Post-Run Uv Light Treatment - Promega Maxwell 16 MDx Operating Manual

5.C. Performing a Sample Purification Run (continued)
If a calibration error occurs, note the Error Code Number displayed on the touch screen and refer to the Trouble-
shooting section (Section 9) for more information. If you are in the middle of a run, you can choose to continue
the purification procedure or to abandon the run. Continuing the run will not damage the Maxwell
Instrument but may result in suboptimal recovery of nucleic acid from the samples. Refer to the Troubleshooting
section for a detailed list of error codes. To cancel the run, press the Run/Stop button on the instrument keypad to
stop the run, and follow the on screen instructions.
At the end of the run, the touch screen will display a message that the method has ended. The export of run data
occurs before the samples are removed from the instrument (see Section 6).
After the run is complete, open the instrument door and check that all of the plungers have been ejected
from the magnetic rod assembly. If a plunger is still attached, push it down gently by hand to remove it.
Select the Run/Stop button to extend the platform. Remove the elution tubes and place them in the
Magnetic Elution Tube Rack (for SEV protocols) to remove any residual particles. The eluted sample can
then be transferred to a storage tube.
Important. To avoid particle transfer after SEV runs, use a pipette tip to aspirate samples away from the
!
captured particles on the side of the blue elution tube.
5.D. Post-Run UV Light Treatment
If you have configured your instrument to perform a UV light treatment at the end of each run, that
treatment will start when the door is closed. Ensure that the samples are removed before closing the
door and starting the UV light treatment to avoid damage to the nucleic acid. You may cancel the UV
treatment at any time.
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TM320 · Revised 4/15
Figure 28. End screen.
16 MDx
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