Safety Concept Use in clean rooms Servicing Before using your macroscope for the first time, The Leica Z series can be used in clean rooms Repairs may only be carried out by Leica ★ please read the "Safety Concept" brochure without any problems.
This User Manual describes the special func- To maintain the unit in its original condition tions of the individual modules of the Leica Z and to ensure safe operation, the user must follow the instructions and warnings contained...
Failure to comply can cause the following: Figures Hazards to personnel ★ (1) Numbers in parentheses within the descriptions relate to the figures and the Functional disturbances or damaged inst- ★ items within those figures. ruments Leica Z6 APO A / Z16 APO A User Manual...
Touching the live circuit can cause injury. ★ conjunction with non-Leica components that are outside of the scope of this manual. Leica Z6 APO A / Z16 APO A User Manual...
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Disposal onal aspects. ★ Refer to "Safety Concept" booklet. The ergonomic design and construction of the Leica Z series are intended to reduce the exer- Legal requirements tion of the user to a minimum. Refer to "Safety Concept" booklet. ★...
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Before opening the lamp housing ★ When replacing the high-pressure mercury ★ lamp and other parts, such as the heat- absorbing filter or the collector During maintenance work on the supply ★ unit Leica Z6 APO A / Z16 APO A User Manual...
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To minimize the risk of explosion, replace a ★ discolored mercury lamp promptly Leica Microsystems assumes no liability ★ for damage caused by exploding, incor- rectly installed or improperly used mercury lamps. Leica Z6 APO A / Z16 APO A User Manual...
On We Go Leica LED3000 NVI™ (Near Vertical Illumination) Assembly Quick Start Guide Incident-light Bases and Manual Focusing Column Overview of an automated Leica Z Series Macroscope (Leica Z16 APO A) Transmitted-light Bases and Manual Focusing Column Microscope Carrier Eyepieces Video/Phototube A Video/Phototube AS ("Slim")
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Leica TL RC™ / TL RCI™: Operation Adapters Leica TL RCI™: Methods in Transmitted Light Coaxial incident-light housing Leica TL RCI™: Relief Images Using Filters Leica IsoPro™ (Non-motorized): Controls Leica IsoPro™ (Motorized): Controls Leica Z6 APO A / Z16 APO A User Manual...
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Specifications Leica Z6 APO A & Z16 APO A Optical Accessories for Leica Z6 APO A & Z16 APO A Ambient Conditions and Weights Optical Data – Visual with Y Tube 1.25× Leica TL ST Transmitted-light Base Leica TL BFDF Transmitted-light Base Leica TL RC™...
Though the reliability and robustness of Leica macroscopes is legendary, convinced it will exceed your expectations, as the Z series embodies all like any high-tech product, the Leica Z series requires a certain degree of the qualities you associate with the name Leica Microsystems: excellent care and attention.
The optics carriers, eyepieces, bases that cannot be met with standard parts, contact and more can be combined in any way you your Leica consultant. He or she has a solution choose, allowing you to create the macroscope for every problem.
To automate your workflows even more, also For the Leica Z6 APO A and Z16 APO A auto- saved and restored at the touch of a button. integrate the Leica IsoPro™ motorized XY stage mated macroscopes, motorized control of the –...
Leica macroscope yourself, continue with the consultant, click here to skip through the instal- "Assembly" chapter, which begins on Page 17. lation instructions and go directly to the Quick Start Guide on page 45. Leica Z6 APO A / Z16 APO A User Manual...
Microscope Carrier Tools used Assembling the Microscope Carrier Allen key provided 1. Press the microscope carrier against the 2. Tighten the screw using the Allen key provi- ★ focusing column. ded. Leica Z6 APO A / Z16 APO A User Manual...
1. Unscrew the locking screw on the optics carrier. 3. Tighten the locking screw on the micro- scope carrier. 2. Insert the video objective into the video/ phototube A and turn the knurled ring until tight. Leica Z6 APO A / Z16 APO A User Manual...
