Related Manuals for Promega QuantiFluor E6090
Summary of Contents for Promega QuantiFluor E6090
- Page 1 T e c h n i c a l M a n u a l QuantiFluor™ Handheld Fluorometers Operating Instructions INSTRUCTIONS FOR USE OF PRODUCTS E6090, E6105 AND E6100. PRINTED IN USA, Revised 1/11 Part# TM338...
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Page 2: Table Of Contents
Connecting the QuantiFluor™ Fluorometer ....................20 Troubleshooting Internal Data Logging......................21 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM338... -
Page 3: Introduction
QuantiFluor™ PCR Tube Adapter 1 each E6101 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM338 Printed in USA. Page 2... - Page 4 See the Related Products Section at the end of this Manual for additional accessories. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA.
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Page 5: Quantifluor™ Handheld Fluorometers General Specifications
Spreadsheet Interface Software (1 CD) • CD containing Technical Manual Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM338 Printed in USA. -
Page 6: Precautions And Special Instructions
électrique lorsqu'il n'est pas utilisé (Cat.# E6090) power outlet when not in use (Cat.# E6090). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. - Page 7 Avoid having any air bubbles in your sample because these may significantly affect the fluorescence reading. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM338 Printed in USA.
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Page 8: Environmental Requirements
To adjust the brightness of the LCD, press the UP ARROW or DOWN ARROW buttons. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. - Page 9 Figure 4. Front Panel of the QuantiFluor™-ST Handheld Fluorometer. The control panel of the QuantiFluor™-P instrument is identical. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM338 Printed in USA.
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Page 10: Instrument Calibration And Operation
, >570nm Channel B Blue (460nm , 515–575nm Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM338 Revised 1/11... -
Page 11: Calibration Overview
-ST only) is useful for checking the reading stability over time. Cuvette orientation and cleanliness also can affect accuracy. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM338 Printed in USA. -
Page 12: Setting Calibration Standard Value
HOME screen, and the instrument will default to the previous calibration. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. -
Page 13: Sample Analysis
Note: The sample compartment lid must be closed before the instrument will read your sample. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM338 Printed in USA. -
Page 14: Quantifluor™ Pcr Tube Adapter Operation
RFU value of the Standard must be 3 times greater than the RFU value of the blank. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. -
Page 15: Solid Standard
Note: If you recalibrate the channel, then a new measurement for the Solid Standard must be taken and stored for future reference. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM338 Printed in USA. -
Page 16: Internal Data Logging (Idl)
"Erase Data All data erased." Press the ESC button to return to the HOME screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. -
Page 17: General Considerations
For greatest accuracy, read the blank, standard and samples at the same temperature. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM338 Printed in USA. -
Page 18: Sample Position
Always power off the instrument before cleaning. Be sure to unplug the QuantiFluor™- Fluorometer before cleaning. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. -
Page 19: Troubleshooting
Sample Compartment with the tab close to you and the UV label facing you. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM338 Printed in USA. - Page 20 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM338...
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Page 21: Internal Data Logging (Idl)
Be sure to save these data before closing the software. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM338 Printed in USA. -
Page 22: Troubleshooting Internal Data Logging
IDL not activated. Refer to Section 4 for instructions. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM338... - Page 23 HOME screen (RFU-Blk) to calibrate the instrument. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM338 Printed in USA.
- Page 24 WAITING #X XX.X XXX.X BLUE Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM338 Revised 1/11 Page 23...
- Page 25 Contact your local Promega representative for disposal of the instrument. Please follow your institutional guidelines to handle disposal of accessories. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM338 Printed in USA.
- Page 26 Date:________________________ Place:________________________________________________________________________________________ Signature: Name (block capital letters): Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM338 Revised 1/11...
- Page 27 Repair service will be billed at a flat rate. Your invoice will include freight charges. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM338 Printed in USA.
- Page 28 Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date information on Promega products. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA.