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3.H. Sample Analysis
The QuantiFluor™ Fluorometers are capable of measuring samples in two reading modes: Discrete or
Continuous. In Discrete Mode, the instrument measures and averages the fluorescence signal over a 5-
second interval. In Continuous Mode, the instrument measures and averages the fluorescence signal
over a user-defined interval for each sample measurement. The instrument measures the sample
repeatedly for a user-defined number of samples.
Discrete Mode
1.
Press the SET button to set the read mode.
2.
Press the UP ARROW or the DOWN ARROW button to toggle between Discrete and
Continuous Modes.
3.
Once the screen reads "Read Mode: Discrete", press the ENTER button.
4.
Insert the sample. Using the specified cuvette or the Minicell Adapter and Minicell Cuvette.
Note: The sample compartment lid must be closed before the instrument will read your sample.
5.
Press the READ button. The instrument will measure and average the fluorescence signal
over a 5-second interval. During this time the instrument screen will indicate "Reading". The
result will be displayed at the top center of the screen.
6.
Once the "Reading" message disappears, another sample can be read.
Continuous Mode
Note: For Continuous Mode, either the Internal Data Logging function must be enabled or the
instrument must be connected to an external PC or printer.
1.
Press the SET button to set the read mode.
2.
Press the UP ARROW or the DOWN ARROW button to toggle between Discrete and
Continuous Modes.
3.
Once the screen reads "Read Mode: Continuous", press the ENTER button.
4.
The screen will read "Continuous Meas Interval: 5 sec." Use the UP ARROW and DOWN
ARROW buttons to change the interval time.
5.
Press the ENTER button when your desired interval time is displayed. (5, 10, 30 or 60 seconds)
6.
The screen will display "Cont Meas Count". Use the UP ARROW and DOWN ARROW
buttons to select the number of samples you want to measure. Press the ENTER button.
7.
Press the READ button.
8.
The screen will display "CONT: count = XX <ENT> int = x sec". Press the ENTER button.
9.
The screen will display "Insert Sample Press <ENTER>". Insert your sample, close the compartment
lid, and press the ENTER button again.
Note: The sample compartment lid must be closed before the instrument will read your sample.
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Part# TM338
Page 12
Printed in USA.
Revised 1/11
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