OEM applications and is usually not used on Leica focusing columns. After installation on the OEM equipment, the rest of the components are installed as described on the following pages. Leica Z6 APO A / Z16 APO A User Manual...
Assembling the tube lar tubes, ErgoModules®, video/phototu- scope carrier. 1. Insert the desired tube and turn the knurled bes and the Leica IC A video module of the Leica ring until tight (refer also to page 27). M series. Assembling the tube 1.
The coaxial illuminator is fitted between the optics carrier and the tube. It enables you to connect the light guide, which, in turn, is connected to a cold light source of the Leica CLS series. At low magnifications, using the coaxial...
(see page 24). In this case, the optics carrier is attached to the coaxial illuminator instead of the tube.Assembling the optics carrier Leica Z6 APO A / Z16 APO A User Manual...
HR and DM objectives are high-perfor- mance objectives and always cause vignetting. This vignetting is not a malfunction. Leica Z6 APO A / Z16 APO A User Manual...
When using the ergo binocular tube or another tube, the Y video/phototube must be used. Preparations 1. Unscrew the positioning screw and remove the protective cover.Assembling the tube Leica Z6 APO A / Z16 APO A User Manual...
2. Remove the plastic tube guard. You can extend the overall magnification range using available 10×, 16×, 25× and 40× wide-field eyepieces for persons wearing glasses. 4. Securely tighten the clamping screws. Leica Z6 APO A / Z16 APO A User Manual...
Make sure the instruments are unpa- using the Allen key provided. 3. Re-attach the adapter plate to its origi- cked on a flat, sufficiently dimensioned, and nal position using the six hexagon-head non-slip surface. screws. Leica Z6 APO A / Z16 APO A User Manual...
Removing the transport anchors Before you can use the transmitted-light base for the first time, it is absolutely necessary to remove the two transport anchors. Anchor of mirror Anchor of switching slide Leica Z6 APO A / Z16 APO A User Manual...
4. Insert the glass plate back into the standard stage. ★ Leica IsoPro™ manual mechanical stage Leica IsoPro™ automatic mechanical stage ★ Leica standard stage 10 447 269 ★ Leica Z6 APO A / Z16 APO A User Manual...
The following paragraph assumes use of the base without the stage mounted. Disassembly is performed in reverse order of the following steps. Leica Z6 APO A / Z16 APO A User Manual...
2. Turn the mechanical stage around and place it onto a non-slip surface. 3. Change the gear rod from the left to the right-hand side. 4. Skip the next two steps to install the cont- rols. Leica Z6 APO A / Z16 APO A User Manual...
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2. Change the gear rod from the left to the 4. Attach the cover rail to the mechanical will go in the direction of the column. right-hand side. stage. 5. Insert the glass plate into the mechanical stage. Leica Z6 APO A / Z16 APO A User Manual...
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Allen key provided. 2. Attach your focusing drive column from below using the three hexagon-head screws. 3. Re-attach the adapter plate to its original position using the three hexagon-head screws. Leica Z6 APO A / Z16 APO A User Manual...
The motorized mechanical stage is a sensitive precision instrument. During installation, avoid subjecting the stage to impact or severe vibra- tions. Microscope base 3× M4 screws Motorized mechanical stage Leica Z6 APO A / Z16 APO A User Manual...
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After removal, keep all the transport anchors in the plastic bag provided for future transport. Leica Z6 APO A / Z16 APO A User Manual...
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4. Now move the mechanical stage as far as it will go in the direction of the column. 5. Insert the glass plate into the mechanical stage. Leica Z6 APO A / Z16 APO A User Manual...
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CTL2 initializes and automatically drives to the mid interfaces. position. 2. Plug the CTL2 plug of the Leica PSC control- 4. Connect the PC (where applicable) and X/Y ler into another CTL2 interface. Stage DCI Module (using a suitable USB ★...
