Promega GloMax GM3000 Instructions For Use Manual
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Promega GloMax GM3000 Instructions For Use Manual

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O P E R A T I N G M A N U A L
GloMax
®
Instructions for Use of Product GM3000
Discover System
Revised 12/16
TM397

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Summary of Contents for Promega GloMax GM3000

  • Page 1 O P E R A T I N G M A N U A L GloMax Discover System ® Instructions for Use of Product GM3000 Revised 12/16 TM397...

  • Page 2: Table Of Contents

    6.B. Common Problems ......................78 6.C. Use of a USB Flash Drive ....................79 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 12/16...

  • Page 3: Introduction

    Figure 1. The GloMax Discover System. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16 www.promega.com...

  • Page 4: Specifications

    EU Declaration of Conformity for the product is available from Promega on request. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 5 AFC (Ex: 405nm, Em: 495–505nm) Detection Limit 2fmol fluorescein/200µl Linear Dynamic Range 6 logs (assay-dependent) Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16 www.promega.com...
  • Page 6 64GB Other Requirements Microsoft.NET Framework 4.0 (full version), Microsoft Excel 2013 Professional Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 12/16...

  • Page 7: C Product Components

    1 each Absorbance Filter Wheel containing 9 Absorbance filters (230, 260, 280, 320, 405, 450, 490, 560 and 600nm) Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...

  • Page 8: Precautions And Special Instructions

    UV light. faibles. Ne regardez pas directe- ment la lumière UV. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 12/16...
  • Page 9 • Do not leave plate in the instrument after protocol is completed. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16 www.promega.com...

  • Page 10: Environmental Requirements

    5–30°C, up to 75% humidity Operating Altitude Conditions less than 2,000 meters above sea level Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 12/16...

  • Page 11: Inspection And Unpacking

    The 96-well aperture, Fluorescence Filter Paddle and Luminescence Filter Paddle are preinstalled on the instru- ment. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 12 After removing the red Packing Bracket, remove the blue Packing Bracket Mount, and ensure the instru- ment is free of any packaging material parts. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 13: Setting Up The Glomax ® Discover System

    Connect the Instrument AC Power Adapter to the back of the instrument and to a power outlet. Note: We recommend connecting the instrument to an uninterrupted power supply (~500VA). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...

  • Page 14: Switching On The Glomax ® Discover Instrument

    Discover software. The GloMax Discover System is now ready to use. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 12/16...

  • Page 15: Shutting Down The Glomax ® Discover Instrument

    Select a protocol of interest or one of the Quick Reads protocols from the Protocols screen or from the yellow Quick Read options on the Home screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 16 Results button on the home screen. Results are exported to the location specified in Settings (Section 3.E, Export Folder). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 17: Glomax ® Discover User Interface Screens

    Exit Back Tools Door Help Figure 9. GloMax Discover ‘Home’ screen. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16 www.promega.com...

  • Page 18: Quick Read Screens

    Discover ‘Fluorescence Quick Read’ screen. On the plate map, green wells are ® selected. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 12/16...
  • Page 19 Start measurement. igure 12. GloMax Discover ‘Absorbance Quick Read’ screen. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16 www.promega.com...

  • Page 20: Protocol Screen

    Tap the Smart button, and select the protocol of interest. Follow the on-screen instructions. Insert the plate into the instrument. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 21 You will be prompted to name the protocol. You also may provide a description, if desired. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 22 Discover ‘New Protocol’ screen showing drag and drop feature to create custom ® protocols. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 12/16...
  • Page 23 The plate maps within each step of the protocol are then shaded and cannot be modified. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 ·...
  • Page 24 Fluorescence reads may select High Speed, which uses a 0.1 second integration time, or High Sensitivity, which uses a 0.3 second integration time. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 25 Figure 23. Ratio function for BRET and FRET reads. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 26 Note: The instrument is fan cooled, so the temperature cannot be set lower than the ambient temperature of your laboratory. Figure 25. The Heat Function. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 12/16...
  • Page 27 See Setting Up and Operating the Injector System (Section 4) for more details. Figure 27. Inject Function. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 28 Note: The instrument is not intended to be used as a long-term shaking incubator, and long-term shaking may damage the instrument. Figure 28. Shake Function. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 12/16...
  • Page 29 Figure 30. ‘Prompt’ screen with the Open Door option. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 30 Note: If adding an injector step inside a loop function, ensure that there is sufficient space in the well to accommodate multiple additions of reagent. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 31 All well-associated operations within the kinetic block must have the same well selection. The plate maps of these operations are not individually editable. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...

