Subscribe to Our Youtube Channel
Related Manuals for Thermo Scientific RC 3BP Plus
Summary of Contents for Thermo Scientific RC 3BP Plus
- Page 1 Thermo Scientific Sorvall RC 3BP Plus Instruction Manual 50113587-6 04 / 2013 Visit us online to register your warranty. www.thermoscientific.com/labwarranty...
- Page 3 Thermo Scientific Sorvall RC 3BP Plus Instruction Manual 50113587-6 04 / 2013...
- Page 4 © 2013 Thermo Fisher Scientific Inc. All rights reserved. Delrin, TEFLON, and Viton are registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark of Hongye Chemical Co., Ltd. Hypaque is a registered trademark of Amersham Health As.
- Page 6 WEEE Conformity This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96. It is marked by the following symbol: Thermo Fisher Scientific has entered into agreements with recycling and disposal companies in all EU Member States for the recycling and disposal of this device.
-
Page 7: Table Of Contents
Accident Prevention .........................iv Precautions ..........................v Chapter 1 Introduction and Description ....................1-1 Characteristics of the Thermo Scientific Sorvall RC 3BP Plus ......... 1-2 Technical Data ......................1-3 Directives, Standards and Guidelines ................1-4 Functions and Features ......................1-4 Mains Supply ......................1-5 Rotor Selection ...................... - Page 8 Service of Thermo Fisher Scientific ................7-6 Chapter 8 Troubleshooting ........................8-1 Mechanical Emergency Door Release ..............8-2 Circuit Breakers ....................... 8-3 Failure message ......................8-3 Chemical Compatibility Chart ................A-1 Warranty ........................B-1 Contact Information ....................C-1 Sorvall RC 3BP Plus Thermo Scientific...
-
Page 9: Preface
This symbol refers to a rotor failure. This symbol refers 3 phase alternating current. Scope of Supply Article number Quantity Check Centrifuge Sorvall RC 3BP Plus Power supply cable 50127698 CD with instruction manual 70907249 Installation kit If any parts are missing, please contact your nearest Thermo Fisher Scientific representative. -
Page 10: Intended Use
The mains plug and main switch must be freely accessible at all times. Pull out the power supply plug or disconnect the power supply in an emergency. Sorvall RC 3BP Plus Thermo Scientific... -
Page 11: Precautions
• Never use the centrifuge if parts of its cover panels are damaged or missing. • Do not touch the electronic components of the centrifuge or alter any electronic or mechanical components. • Please observe the safety instructions. Thermo Scientific Sorvall RC 3BP Plus... - Page 12 Highly corrosive substances which can cause material damage and impair the mechanical stability of the rotor, should only be centrifuged in corresponding protective tubes. WARNING If a hazardious situation occurs, turn off the power supply to the centrifuge and leave the area immediately. Sorvall RC 3BP Plus Thermo Scientific...
- Page 13 Introduction and Description Contents • “Characteristics of the Thermo Scientific Sorvall RC 3BP Plus” page 1-2 “Technical Data” page 1-3 • • “Directives, Standards and Guidelines” page 1-4 “Functions and Features” page 1-4 • • “Mains Supply” page 1-5 “Rotor Selection”...
