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3.
Instrument Calibration and Operation
An instrument workflow chart is provided in Section 8.
3.A. Instrument Power Up and Shut Down
To turn on the QuantiFluor™ Fluorometer, press the ON/OFF button. The instrument takes five seconds
to warm up. After the warm-up, the QuantiFluor™ Fluorometer is ready for use. Pressing the ON/OFF
button again will turn off the unit.
QuantiFluor™-P units only: If left idle for three minutes, the unit will automatically turn off to save
battery power.
A set of new batteries can last over 1,000 sample readings. If the batteries have low power or are not
positioned properly, the following warning message will appear: "Batt Level < 20% !! Caution !!"
QuantiFluor™-ST units only: After switching off the unit, unplug the AC Adapter from the wall outlet.
3.B. LCD
The QuantiFluor™ Fluorometer has a backlit, adjustable-brightness LCD (Liquid Crystal Display) for
easier viewing. The contrast may change with temperature and may get lighter as the unit gets colder.
While on the HOME screen, adjust the brightness of the LCD using the UP ARROW and DOWN
ARROW buttons.
3.C. Detection Channel Configuration
The QuantiFluor™ Fluorometers have two fluorescence detection channels. Identify the configuration by
looking at the label on the back side of the QuantiFluor™ Fluorometer. See the Optical Specifications
table in Section 1.D to confirm the correct configuration for your application.
Select the appropriate channel by pressing the A/B button to toggle between the two channels. The display
will show a label in the lower left corner of the HOME screen to identify which channel is activated.
QuantiFluor™-ST Fluorometer (Cat.# E6090)
Channel A
Channel B
QuantiFluor™-P Fluorometer (UV, Blue; Cat.# E6105)
Channel A
Channel B
QuantiFluor™-P Fluorometer (Green, Blue; Cat.# E6100)
Channel A
Channel B
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Printed in USA.
Revised 1/11
UV (365nm
, 440–470nm
Ex
Em
Blue (460nm
, 515–575nm
Ex
Em
UV (365 nm
, 440–470nm
Ex
Em
Blue (460 nm
, 515–575nm
Ex
Green (525nm
, >570nm
Ex
Em
Blue (460nm
, 515–575nm
Ex
Em
),
)
),
)
Em
)
)
Part# TM338
Page 9
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