  • Page 32: Results' Screen

    Analyses. Creating Analysis Templates and performing Analyses are covered in Section 3.E, Analysis of Results. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 33 Export 1 or more results. Figure 35. GloMax Discover ‘Results’ screen. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16 www.promega.com...
  • Page 34 Figure 37. GloMax Discover ‘Results’ screen with heat map display. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 12/16...
  • Page 35 Microsoft Excel worksheet file. If creating a file for use with a LIMS, use the CSV file and import according to your LIMS software guidelines. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 36 Excel workbook. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 12/16...
  • Page 37 Data with time intervals. Each time value is based on ® when the first read was started (T = 0 seconds). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16 www.promega.com...

  • Page 38: Analysis Of Results

    Touch the Templates button to access existing or create new analysis templates. Touch the Analyses button to access saved data analyses. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 39 Each of these functionalities is described below in ‘Templates’ Screen Functions. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 40 Figure 45. The ‘Analyses’ screen. The ‘Analyses’ screen displays a list of all of the analyses that have been saved. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 41 If multiple analysis templates were selected, the file will have the name Templates. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 42 Touch Open to import the analysis template into the Templates list. The imported analysis template can now be selected when analyzing results files. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 43 Touch the Remove button on the bottom left side of the screen. Follow the prompts by selecting Yes to permanently remove the analysis template(s) or No to keep the analysis template(s). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 44 ‘Select Plate Type’ screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 45 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 46 Figure 50. Template with well designations assigned. In this figure unknown, known and blank well designations have been assigned across a plate. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 47 STAND name has been applied to the replicates in the standard curve, and BLANK has been assigned to the three Blank wells. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 48 (Figure 53). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 49 When multiple groups exist on a single plate, name text will be color coded to indicate individual groups (Figure 54). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 50 ‘Templates’ screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 51 On the left side of the ‘Protocols Execution Results’ screen, touch the Analyses button. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 52 Figure 55. Starting an analysis from the ‘Results’ screen. To start an analysis, touch the Analysis button at the bottom left side of the ‘Results’ screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 53 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 54 Run button to analyze that data set. Only one experimental data set can be analyzed at a time. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 55 Averages and standard deviations (assuming there are 3 or more replicates) for each replicate set will be indicated in the export file for the analysis. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 56 Specify a name for the template (using the same name will overwrite the existing template; a new name will create a new template). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 57 Use standard Excel functions to adjust the appearance of the graphed data. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16 www.promega.com...

  • Page 58: Settings' Screen

    Tools may be accessed from the Tools icon in the menu bar (shown below) or from Settings  Tools. Figure 60. Tools icon. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 59 There are two Apertures: 96-well and 384-well. Lower density plates (6-, 12-, 24- and 48-well plates) should use the 96-well aperture. The instrument comes with the 96-well Aperture preinstalled. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 60 Follow the on-screen steps. Figure 62. Aperture Instructions. Figure 63. Aperture Plate. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 12/16...
  • Page 61 Add the appropriate Filter Paddle of interest by inserting it in with the reverse process that you used to remove it. Tap Next to complete the reconfiguration. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16 www.promega.com...
  • Page 62 Figure 67. Filter configuration procedure (part 2). Figure 68. Filter configuration procedure (part 3). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 12/16...
  • Page 63 Custom excitation modules must be obtained by contacting Promega Technical Services (techserv@promega.com). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16 www.promega.com...
  • Page 64 Hold down the plate door, align the front access door and tighten the two screws at the bottom of the front access door to hold the door in place. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 65 Upon tapping the Export Logs button, the logs will export to the Tablet PC at the following location: C:\Exports\Logs Figure 75. ‘Export Logs’ screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16 www.promega.com...
  • Page 66 Select Save when finished. Figure 78. Settings  Administrator screen  Export Folder on the Settings menu. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 67 Select OK when finished. Figure 80. Settings  Administrator screen  Import Protocols. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 68 The context-dependent Help Screen can be accessed from any of the menu screens. Figure 82. Help Screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 69: Setting Up And Operating The Injector System (Optional)

    Figure 84. Positioning the Injector System on top of the GloMax Discover System. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16 www.promega.com...
  • Page 70 Figure 88. Installation of the inlet tube holder clip. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 12/16...
  • Page 71 Instrument and the Tablet PC. Power the Instrument back on, then turn on the Tablet Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...

