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QuantiFluor™ Handheld Fluorometers
Operating Instructions
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Please visit the web site to verify that you are using the most current version of this Technical Manual.
Please contact Promega Technical Services if you have questions on the use of this product. Email: techserv@promega.com
1.
Introduction...................................................................................................................................................................2
Description...........................................................................................................................................................2
Product Components .........................................................................................................................................2
QuantiFluor™ Handheld Fluorometers General Specifications .................................................................4
QuantiFluor™ Handheld Fluorometers Optical Specifications ..................................................................4
Inspection.............................................................................................................................................................4
Precautions and Special Instructions...............................................................................................................5
Environmental Requirements ...........................................................................................................................7
2.
Setting up the QuantiFluor™ Handheld Fluorometers .......................................................................................7
3.
Instrument Calibration and Operation....................................................................................................................9
Instrument Power Up and Shut Down ...........................................................................................................9
LCD .......................................................................................................................................................................9
Detection Channel Configuration ....................................................................................................................9
Calibration Overview.......................................................................................................................................10
Calibration .........................................................................................................................................................10
Setting Calibration Standard Value ...............................................................................................................11
Performing the Calibration .............................................................................................................................11
Sample Analysis................................................................................................................................................12
I.
QuantiFluor™ PCR Tube Adapter Operation .............................................................................................13
Diagnostic Information ....................................................................................................................................13
Solid Standard ...................................................................................................................................................14
4.
Internal Data Logging (IDL) ....................................................................................................................................15
Activating IDL...................................................................................................................................................15
Downloading Data to a Computer ................................................................................................................15
Erasing Data from the QuantiFluor™ Instrument ......................................................................................15
5.
General Considerations ............................................................................................................................................16
Handling Samples.............................................................................................................................................16
Linear Range and Quenching .........................................................................................................................16
Temperature ......................................................................................................................................................16
Sample Position.................................................................................................................................................17
Data Quality ......................................................................................................................................................17
Periodic Cleaning and Maintenance..............................................................................................................17
6.
Troubleshooting .........................................................................................................................................................18
7.
Internal Data Logging (IDL) ....................................................................................................................................20
Shipping Checklist............................................................................................................................................20
Hardware Requirements for IDL ...................................................................................................................20
Installing IDL Software....................................................................................................................................20
Connecting the QuantiFluor™ Fluorometer ................................................................................................20
Troubleshooting Internal Data Logging........................................................................................................21
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Printed in USA.
Revised 1/11
Part# TM338
Page 1
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