Inline Pressure Relief Valve Kit (G4212-68001); Application Information; Special Information Of 60 Mm Cartridge Flow Cell - Agilent Technologies InfinityLab LC Series User Manual

5 Optimizing the Detector
Optimization Overview

Inline Pressure Relief Valve Kit (G4212-68001)

When several detectors are installed in a system the connecting capillary and
fittings between the detectors must be carefully chosen to keep
chromatographic influence on peak shape small. On the other hand narrow bore
connection capillaries generate a significant pressure drop dependent on flow
rate and solvent properties.
The pressure relief valve is designed to protect the Max-Light Cartridge Flow Cell.
Agilent Technologies strongly recommend installing the pressure relief valve at
the outlet of the detector as soon as a second detector is installed like in LC/MS
applications.
The pressure relief valve is a low internal volume check valve. The dead volume is
smaller than 100 nL delay volume (inlet to outlet). The ball of the check valve is
spring loaded and adjusted to open at typically 80 bar. On overpressure (typically
above 80 bar) it releases the pressure to waste.

Application Information

For the analysis and characterization of proteins and large biomolecules, and
applications with mobile phases of a pH above 12.5 it is recommended to use an
Agilent Diode Array Detector G1315C/D and G7115A (or Multiple Wavelength
Detector G1365C/D and G7165A) and the respective bio-inert flow cell.

Special Information of 60 mm Cartridge Flow Cell

Application Information
The geometrical volume of the 60 mm cell is 6 times larger than the 10 mm cell.
However, the chromatographic relevant dispersion volume, the square roots of
variances, accounting for cell specific geometrical volume shape and fluidic flow
pattern, have been determined as V = 4 µL and V = 1 µL in for the 10 mm cell.
Due to the larger dispersion volume, the 60 mm cell is primarily designed for
4.6 mm column applications to achieve highest sensitivity with no additional
peak broadening. However, if sensitivity is important the 60 mm cell will also be
advantageous in case of smaller columns (3 mm, 2.1 mm) but depending on the
chromatographic system and method additional peak broadening might occur.
Agilent InfinityLab LC Series Diode Array Detectors User Manual 81