Table of Contents
Advertisement
10. During reading the instrument screen will indicate "READING" or "WAITING". The result
will be displayed at the top, center of the screen when all counts are measured. At this point,
another sample can be read.
11. T
read next sample repeat steps 7–10.
O
3.I. QuantiFluor™ PCR tube Adapter Operation
The QuantiFluor™ PCR tube Adapter (Cat.# E6101) allows measurement of small
sample volumes in 0.5 ml PCR tubes, while still preserving the superior sensitivity
of the QuantiFluor™ Fluorometer. The PCR tube Adapter is multidirectional and
can be inserted into the Optical Kit sample compartment in any orientation.
Transfer the sample to a 0.5 ml PCR tube. The minimum volume required for
reproducible results is 100μl. Insert the PCR tube into the PCR tube Adapter, close
the lid, and measure the sample. Take care not to introduce air bubbles into the
sample.
3.J. Diagnostic Information
The Diagnostic screens show the number of points available and the current values for the calibration
and blank standards.
1.
Press the DIAG button to access the diagnostic screens.
2.
The first screen shows the number of available data points for internal data logging.
3.
Press the ENTER button to toggle to the RFU (Relative Fluorescence Unit) values from the
calibration blank and standard.
4.
Press the ESC button when finished to return to the HOME screen.
Note: If the instrument was calibrated with the highest concentration standard, and the RFU of
the standard is too close to that of the blank, the instrument will register a Standard<Blank Error
message.The RFU value of the Standard must be 3 times greater than the RFU value of the blank.
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Printed in USA.
Revised 1/11
QuantiFluor™
PCR tube Adapter.
Part# TM338
Page 13
Advertisement
Table of Contents