-
Page 14: Chapter 1 Introduction And Description
RCF or the entry mode. These settings can be changed even while the centrifuge is running. The Sorvall RC 3BP Plus is equipped with various safety features: • Housing, rotor chamber, baseframe, and guard ring are made of high-strength, high-quality steel. -
Page 15: Technical Data
1 Introduction and Description Technical Data Technical Data The technical data of the Sorvall RC 3BP Plus is listed in the following table. Table 1-1. Technical Data Feature Value Environmental conditions • Use in interior spaces • Altitudes of up to 2,000 m above sea level •... -
Page 16: Directives, Standards And Guidelines
Functions and Features The following table gives an overview of the important functional and performance characteristics of Sorvall RC 3BP Plus. Table 1-3. Functions and Features Component / Function Description / Features... -
Page 17: Mains Supply
3200 W 25 A & 6 A 3x32 A IEC60309 CEE 32 A-6h 1P+N+PE Rotor Selection The Sorvall RC 3BP Plus centrifuge is supplied without a rotor. Various Thermo Scientific rotors are available to choose from. H-6000A 11250 HBB-6 11753 HLR-6... -
Page 18: Before Use
“Before Setting up” page 2-2 “Location” page 2-2 • • “Setting up” page 2-2 “Transporting the Centrifuge” page 2-5 • • “Mains Connection” page 2-5 “Other Connections” page 2-5 • • “Storage” page 2-6 Thermo Scientific Sorvall RC 3BP Plus... -
Page 19: Before Setting Up
1. Place the pallet with the centrifuge so that you have at least 2 m room in front of the pallet. 2. Release the rails from the pallet. 3. Screw the rails onto the pallet in front of the centrifuge. Sorvall RC 3BP Plus Thermo Scientific... - Page 20 7. Remove the bolt spacers. 8. Screw the studs with the safety brackets entirely up. Otherwise they might get stuck when rolling the centrifuge off the pallet. 9. Remove the rubber feet. Safety brackets Studs Thermo Scientific Sorvall RC 3BP Plus...
- Page 21 CAUTION The horizontal alignment of the centrifuge must be checked every time after moving it to a different location. 22. Optional: To be certain the centrifuge is working properly, refer to the Customer Control Inspection paragraph located in “Customer Control Inspection” page 6-3. Sorvall RC 3BP Plus Thermo Scientific...
-
Page 22: Transporting The Centrifuge
3. Make sure that the voltage and frequency correspond to the figures on the rating plate. 4. Plug the centrifuge into grounded electrical sockets only. Other Connections 9 Pol D-Sub Connector Barcode Reader 15 Pol D-Sub Computer Inter- face Connector Figure 2-1. Connetors to external devises Thermo Scientific Sorvall RC 3BP Plus... -
Page 23: Storage
• Before storing the centrifuge and the accessories it must be cleaned and if necessary desinfected and decontaminated. • Store the centrifuge in a clean, dust-free location. • Be sure to place the centrifuge on its feet. • Avoid direct sunlight. Sorvall RC 3BP Plus Thermo Scientific... - Page 24 “Control Panel” page 3-2 “Numeric Keypad” page 3-2 • • “Run Display” page 3-2 “Set Display” page 3-3 • • “Primary Function Keys” page 3-3 “Primary Functions” page 3-4 • • “Keypad Lock” page 3-6 Thermo Scientific Sorvall RC 3BP Plus...
-
Page 25: Chapter 3 Control Panel
The toggles between positive and negative temperature values. Run Display The run display shows the actual values during a run. Speed field The speed field indicates either: Sorvall RC 3BP Plus Thermo Scientific... -
Page 26: Set Display
RCF selection. Press the key to select continuous run. Press the key in order to enter the time selection menu. Press the key to specify an ACE value. Press the key to specify a temperature. Thermo Scientific Sorvall RC 3BP Plus... -
Page 27: Primary Functions
The centrifuge translates set RCF values to rpm whole numbers for speed control purposes. The whole numbers are calculated to the closest RCF value. The speed control accuracy is not compromised, and the slight value difference will not affect your run. Sorvall RC 3BP Plus Thermo Scientific... - Page 28 • When ACE control is selected, the centrifuge calculates the effect of speed in relation to time (the shaded area in the illustration below). The run duration accounts for acceleration variation. Termination Set Point Running time Thermo Scientific Sorvall RC 3BP Plus...
-
Page 29: Keypad Lock
• The upper position, FULL (full function), allows use of all keys and features, without restriction; • The lower position, SINGLE (Run Single Program), minimizes functionality so that set parameters cannot be changed (the only active keys are Sorvall RC 3BP Plus Thermo Scientific... - Page 30 • Use of the KEYPAD LOCK may offer a considerable advantage, improving process control by reducing the possibility for operator error, as well as restricting unauthorized run parameter or program memory changes. Thermo Scientific Sorvall RC 3BP Plus...