  • Page 72: Operating The Injector System

    Note: The reagent container holder can accommodate four sizes of containers: a round flat-bottom bottle, a 50ml conical tube, a 15ml conical tube and a 15ml test tube. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 73 Once the injectors are primed, they are ready for use according to the programmed protocol. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 74 Note: Remove Waste Collection Tray when priming and flushing are complete. See Section 5.D for injector cleaning instructions. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 75: Cleaning And Maintenance

    Place an empty Waste Collection Tray on the Microplate Sample Tray. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...

  • Page 76: E Cleaning The Waste Collection Tray

    Discard the used injector tubing assembly. Twist the fitting of the replacement tubing into the correct port of the injector pump. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 77: Removing Or Replacing Stainless Steel Tubing

    ON the instrument. If the error persists, contact Promega Technical Services. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16 www.promega.com...
  • Page 78 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 12/16...

  • Page 79: Common Problems

    The luminescent output of the sample is saturating the detector. Use less sample. Use a black plate. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16 www.promega.com...

  • Page 80: Use Of A Usb Flash Drive

    Antivirus software will slow the performance of the Tablet PC and will affect the performance of the GloMax ® Instrument while it is measuring samples. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 12/16...
  • Page 81 Set the appropriate date and time for your current location using the calendar and clock setting icons. Tap the OK button to save the date and time settings. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 82 Figure 94. The ‘Windows Settings’ screen. The ‘Windows Settings’ screen provides access to multiple functions that can be used to configure options and settings for the Tablet PC. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 83 On the ‘Change date and time’ screen (Figure 96), use the drop-down boxes to select the appropriate date and time for your location. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 84 Swipe in from the right edge of the screen and select Search, then type in “lusrmgr.msc” and press Enter. The Local Users and Groups dialog should appear. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 85 Note: If the Local Users and Groups menu item is not visible, select the System Tools item to expand that menu heading. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 86 Close the ‘Computer Management’ screen. Figure 99. Adding users to the PromegaAdministrators group. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 12/16...
  • Page 87 PromegaUsers group. Close the ‘Computer Management’ screen. Figure 100. Adding users to the PromegaUsers group. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...
  • Page 88 ‘Local Users and Groups (Local)’ screen. Tap Local Users and Groups and double-tap Groups. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 89 Figure 103. ‘Instrument Name’ screen. Administrators can manually enter the instrument name from the ‘Instrument Name’ screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...

  • Page 90: Icons And Symbols

    Favorite. Mark to select a favorite protocol, which will then sort to the top of the page. Yellow favorite indicates selection. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 91: Warranty And Service

    (see Section 7.D). If the instrument has been exposed to any chemical, biological or radioactive hazards, contact Promega Technical Services for decontamination instructions before shipping. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16...

  • Page 92: Certificate Of Decontamination

    This instrument is certified by the undersigned to be free of radioactive contamination. Date: ____________________________________ Place: ____________________________________ Signature: _________________________________ Name (block capital letters): _______________________________________________________ Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 12/16...

  • Page 93: Related Products

    10ml G8091 ® Apo-ONE Homogeneous Caspase-3/7 Assay* G7792 ® *Additional Sizes Available. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16 www.promega.com...
  • Page 94 ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay* 1 plate E7110 *Additional Sizes Available Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 12/16...
  • Page 95 300 assays (384-well plate) V1501 GloSensor™ cAMP Reagent* 25mg E1290 *Additional Sizes Available. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 12/16 www.promega.com...

  • Page 96: Summary Of Changes

    Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date information on Promega products. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

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