Troubleshooting; Optical - Accu-Scope EXI-600 Manual

Inverted research microscope
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Under certain conditions, performance of this unit may be adversely affected by factors other than
defects. If a problem occurs, please review the following list and take remedial action as needed. If you
cannot solve the problem after checking the entire list, please contact your local dealer for assistance.

10.1. OPTICAL

Problem
Illumination is turned on, but the
field of view is still dark.
The field of view has a shadow
or is unevenly illuminated.
Dirt or dust in the field of view.
A ghost image of the sample is
observed.
Poor image quality.
Low resolution
Low contrast
ACCU-SCOPE
®
73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com
EXI-600 INVERTED MICROSCOPE SERIES

10. TROUBLESHOOTING

Cause
The LED bulb is burned out.
Illumination brightness is
adjusted too low.
There are too many filters in the
light path absorbing light.
The objective nosepiece is not
fully in position.
Filter is not fully inserted or
removed.
Condenser turret position is not
fully engaged.
Dirt or dust in the light path, e.g.,
on the eyepiece, condenser,
objective, collector lens or
specimen
Condenser aperture diaphragm
is too closed.
Microscope optical and
illumination components are not
properly or optimally aligned.
The objective nosepiece is not
in the correct position.
Condenser aperture diaphragm
is too small or too big for
brightfield observation.
Lenses or sample in the optical
path (condenser, objective,
eyepiece or culture dish) is dirty.
The thickness of culture dish
bottom is over 1.2mm.
A brightfield objective is being
used for phase contrast
observation.
The phase contrast annulus
(ring) does not match the
Corrective Measure
Replace with new 5W LED
lamp.
Turn up light intensity. Check
LCD panel for brightness level.
Remove unnecessary filters
from the light path (e.g.,
polarizers).
Turn the nosepiece so that the
detent is engaged in a position.
Push the filter all the way in, or
remove it from the light path.
Rotate the condenser turret until
it sets completely into a position
as felt by the detent.
Carefully clean the optics and
specimen slide/vessel. Observe
again and repeat until view is
free of dirt.
Open the condenser aperture
diaphragm.
Perform Köhler illumination.
Turn the nosepiece until the
correct objective is in position.
Adjust the condenser aperture
diaphragm according to Köhler
illumination.
Carefully clean the optics and
specimen slide/vessel.
Use sample slides and vessels
with thickness ≤1.2mm.
Change to a phase contrast
objective.
Ensure the correct phase
contrast position on the
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