-
Page 31: Operation
“Switch on Centrifuge” page 4-2 “Rotor Installation” page 4-3 • • “Normal Operation” page 4-3 “Centrifugation” page 4-5 • • “Programmed Operation” page 4-6 “Temperature Control” page 4-7 • • “Rotor Temperature Equilibration” page 4-7 Thermo Scientific Sorvall RC 3BP Plus... -
Page 32: Switch On Centrifuge
The door will close automatically. WARNING Do not reach into the crack between the door and the housing. The door is drawn shut automatically. Note The door should audibly click into place. Sorvall RC 3BP Plus Thermo Scientific... -
Page 33: Rotor Installation
2. Enter the desired value using the numeric pad. The digits show in sequential order. Note If you select a value that is to high for the defined rotor it will not be accepted. The value will continue to blink. Thermo Scientific Sorvall RC 3BP Plus... - Page 34 HOLD run. During continuous operation, the centrifuge will continue running until you stop it manually with the key. Preselecting the temperature You can preselect temperatures between -10° C and +40° C. 1. Press the key in order to enter the temperature. Sorvall RC 3BP Plus Thermo Scientific...
-
Page 35: Centrifugation
Press the key in the control panel. The centrifuge will be decelerated at the designated rate. The message will illuminate, and after pressing the key, the door will open and you can remove the samples. Thermo Scientific Sorvall RC 3BP Plus... -
Page 36: Programmed Operation
The SET DISPLAY will change to display a program number flashing in the PROG # field. 2. Input the desired program number, then press key. The SET DISPLAY and options indicators change to reflect the run parameters that were saved to that program number. Sorvall RC 3BP Plus Thermo Scientific... -
Page 37: Temperature Control
4. Check to be sure the correct rotor name is in the ROTOR field of the SET DISPLAY. If not, press key until it is. 5. Press the key. If cooling, input 1500 rpm. Thermo Scientific Sorvall RC 3BP Plus... - Page 38 10. Monitor progress of the value in the RUN TEMPERATURE display. Note the time when the displayed RUN temperature reaches the SET temperature. From the time it does, allow the run to continue for additional 30 minutes, then press the key. Sorvall RC 3BP Plus Thermo Scientific...
-
Page 39: Advanced Features
5-4 “SLOW START” page 5-4 • • “SLOW STOP” page 5-5 “BRAKE OFF” page 5-5 • • “CHANGE OVERTEMPERATURE LIMIT” page 5-5 “STEP RUN” page 5-6 • • “SAVE TO PROGRAM MEMORY?” page 5-7 Thermo Scientific Sorvall RC 3BP Plus... - Page 40 In the secondary screens you must enter a value to select the option. • Press the key to exit the options and return to the set display without entering/selecting a flashing value. Sorvall RC 3BP Plus Thermo Scientific...
- Page 41 0 or ENTER ENTER MENU 0 or ENTER ENTER MENU 0 or ENTER ENTER MENU 0 or ENTER ENTER MENU 0 or ENTER ENTER MENU 0 or ENTER ENTER Figure 5-1. Advanced Feature Option Screens Thermo Scientific Sorvall RC 3BP Plus...
- Page 42 SLOW START • SLOW START chooses gentle acceleration from 0 to 250 rpm. At 250 rpm the centrifuge accelerates at normal, maximum rate. SLOW START offers ten different acceleration rates. Sorvall RC 3BP Plus Thermo Scientific...
- Page 43 • The centrifuge calculates the difference between the set and the maximum temperatures. This value is used as an offset to apply to future runs, until it is changed. The retained offset will apply to any normal (manual entry) run. Thermo Scientific Sorvall RC 3BP Plus...
- Page 44 • The only changes that could be made without deselecting would be if a different step run was recalled from program memory (program numbers 10-15). • After STEP RUN selections are completed, SAVE TO PROGRAM MEMORY? will appear in the set display. Sorvall RC 3BP Plus Thermo Scientific...
- Page 45 • To save a run, all run parameter selections should be made before selecting this option. • After SAVE RUN selection and assignment of a program number, the set display will return to the home screen. Thermo Scientific Sorvall RC 3BP Plus...
-
Page 46: Control Inspection
Control Inspection Contents • “QC RUN” page 6-2 “Speed Controls” page 6-3 • • “Timer Controls” page 6-3 “Temperature Controls” page 6-4 • Thermo Scientific Sorvall RC 3BP Plus... -
Page 47: Qc Run
The QC RUN sequence will begin, running program number 1. Note If, during a QC RUN, the observed RUN temperature is more than 2° C over SET temperature, the rotor continues spinning until set temperature is reached. Sorvall RC 3BP Plus Thermo Scientific... -
Page 48: Customer Control Inspection
RUN TIME display counts down to 00:00. The stopwatch should read between 9:30 to 10:30, representing 10:00 % (29 seconds). 8. If necessary, repeat this procedure at other speeds that are commonly used in your protocols. Thermo Scientific Sorvall RC 3BP Plus... - Page 49 9. If necessary, repeat this procedure at other speeds that are commonly used in your protocols. 10. If an out-of-range temperature is observed, repeat the test run. Re-equilibrate the chamber, rotor, and test fluid temperatures to match desired sample temperature, and repeat the test run. Sorvall RC 3BP Plus Thermo Scientific...
- Page 50 Maintenance and Care Contents • “Cleaning Intervals” page 7-2 “Cleaning” page 7-2 • • “Disinfection” page 7-4 “Decontamination” page 7-5 • • “Autoclaving the rotors” page 7-6 “Service of Thermo Fisher Scientific” page 7-6 • Thermo Scientific Sorvall RC 3BP Plus...
-
Page 51: Chapter 7 Maintenance And Care
• Store the aluminum parts at room temperature or in a cold-storage room with the cavities pointing down. CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. Sorvall RC 3BP Plus Thermo Scientific... - Page 52 1. Install a rotor and close the centrifuge. 2. Set run parameters to SPEED = 2500 rpm, TIME = 5:00 (longer for heavy frost buildup), TEMP = 30. 3. Press the key. Clean the rotor chamber as described above. Thermo Scientific Sorvall RC 3BP Plus...
-
Page 53: Disinfection
• After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (70009824). Also treat the cavities with oil. • Tread the bolt of the swing out rotor with bolt grease (75003786). Sorvall RC 3BP Plus Thermo Scientific... -
Page 54: Decontamination
• After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (70009824). Also treat the cavities with oil. • Tread the bolt of the swing out rotor with bolt grease (75003786). Thermo Scientific Sorvall RC 3BP Plus... -
Page 55: Autoclaving The Rotors
• the suitability of the set-up site • the lock and the safety system • the rotor • the fixation of the rotor and the drive shaft Thermo Fisher Scientific offers inspection and service contracts for this work. Sorvall RC 3BP Plus Thermo Scientific... -
Page 56: Troubleshooting
Troubleshooting Contents • “Mechanical Emergency Door Release” page 8-2 “Circuit Breakers” page 8-3 • • “Failure message” page 8-3 Thermo Scientific Sorvall RC 3BP Plus... -
Page 57: Mechanical Emergency Door Release
Figure 8-1. Emergency door release 6. Close the front door and lock it well. Reconnect the centrifuge once the power has been restored. Switch on the centrifuge. Press the to have the door locks operative again. Sorvall RC 3BP Plus Thermo Scientific... -
Page 58: Circuit Breakers
The cooling circuit is open. MEASURE ERROR The temperature is >45° C. Wait for the rotor to cool down in order to prevent burn. Turn off the centrifuge. Pull out the power supply plug. Inform Customer Service. Thermo Scientific Sorvall RC 3BP Plus... - Page 59 If an error message appears again, turn off the centrifuge. Pull out the power supply plug. The emergency lid release enables you to retrieve your samples. Inform Customer Service. Sorvall RC 3BP Plus Thermo Scientific...
