Table of Contents
Advertisement
6.
Troubleshooting
For questions not addressed here, please contact your local Promega Branch Office or Distributor. Contact information available at:
www.promega.com. E-mail: techserv@promega.com
Symptoms
Low or no reading
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Part# TM338
Page 18
Possible Causes and Comments
Use the Solid Standard to determine if the
instrument is functioning properly (with
QuantiFluor™-ST, Cat.# E6090 only; Section 3.K). If
the value of the Solid Standard deviates from the
previous reading by 5% or more, recalibrate the
instrument.
Check to ensure that all reagents and standards
are made to specification and not expired. Make
sure that dilutions were performed correctly.
Protect sample from light. Some fluorophores
are susceptible to photodegradation.
Make sure the correct optical channel is selected.
Make sure that the instrument is properly
calibrated before reading samples.
Ensure that the sample signal is within the linear
range of detection. Perform a dilution series if
necessary.
Ensure that the sample cuvette is appropriate.
Methacrylate cuvettes should be used for UV
applications.
Avoid sudden temperature fluctuations.
Ensure that sample volume is at least 2ml for
the 10 × 10mm cuvette or 50µl for the
QuantiFluor™-ST Minicell cuvette or 75µl
for the QuantiFluor™-P Minicell cuvette.
If you are using a QuantiFluor™-ST Minicell
Adapter, be sure that it is in the correct orientation.
If the BLUE channel is selected, insert the Minicell
Adapter in the Sample Compartment with the tab away
from you and the BLUE label facing you. If the UV
channel is selected, insert the Minicell Adapter in the
Sample Compartment with the tab close to you and the
UV label facing you.
Printed in USA.
Revised 1/11
Advertisement
Table of Contents
