical Emergency Door Release ..............7-2 Circuit Breakers ....................... 7-3 Failure message ......................7-3 Chemical Compatibility Chart ................A-1 Warranty ........................B-1 Contact Information ....................C-1 RC 12BP+ Thermo Scientific...
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Page 7: Preface
This symbol refers 3 phase alternating current. Scope of Supply Article Number Quantity Check Centrifuge RC 12BP+ Power supply cable 50105346 Instruction manual 70907249 Installation kit If any parts are missing, please contact your nearest Thermo Fisher Scientific representative. Thermo Scientific RC 12BP+... -
Page 8: Intended Use
• Use only rotors and accessories for this centrifuge which have been approved by Thermo Fisher Scientific. Exceptions to this rule are commercially available glass or plastic centrifuge tubes, provided they have been approved for the speed or the RCF value of the rotor. RC 12BP+ Thermo Scientific... - Page 9 When centrifuging microbiological samples from the Risk Group II (according to the Bio-safety Manual" of the World Health Organization WHO), aerosol-tight biological seals have to be used. For materials in a higher risk group, extra safety measures have to be taken. Thermo Scientific RC 12BP+...
- Page 10 CENTRIFUGE AND LEAVE THE AREA IMMEDIATELY. Normal use The centrifuge is to be used for separating materials of different density or particle size suspended in a liquid. Maximum sample density at maximum speed: 1,2 ---------- - RC 12BP+ Thermo Scientific...
- Page 11 “Characteristics of the RC 12BP+” page 1-2 • “Technical Data” page 1-3 • “Directives, Standards and Guidelines” page 1-4 • “Functions and Features” page 1-4 • “Mains Supply” page 1-5 • “Rotor Selection” page 1-5 • Thermo Scientific RC 12BP+...
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Page 12: Chapter 1 Introduction And Description
The centrifuge cannot be started until the lid has been closed properly. • Electronic imbalance detection. • Lid emergency release: For emergencies only, e.g. during power failures (see “Failure message” page 6-3) RC 12BP+ Thermo Scientific... -
Page 13: Technical Data
-10 °C to +40 °C Dimensions Height 1178 mm 46.3 inch Width 800 mm 31.5 inch Depth 905 mm 35.6 inch Length power cord 3000 mm 118.1 inch Weight without rotor 475 kg 1047 lb front side measurement Thermo Scientific RC 12BP+... -
Page 14: Directives, Standards And Guidelines
The most recent data is saved Functions RCF-selection, temperature control Acceleration / braking profiles 11 acceleration and 11 braking curves Imbalance detection Electronic, contingent on rotor and speed Lid lock Automatic lid closing and locking starting from an initial hold position RC 12BP+ Thermo Scientific... -
Page 15: Mains Supply
77080 The technical data of the rotors and the corresponding adapters and reduction sleeves for various commercially available containers can be found in the corresponding rotor operating manuals. For more information visit our website at: http://www.thermo.com Thermo Scientific RC 12BP+... - Page 16 2-2 • “Location” page 2-2 • “Installation” page 2-2 • “Aligning the Centrifuge” page 2-5 • “Transporting the Centrifuge” page 2-5 • “Mains Connection” page 2-5 • “Other connections” page 2-6 • “Storage” page 2-6 • Thermo Scientific RC 12BP+...
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Page 17: Chapter 2 Before Use
1. Place the pallet with the centrifuge so that you have at leased 2 m room in front of the pallet. 2. Release the rails from the pallet. 3. Screw the rails onto the pallet in front of the centrifuge. RC 12BP+ Thermo Scientific... - Page 18 6. Remove the four screws that fix the centrifuge t the pallet. The heads are upsiede down in the pallet. 7. Remove the bolt spacers. 8. Screw the studs with the safety brackets entirely up. Otherwise they might get stuck when rolling the centrifuge off the pallet. 9. Remove the rubber feet. Safety brackets Thermo Scientific RC 12BP+...
- Page 19 Mount the safety brackets by means of an 8 mm open-end wrench on the stabilizers. 22. Optional: To be certain the centrifuge is working properly, refer to the Customer Control Inspection paragraph located in “Customer Control Inspection” page 5-1. Perform the procedures listed under Speed Control, Timer, and Temperature Control. RC 12BP+ Thermo Scientific...
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Page 20: Aligning The Centrifuge
3. Check whether the cable complies with the safety standards of your country. 4. Make sure that the voltage and frequency correspond to the figures on the rating plate. 5. Establish the connection to the power supply with the connecting cable. Thermo Scientific RC 12BP+... -
Page 21: Other Connections
• Before storing the centrifuge and the accesories it must be cleanedand if necessary desinfected and decontaminated. • Store the centrifuge in a clean, dust-free location. • Be sure to place the centrifuge on its feet. • Avoid direct sunlight. RC 12BP+ Thermo Scientific... -
Page 22: Control Panel
Control Panel Contents “KEYS” page 3-2 • “CONTROLL PANEL” page 3-2 • “RUN DISPLAY” page 3-3 • “PRIMARY FUNCTION KEYS” page 3-4 • “SET DISPLAY” page 3-7 • “KEYPAD LOCK” page 3-8 • Thermo Scientific RC 12BP+... -
Page 23: Controll Panel
Press the SPEED key to enter the speed or RCF selection. Press HOLD key to select an continues run. Press TIME key to enter the time selection. Press ACE key to enter ACE value. Press TEMP key to enter the temperature. RC 12BP+ Thermo Scientific... -
Page 24: Run Display
Calculated ACE value ( ). When the set ACE value is reached, the run terminates and decelerates to a stop. The displayed value continues to calculate the centrifugal effect during deceleration to zero to show total accumulation. Thermo Scientific RC 12BP+... -
Page 25: Primary Function Keys
• An entered value will continue to flash (prompting input) when it is out of range for the SET rotor. The formula used to calculate RCF is: ⎛ ⎞ × × 11.18 radius in cm ----------- - ⎝ ⎠ 1000 RC 12BP+ Thermo Scientific... - Page 26 This variation can be attributed to rotor load/configuration differences (affecting inertia), fluctuations in line voltage (affecting motor power), or slight mechanical differences including normal wear. Termination Set Point TIME Thermo Scientific RC 12BP+...
- Page 27 • Always be sure of the control method and value before pressing START. The integral value can be calculated deriving the following formula: ∫ ∫ ω ω ω Δt Δt Where Change in time (seconds) ω Angular speed π n/60 ] Speed (rpm) RC 12BP+ Thermo Scientific...
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Page 28: Set Display
• If you have pressed MENU to access the options screens, pressing MENU again will return the set values to the SET DISPLAY. • The SET DISPLAY is the primary interactive screen on the control panel, and has a role in all aspects of centrifuge use. Thermo Scientific RC 12BP+... -
Page 29: Keypad Lock
ENTER and CLEAR, START and STOP are active). • Use of the KEYPAD LOCK may offer a considerable advantage, improving process control by reducing the possibility for operator error, as well as restricting unauthorized run parameter or program memory changes. RC 12BP+ Thermo Scientific... -
Page 30: Operation
“Rotor Installation” page 4-3 • “Normal Operation” page 4-3 • “Centrifugation” page 4-5 • “Programmed Operation” page 4-6 • “Using Advanced Features (Options)” page 4-8 • “Temperature Control” page 4-13 • “Rotor Temperature Equilibration” page 4-14 • Thermo Scientific RC 12BP+... -
Page 31: Switch On Centrifuge
Close the lid by pressing down on it lightly in the middle or on both sides of it. Two locks close the lid completely. The lid will close automatically. WARNING Do not reach into the crack between the lid and the housing. The lid is drawn shut automatically. Note The lid should audibly click into place. RC 12BP+ Thermo Scientific... -
Page 32: Door Gas Springs
2. Enter the desired value using the numeric pad. The digits show in sequential order. Note If you select a value that is to high for the defined rotor it will not be accepted. The value will continue to blink. Thermo Scientific RC 12BP+... - Page 33 Your entry will be automatically confirmed if you do not press any key for 5 seconds. HOLD Press the HOLD key in order to select the HOLD run. During HOLD, the centrifuge will continue running until you stop it manually with the STOP key. RC 12BP+ Thermo Scientific...
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Page 34: Centrifugation
You can also stop the centrifugation program manually at any time by pressing the STOP key. When you select an ACE controll run, the run will determine when the centrifuge has reached the set ACE value. Thermo Scientific RC 12BP+... -
Page 35: Programmed Operation
SET DISPLAY: 4. Press 1. The following screen will appear in the SET DISPLAY: Note Single-digit program numbers 1-9 are for storing sets of standard run parameters; double-digit program numbers 10-15 are for storing step-runs. RC 12BP+ Thermo Scientific... -
Page 36: Performing A Programmed Run
START key will be pressed. Note If you select a program number that has not been used to save a program, a warning message will be displayed. In this case no program will be recalled. Thermo Scientific RC 12BP+... -
Page 37: Using Advanced Features (Options)
Pressing 0 deselects options. Pressing ENTER steps through options without changing selections or settings, and on secondary screens, enters values to select the option. Pressing MENU exits the options and returns to the SET DISPLAY without entering/selecting a flashing value. RC 12BP+ Thermo Scientific... - Page 38 MENU 0 or ENTER ENTER MENU 0 or ENTER ENTER MENU 0 or ENTER ENTER MENU 0 or ENTER ENTER MENU 0 or ENTER ENTER MENU 0 or ENTER ENTER Figure 4-1. Advanced Feature Option Screens Thermo Scientific RC 12BP+...
- Page 39 The QC RUN sequence will begin, running program number 1. • If, during a QC RUN, the observed RUN temperature is more than 2°C over SET temperature, the rotor continues spinning until set temperature is reached. 4-10 RC 12BP+ Thermo Scientific...
- Page 40 500, transition to the specified SLOW STOP rate, then change to a coasting stop when the specified BRAKE OFF transition speed is reached. • After BRAKE OFF selections are completed, CHANGE OVERTEMPERATURE LIMIT? will appear in the SET DISPLAY. Thermo Scientific RC 12BP+ 4-11...
- Page 41 • Step runs can be saved to program memory (program numbers 10-15) for simple recall in future use (see SAVE RUN below). • Runs are not inadvertently corrupted, whenever STEP RUN has been selected (indicator lit). 4-12 RC 12BP+ Thermo Scientific...
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Page 42: Temperature Control
With such a run, if all components have been equilibrated, it would be difficult to significantly change sample temperature during the run - even if the centrifuge's temperature control performance was reduced. Thermo Scientific RC 12BP+ 4-13... -
Page 43: Rotor Temperature Equilibration
10. Monitor progress of the value in the RUN TEMPERATURE display, and note the time when the displayed RUN temperature reaches the SET temperature. From the time it does, allow the run to continue for an additional 30 minutes, then press STOP. 4-14 RC 12BP+ Thermo Scientific... -
Page 44: Customer Control Inspection
Customer Control Inspection Contents “Speed Controls” page 5-2 • “Timer Controls” page 5-2 • “Temperature Controls” page 5-3 • Thermo Scientific RC 12BP+... -
Page 45: Speed Controls
The stopwatch should read between 9:30 to 10:30, representing 10:00 % (29 seconds) plus an additional second to allow for cumulative human error. 8. If necessary, repeat this procedure at other time ranges that are commonly used in your protocols. RC 12BP+ Thermo Scientific... -
Page 46: Temperature Controls
9. If required, repeat this procedure at any other speed/temperature ranges that are commonly used in your run protocols. 10. If an out-of-range temperature is observed, re-equilibrate the chamber, rotor, and test fluid temperatures to match desired sample temperature, and repeat the test run. Thermo Scientific RC 12BP+... -
Page 47: Maintenance And Care
Maintenance and Care Contents “Cleaning Intervalls” page 6-2 • “Cleaning” page 6-2 • “Disinfection” page 6-4 • “Decontamination” page 6-5 • “Autoclaving” page 6-5 • “Service of Thermo Fisher Scientific” page 6-6 • Thermo Scientific RC 12BP+... -
Page 48: Cleaning Intervalls
• Store the aluminum parts at room temperature or in a cold-storage room with the cavities pointing down. CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. RC 12BP+ Thermo Scientific... - Page 49 To defrost the chamber: 1. Install a rotor and close the centrifuge. 2. Set run parameters to SPEED=2500 rpm, TIME=5:00 (longer for heavy frost buildup), TEMP=30. 3. Press the START key. Clean the rotor chamber as described above. Thermo Scientific RC 12BP+...