Cables: Connections The A version of the Leica Z series features The terminals The connection to the PC and to other instru- extensive automation options with which vari- ments is made using the terminals on the rear ous microscope data and settings can be read side of the column: out, transferred to the PC and reproduced later.
2. Remove the cover of the cable duct. Tip: Estimate the length of the cable ends you will need before screwing on the cover. For thick cables, it is difficult to change the length retroactively. Leica Z6 APO A / Z16 APO A User Manual...
Exception: If the power fails while the motorized focus is moving, the position data are lost. In this case, the calibration must be repeated using the Leica LAS software or the Leica SmartTouch™. To do so, please consult the respective manual.
CTL2-Ports Z6 / Z16 APO A Powersupply SmartTouch Footswitch*** ™ 10 450 266 Serial connection loop possible Adapter-cable 10 447 401 needed Serial connection of up to 5 footswitches possible Leica Z6 APO A / Z16 APO A User Manual...
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Power Supply Serial connection loop possible Serial connection of up to 5 footswitches possible For motorfocus only **** Not with new motorfocus SmartTouch Footswitch** Handwheel*** UMC**** ™ 10 450 298 Leica Z6 APO A / Z16 APO A User Manual...
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Power Supply CTL2 CTL2 Alternative installation Please use the Leica Application Suite to configure the functions of the system. LED3000 NVI SmartTouch Smart Move Connector from ™ IsoPro Stage ™ Leica Z6 APO A / Z16 APO A User Manual...
The Leica LED 3000 NVI™ can be used 2. Place the Leica LED3000 NVI™ on the objec- grated electronics. with any objectives that have an outer tive from below and tighten the locking diameter of 58 mm.
Overview of an automated Leica Z Series Macroscope (Leica Z16 APO A) Digital camera Photo/videotube Eyepieces Video/Phototube Y Microscope carrier Connection for control cable Optics carrier (zoom body) Objective LED ring illuminator 10 IsoPro™ motorized mechanical stage 11 SmartTouch control unit...
(see page 53). The soft material of the eyecup also ensures that your glasses will not be scratched, even if they contact the eyepiece. Leica Z6 APO A / Z16 APO A User Manual...
Not to worry — after a little while, it will become automatic. Reference value The distance between eye and eyepiece measu- ✓ ✗ res approx. 22 mm for 10/23B wide-field eyepi- eces for persons wearing glasses. Leica Z6 APO A / Z16 APO A User Manual...
5. Then, open the other eye and correct the diopter settings until the image is uniformly sharp. 6. Select the highest magnification and refo- cus if necessary. Leica Z6 APO A / Z16 APO A User Manual...
1. Screw the insert off of the eyepiece. 3. Screw the insert and graticule firmly into place and replace the eyepiece in the tube. Leica Z6 APO A / Z16 APO A User Manual...
Eyecups can be ordered separately. Please contact your Leica partner. Separate eyecups are an effective way of preventing infections. Leica Z6 APO A / Z16 APO A User Manual...
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Photography & Video Leica Z6 APO A / Z16 APO A User Manual...
The "Leica Application Suite", or "LAS" for short, work. Research results can be presented in an the camera and need the capability for measu- is, as it were, the digital extension of the Leica attractive manner; measurements on the digi- rement, evaluation and more in addition to Z series macroscopes.
Cameras from third-party suppliers All Leica DFC cameras are equipped with a ter with higher magnification. This primarily In addition to Leica DFC cameras with the stan- standardized C-mount interface. In turn, the avoids the critical border area of the field of...
50% of the light is diverted to the video/ ★ photo beam path. Assembly Fasten the "trinocular tube 50%" to the optics carrier instead of the ErgoTube (refer also to ™ page 27). Leica Z6 APO A / Z16 APO A User Manual...
Assembly You can now photograph the specimen. Fasten the "trinocular tube 100%" to the optics carrier instead of the binocular ErgoTube (refer also to page 27). Leica Z6 APO A / Z16 APO A User Manual...
0.32× achromatic objectives, which can be attached to the zoom or the fine focusing using an adapter.You can attach the vertical illuminator or the attachment for incident/ oblique observation to the achromatic objective. Leica Z6 APO A / Z16 APO A User Manual...
Screws for changing the halogen lamp Power connection socket Adapter plate for easy assembly of focusing Power switch drives Removable glass plate Controller for light intensity Adjustment for deflection mirror Leica Z6 APO A / Z16 APO A User Manual...
Transmitted light that traverses the object obli- quely will provide additional resolution and information when observing semitransparent, opaque objects. Slowly pull the slider towards yourself until ★ the desired effect is achieved. Leica Z6 APO A / Z16 APO A User Manual...
2. Carefully pull out the lamp and mount by pulling them upwards. 3. Disconnect the lamp from the mount. 4. Insert the new lamp into the mount and reinsert the lamp holder. Leica Z6 APO A / Z16 APO A User Manual...
Button to toggle between bright field and dark field Button to toggle between bright field and dark Connector for cold light sources field (light conductor active f = 10mm, end tube f = 13mm) Leica Z6 APO A / Z16 APO A User Manual...
The Leica TL BFDF transmitted-light base has a control that switches the light from "bright field" to "dark field". Fingertip with bright field illumination Identical subject with dark field illumination Leica Z6 APO A / Z16 APO A User Manual...
Standard stage 10 447 269 Screws for changing the halogen lamp Filter holder Control of top and bottom flaps of the Rottermann Contrast™ Button for mirror and horizontal movement of the mirror Transmitted-light base Leica Z6 APO A / Z16 APO A User Manual...
This means that the lower switch, rather than illumination and dark field-like illumination is the upper switch, controls the inverted relief possible. contrast and vice-versa. Leica Z6 APO A / Z16 APO A User Manual...
To enter or exit dimming mode, click and ★ (Constant Color Intensity Control) hold down the scroll wheel for longer than illumination intensity 2 seconds. Potentiometer for controlling the color temperature Leica Z6 APO A / Z16 APO A User Manual...
Switch for adjusting the inverted relief cont- rast Switch for adjusting the positive relief cont- rast Deflection mirror Leica Z6 APO A / Z16 APO A User Manual...
A dark field-like transmitted light is created. Outlines, fine edges and structures are bright, in contrast with the dark background, through diffraction of the light beams on the dark background. Leica Z6 APO A / Z16 APO A User Manual...
2. Turn the deflection mirror into the notch gently tilting the deflection mirror. position at an angle of 45°. The effect can be strengthened or weakened by gently tilting the deflection mirror. Leica Z6 APO A / Z16 APO A User Manual...
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The dynamic effect makes it easy We recommend using the transmitted-light to distinguish phase structures from amplitude base with 1× or higher objectives, and not structures. objectives with a long focal length. Leica Z6 APO A / Z16 APO A User Manual...
Daylight filter for Leica TL ST The transmitted-light bases TL RC™ and TL RCI™ slot in the filter holder. A daylight filter is also available for the Leica TL can be equipped with up to three filters— ST transmitted-light base.
Operating the Leica IsoPro™ mechanical stage 1. To move the stage in the X direction, rotate the outer knob. 2. To move the stage in the Y direction, rotate the inner control ring. Leica Z6 APO A / Z16 APO A User Manual...
3 CTL2 interfaces Fine control in X direction Fine control in Y direction USB interface (type B) Leica IsoPro ™ motorized mechanical stage Socket for grounded power cable Housing with motorization Leica Z6 APO A / Z16 APO A User Manual...
The supported working distance is between 50 mm and 150 mm. 3. Switch off the illuminator by briefly pres- sing the button. The green LED on the upper left corner now lights up. Leica Z6 APO A / Z16 APO A User Manual...
