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Summary of Contents for Promega Quantus Fluorometer
- Page 1 T e c h n i c a l M a n u a l Quantus™ Fluorometer Operating Manual INSTRUCTIONS FOR USE OF PRODUCT E6150. PRINTED IN USA. Revised 1/20 Part# TM396...
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Page 2: Table Of Contents
All technical literature is available on the Internet at: www.promega.com/protocols/ Please visit the web site to verify that you are using the most current version of this Technical Manual. Please contact Promega Technical Services if you have questions on use of this system. E-mail: techserv@promega.com 1. -
Page 3: Product Components
PCR tubes (Cat.# E4942 or Axygen Cat.# PCR-05-C, available from Fisher or VWR) Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM396 Printed in USA. -
Page 4: Specifications
Quantus™ Software) At least one USB port available Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM396 Revised 1/20... -
Page 5: Precautions And Special Instructions
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM396 Printed in USA. Page 4... - Page 6 Always disconnect the power before cleaning or performing routine maintenance. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM396...
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Page 7: Environmental Requirements
Select the appropriate outlet adapter for your wall outlet from the Outlet Adapter Kit provided. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM396 Printed in USA. -
Page 8: Setting Up The Quantus™ Software (Optional)
Expand “Ports”. “Quantus™ Serial Device” should be listed. Contact Promega Technical Services if you need assistance. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM396... -
Page 9: Operating The Quantus™ Fluorometer
3,800µl of nuclease-free water to prepare 4ml of 1X TE buffer. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM396 Printed in USA. - Page 10 Buffer, then use 10µl Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM396 Revised 1/20 Page 9...
- Page 11 These ratios have been predetermined as a quality check to help ensure a successful measurement. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM396 Printed in USA.
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Page 12: User-Defined Protocol For Other Dye Chemistries
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM396... -
Page 13: Quantitating Samples
1.0 to 1,000.0 range. 7. When the desired units are highlighted, press “Enter”. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM396 Printed in USA. -
Page 14: Tools
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM396... -
Page 15: Cleaning And Maintenance
Do not submerge the Quantus™ Fluorometer in water. Do not disassemble the unit. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM396 Printed in USA. -
Page 16: Troubleshooting
Troubleshooting For questions not addressed here, please contact your local Promega Branch Office or Distributor. Contact information available at: www.promega.com. E-mail: techserv@promega.com 5.A. Common Problems Symptoms Causes and Comments Low or no fluorescence reading Reagents were not prepared correctly. Ensure that reagents, blank and standard samples are prepared as described in Section 3.A. -
Page 17: Error Messages
The raw RFU values will still be displayed on the history screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM396 Printed in USA. -
Page 18: Appendix
Promega is not responsible for instrumentation returned without an authorization number. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM396... -
Page 19: Certificate Of Decontamination
Institution:____________________________________________________________ Signature: Name (block capital letters): Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM396 Printed in USA. Page 18 Revised 1/20... -
Page 20: Related Products
All prices and specifications are subject to change without prior notice. Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date information on Promega products. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 ·... - Page 21 Promega Corporation Promega Corporation • 2800 • 2800 W W oods Hollow Road oods Hollow Road Madison, WI 53711-5399 USA • Phone 608-274-4330...
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