Procedure For Changing The Cover Slip Mountant; Xylene-Based Coverslipping Mountant -> Xylene-Based Coverslipping Mountant; Xylene Substitute-Based Mountant -> Xylene-Based Mountant; Xylene-Based Mountant -> Xylene Substitute-Based Mountant - Leica CV5030 Operating Manual

6.3

Procedure for changing the cover slip mountant

6.3.1 Xylene-based coverslipping mountant —> xylene-based coverslipping mountant
6.3.2 Xylene substitute-based mountant —> xylene-based mountant
6.3.3 Xylene-based mountant —> xylene substitute-based mountant
Leica CV5030 – Robotic Coverslipper
Due to the high pressure when rinsing with xylene, it is necessary
to wear safety goggles when changing the cover slip mountant!
• Remove the mountant bottle.
• Rinse with 200 ml of xylene.
• Insert the mountant bottle with new mountant (do not forget priming,
hoses must be filled completely).
If the sequence of individual substances is not followed, milky/
cloudy clumps in the hose and dispenser system may result. It may
become completely clogged, causing the dispenser function to
fail. In this case, the hoses must be replaced completely.
• Remove the mountant bottle.
• Rinse with 200 ml of xylene substitute.
• Rinse with 200 ml 100% ethanol.
• Rinse with 200 ml of xylene.
• Insert the mountant bottle with new mountant (do not forget priming,
hoses must be filled completely).
• Remove the mountant bottle.
• Rinse the hose system with 200 ml of xylene.
• Rinse the hose system with 200 ml of 100% ethanol.
• Rinse the hose system with the 200 ml of xylene substitute.
• Insert the mountant bottle with new substitute-compatible mountant
(do not forget to prime, hoses must be filled completely).
Likewise, all parts filled with xylene must be converted to the
substitute (= loading bath, glass vial for parking the nozzle, nozzle
cleaner)!
6. Maintenance and Cleaning
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