Promega QuantiFluor E6090 Technical Manual page 23

8. Appendix
8.A. QuantiFluor™ Fluorometer
Workflow Chart
3.B Press ↑ or ↓ buttons to
adjust brightness of LCD
3.C Press A/B button to switch
between optical channels
X.X
UV
X.X
BLUE
X.X
GREEN (E6100 only)
3.F Press STD VAL button to
view value of standard
Cal Val: XX.X
<UP/DOWN>
3.J Press DIAG button to
view Diagnostics Screens
Logging Points
Left: XXX
Press ENTER button to toggle
between screens
RFU-BLK X.X
RFU-STD X.X
3.K After calibration, insert the Solid Standard
(QuantiFluor ST only) and press the READ button.
Confirm that the value does not deviate from
previously recorded value by more than 5% or more.
If it does, recalibrate the instrument. If it does not,
record the reading.
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Part# TM338
Page 22
Indicates which channel is
active (A or B)
3.G Press CAL button to
calibrate instrument
Calib
<ENT> to start
Press ENTER button to
move to next screen
Insert Blank
Then press <ENT>
Press ENTER button to
begin reading blank
Reading Blank
XX.X
Insert Cal Soln
then press <ENT>
Press ENTER button to
begin reading cal solution
BLUE
Calib Complete
press <ENT>
Press ENTER button to
accept calibration
If ENTER button is not
pressed within five seconds,
the following screen will
appear:
Abort Cal?
<↑> Yes <↓> No
QuantiFluor
Version XX xxxx
5-second warm up
X.XX.X
BLUE
HOME screen
BLUE
Press DATA button once
for Data Logging status
Press ESC button to return
to the HOME screen
Press DATA button twice
to download data
Press ESC button to return
to the HOME screen
Press DATA button three times
to erase data
4.9 If the message Standard<Blank, Recalibrate
appears, we recommend the standard
concentration be increased by 10X, or examine
the blank for high fluorescence background.
Note: The RFU of the standard (RFU-Std) must be
3 times greater than the RFU of the blank
(RFU-Blk) to calibrate the instrument.
Indicates Fluorescence Reading
and activity of IDL
continued on next page
4.A Press DATA button to
adjust data logging settings
Datalogger:
<DATA> to toggle
Status: Stop
<ENT> to toggle
Press ENTER button to toggle
between screens
Status: Logging
<ENT> to toggle
Download Data
5X <ENT> to start
Press ENTER button five times
to begin download
If no data are logged, the
following screen will appear:
Data Logger has
no valid data!
Erase DATA
5X <ENT> to start
Press ENTER button five times
to begin erasing data
Erase DATA
All data erased
Press ESC button to return
to the HOME screen
Printed in USA.
Revised 1/11