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6.2.2. Reflected Light Methods
6.2.2.1. Fluorescence observation – Instrument configuration
Component
Transmitted illumination shutter
Reflected light shutter
Reflected light field diaphragm
Reflected light aperture diaphragm
Neutral density filter slider
Fluorescence light source
Upper light path selector
Lower light path selector
Condenser turret position
Magnification changer
Fluorescence cube turret (if
installed)
Objective(s)
1. Confirm that fluorescence excitation illumination is coming from the objective.
2. Place a specimen on the stage.
3. Focus on the specimen using the focus knobs.
4. Change fluorescence cube turret position to a different filter cube and observe again.
5. If desired, change side port light path selector or trinocular tube upper light path selector to a
camera port for imaging.
ACCU-SCOPE
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EXI-600 INVERTED MICROSCOPE SERIES
Off (closed), no light is coming out of the bottom of the
condenser
On (open)
Fully open
Fully open
Open position
On and shutter open
Any position. Condenser is not being used during
fluorescence observation. It is recommend to tilt the light pillar
to the back position to avoid reflection and autofluorescence of
the transmitted LED light source.
Choose an appropriate filter cube for the specimen
Any, as provide appropriate magnification of the specimen
Position
1X
37
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