Preparing For Operation; Software And Data System; Calibrating And Tuning The Instrument - Waters ACQUITY RDa Overview And Maintenance Manual

2 Preparing for operation

To confirm that the instrument is ready for operation, verify the following conditions:
• The power supply, the vacuum pump, and the nitrogen gas are connected to the instrument.
See Appendix D (Page 88)
• The following fluidics connections are made:
• The LC outlet flow (from the column or optical detector) is connected to the divert valve.
• The ESI capillary is connected between the divert valve and ESI source inlet.
• The wash, lock mass, and calibration bottles all contain a sufficient volume of solution and
are mounted to the instrument in the correct reservoir slots. See
19).
• The instrument door is closed.
If you moved the instrument and you need to reconnect it, see
Operation. (Page 78)

2.1 Software and data system

UNIFI software, which controls the ACQUITY RDa Detector and LC system, enables the
following major operations:
• Configuring the system
• Using the setup feature to automatically tune and mass calibrate the detector
• Monitoring the analysis
• Acquiring data:
• Full Scan
• Full Scan with Fragmentation
• Processing data
• Reviewing data
• Printing reports

2.1.1 Calibrating and tuning the instrument

To calibrate and tune the instrument, use the Instrument Setup function on the Acquity RDa tab
to define the mass range and polarity settings, and then click Start.
for details on all the instrument's external connections.
August 20, 2021, 715005003 Ver. 04 (Previously released as Revision C)
Preparing the System for
Page 21
Fluidics System (Page