The motorized Leica IsoPro™ mechanical stage offers an accuracy of up to 0.25µm. To move to a position with maximum accuracy, use the left and right knobs on the joystick. Leica Z6 APO A / Z16 APO A User Manual...
For additional information about the Leica SmartTouch and operation, refer to the User Manual provided with the instrument. Leica Z6 APO A / Z16 APO A User Manual...
2 short beeps – pause – 2 long beeps. Switching off the system cancels all of the ★ stored focus positions. When the system is switched on, the coarse focus is always switched on also. Leica Z6 APO A / Z16 APO A User Manual...
Footswitch Focusing Toggling between coarse and fine focus Press the left and right switches simultane- ously. Focus positions cannot be stored using the footswitch. Upwards Downwards Leica Z6 APO A / Z16 APO A User Manual...
Focusing arms Highest Focusing Positio Lowest Focusing Position Ø76 Interface for Ø25 Column Focusing arm for bonders Tiltable focusing arm Leica Z6 APO A / Z16 APO A User Manual...
Adapters ∅ 45 mm (Fine Focus Interface) ∅ 40 mm 6 mm 11 mm 8 mm (Achromat Objective Interface) ∅ 66 mm Adapter for achromatic objectives Adapter for DM objectives Leica Z6 APO A / Z16 APO A User Manual...
0.034 – 0.224 nA Object field ∅ 4.2 mm – 0.67 mm 4.2 mm – 0.26 mm Data with Leica DFC490 digital camera / 1× planapochromatic objective / AS tube / 0.63× video objective Magnification Chip: Object 0.36× – 2.3×...
Optical Accessories for Leica Z6 APO A & Z16 APO A Leica Z6 APO A & Z16 APO A Objectives • Planapochromat 1×, 2×, 0.8×, 0.5×, 5× • M series achromatic objectives 0.63×, 0.5×, 0.32×, lead-free 0.5×, 0.32×, lead-free Working distances • 187 mm (0.5× planapochromat) • 97 mm (1× planapochromat) • 112 mm (0.8× planapochromat) • 39 mm (2× planapochromat) • 19 mm (5.0× planapochromat) Objective adapters • For M series achromatic objectives • For 10× and 20× HR objectives...
Ambient Conditions and Weights Ambient operating conditions Weight Ambient temperature +10 ºC to +40 ºC 10 447 174 Leica Z6 APO A zoom system 1.030 kg Rel. humidity Up to 35 °C ambient temperature: 75% 10 447173 Leica Z16 APO A zoom system 1.330 kg Air pressure 700 –...
CCIC (Constant Color Intensity Control) Internal shutter/lamp control Integrated filter holder Coated optics for increasing the color temperature Matching of high num. aperture Remote control options AntiShock™ Pads Dimensions (W×H×D) 340×430×85 mm Leica Z6 APO A / Z16 APO A User Manual...
Matching of high num. aperture Yes** Remote control options Yes*** AntiShock™ Pads Dimensions (W×H×D) 340×390×90 mm *With cold light source Leica CLS150 LS **Concave mirror *** With external light source Leica Z6 APO A / Z16 APO A User Manual...
Yes*** Yes*** Remote control options Yes**** AntiShock™ Pads Dimensions of base (W×H×D) 340×390×95 mm 340×440×95 mm *Single-sided **With cold light source Leica CLS150 LS ***Concave mirror ****With external light source Leica Z6 APO A / Z16 APO A User Manual...
Protect your macroscope from oil and ★ and manufacture. It does not, however, cover grease. damage resulting from careless or improper handling. Do not grease guide surfaces or mechanical ★ parts. Leica Z6 APO A / Z16 APO A User Manual...
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Remove dust with a pneumatic rubber bulb ★ ★ not in use. or with a soft brush. Clean objectives and eyepieces with special ★ optic cleaning cloths and with pure alcohol. Leica Z6 APO A / Z16 APO A User Manual...