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Page 50: Disinfection
50°C. • After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (7000 9824). Also treat the cavities with oil. • Treat the bolts of the swing out rotor with bolt grease (75003786). RC 12BP+ Thermo Scientific... -
Page 51: Decontamination
• After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (7000 9824). Also treat the cavities with oil. • Treat the bolts of the swing out rotor with bolt grease (75003786). Autoclaving 1. Before autoclaving clean rotor and accessories and described above. Thermo Scientific RC 12BP+... -
Page 52: Service Of Thermo Fisher Scientific
• the suitability of the set-up site • the lid lock and the safety system • the rotor • the fixation of the rotor and the drive shaft Thermo Fisher Scientific offers inspection and service contracts for this work. RC 12BP+ Thermo Scientific... -
Page 53: Troubleshooting
Troubleshooting Contents “Mechanical Emergency Door Release” page 7-2 • “Circuit Breakers” page 7-3 • “Failure message” page 7-3 • Thermo Scientific RC 12BP+... -
Page 54: Mechanical Emergency Door Release
5. Pull down both levers on the right hand side. The door lock will be release mechanical. The lid will open and the samples can be removed. Levers Figure 7-1. Emergency door release 6. Close the door and lock it well. RC 12BP+ Thermo Scientific... -
Page 55: Circuit Breakers
The cooling circuit is open. ERROR The temperature is >45 °C. Wait for the rotor to cool down in order to prevent burn. Turn off the centrifuge. Pull out the power supply plug. Inform Customer Service. Thermo Scientific RC 12BP+... - Page 56 Restart the centrifuge. down without being braked. If an error message appears again, turn of the centrifuge. Pull out the power supply plug. The emergency lid release enables you to retrieve your samples. Inform Customer Service. RC 12BP+ Thermo Scientific...
- Page 57 Restart the centrifuge. down without being braked. If an error message appears again, turn of the centrifuge. The emergency lid release enables you to retrieve your samples. Pull out the power supply plug. Inform Customer Service. Thermo Scientific RC 12BP+...
- Page 58 Restart the centrifuge. down without being braked. If an error message appears again, turn of the centrifuge. The emergency lid release enables you to retrieve your samples. Pull out the power supply plug. Inform Customer Service. RC 12BP+ Thermo Scientific...
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Page 59: Chemical Compatibility Chart
S U U U U U U U - M S M - U S S M S S U S S S S S S S S S U S S S S S S S S S S Boric Acid Thermo Scientific RC 12BP+... - Page 60 S S S U S U S S S U Ethylene Oxide Vapor ® Ficoll-Hypaque M S S - S S S - S S S S - S S - S S S S S S S M S S S RC 12BP+ Thermo Scientific...
- Page 61 S S S U S S M S S S S S U S U M S S S S S S S M S M U Methyl Alcohol Methylene Chloride U U U U M S S U S U U S U U U U U M U U U S S M U S U Thermo Scientific RC 12BP+...
- Page 62 S S S S S S S S S S S S S S S S S S S Physiologic Media (Serum, Urine) Picric Acid S S U - S M S S S M S U S S S U S S S S U S U M S M S RC 12BP+ Thermo Scientific...
- Page 63 S S S S S S S S S S S S S S S S S S S S S S S Zinc Sulfate Citric Acid (10%) M S S M S S M S S S S S S S S S M S S S S S S S S S S Thermo Scientific RC 12BP+...
- Page 64 Performance unknown; suggest testing, using sample to avoid loss of valuable material. Chemical resistance data is included only as a guide to product use. Because no organized chemical resistance data exists for materials under the stress of centrifugation, when in doubt we recommend pretesting sample lots. RC 12BP+ Thermo Scientific...
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Page 65: Warranty
Thermo Fisher Scientific makes no warranty of any kind, expressed or implied, except as stated in this warranty policy. The Thermo Scientific RC 12BP+™ Centrifuge is warranted (subject to the conditions specified below and in the warranty clause of the Thermo Fisher Scientific terms and conditions of sale in effect at the time of sale) to be free from defects in material and workmanship for a period of one (1) year from the date of delivery. -
Page 66: Contact Information
08001 536 376 +49 6184 90 6940 France +33 2 2803 2180 Italy +39 02 02 95059 434-254-375 Netherlands +31 76 571 4440 Nordic / Baltic Countries +35 89 329 100 Russia/CIS +7 (812) 703 42 15 Thermo Scientific RC 12BP+... - Page 67 +86 21 6865 4588 +86 10 8419 3588 India 1800 22 8374 +91 22 6716 2200 Japan +81 45 453 9220 Other Asian Countries +852 2885 4613 Other Countries +49 6184 90 6940 +33 2 2803 2180 RC 12BP+ Thermo Scientific...
- Page 68 Failure message ..........7-3 Starting centrifuge program ......4-5 Stopping the centrifuging program ....4-5 Storage ............2-6 HOLD Run ............ 4-6 Transporting the Centrifuge ......2-5 Troubleshooting ..........7-1 Imbalance indicator ......... 4-5 Intended Purpose ..........vi Keys ..............3-2 Lid Opening ........... 4-2 Thermo Scientific RC 12BP+...
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