- Page 60 Restart the centrifuge. If an error message appears again, turn off the centrifuge. The emergency lid release enables you to retrieve your samples. Pull out the power supply plug. Inform Customer Service. Thermo Scientific Sorvall RC 3BP Plus...
- Page 61 Restart the centrifuge. If an error message appears again, turn off the centrifuge. The emergency lid release enables you to retrieve your samples. Pull out the power supply plug. Inform Customer Service. Sorvall RC 3BP Plus Thermo Scientific...
-
Page 62: Chemical Compatibility Chart
Ammoniumhydroxid (28%) Ammonium Hydroxide (conc.) Ammonium Phosphate Ammonium Sulfate Amyl Alcohol Aniline Sodium Hydroxide (<1%) Sodium Hydroxide (10%) Barium Salts Benzene Benzyl Alcohol Boric Acid Cesium Acetate Cesium Bromide Cesium Chloride Cesium Formate Cesium Iodide Thermo Scientific Sorvall RC 3BP Plus... - Page 63 Ethyl Alcohol (95%) Ethylene Dichloride Ethylene Glycol Ethylene Oxide Vapor Ficoll-Hypaque Hydrofluoric Acid (10%) Hydrofluoric Acid (50%) Hydrofluoric Acid (conc.) Formaldehyde (40%) Glutaraldehyde Glycerol Guanidine Hydrochloride Haemo-Sol Hexane Isobutyl Alcohol Isopropyl Alcohol Iodoacetic Acid Sorvall RC 3BP Plus Thermo Scientific...
- Page 64 Lactic Acid (20%) N-Butyl Alcohol N-Butyl Phthalate N, N-Dimethylformamide Sodium Borate Sodium Bromide Sodium Carbonate (2%) Sodium Dodecyl Sulfate Sodium Hypochlorite (5%) Sodium Iodide Sodium Nitrate Sodium Sulfate Sodium Sulfide Sodium Sulfite Nickel Salts Oils (Petroleum) Thermo Scientific Sorvall RC 3BP Plus...
- Page 65 Hydrochloric Acid (10%) Hydrochloric Acid (50%) Sulfuric Acid (10%) Sulfuric Acid (50%) Schwefelsäure (konz.) Stearic Acid Tetrahydrofuran Toluene Trichloroacetic Acid Trichloroethane Trichloroethylene Trisodium Phosphate Tris Buffer (neutral pH) Triton X-100 Urea Hydrogen Peroxide (10%) Sorvall RC 3BP Plus Thermo Scientific...
- Page 66 Performance unknown; suggest testing, using sample to avoid loss of valuable material. Chemical resistance data is included only as a guide to product use. No organized chemical resistance data exists for materials under the stress of centrifugation. When in doubt we recommend pretesting sample lots. Thermo Scientific Sorvall RC 3BP Plus...
-
Page 67: Warranty
Thermo Fisher Scientific makes no warranty of any kind, expressed or implied, except as stated in this warranty policy. The Thermo Scientific Sorvall RC 3BP Plus Centrifuge is warranted (subject to the conditions specified below and in the warranty clause of the Thermo Fisher Scientific terms and conditions of sale in effect at the time of sale) to be free from defects in material and workmanship for a period of one (1) year from the date of delivery. -
Page 68: Contact Information
+91 22 6716 2200 Japan +81 45 453 9220 Other Asian Countries +852 2885 4613 Australia +61 39757 4300 New Zealand +64 9 980 6700 Countries not listed +49 6184 90 6000 +33 2 2803 2180 Thermo Scientific Sorvall RC 3BP Plus... - Page 69 Stopping the centrifugation program ....4-5 Storage ............2-6 Failure messages ..........8-3 Transporting the Centrifuge ......2-5 Troubleshooting ..........8-1 HOLD ............4-5 Imbalance indicator ......... 4-5 Location ............2-2 Mains Connections ......... 2-5 Maintenace ............. 7-1 Thermo Scientific Sorvall RC 3BP Plus...
Need help?
Do you have a question about the RC 3BP Plus and is the answer not in the manual?
Questions and answers