Procedure For Changing The Cover Slip Mountant; Changing From One Xylene-Based Coverslip Mountant To Another; Changing From A Xylene Substitute Coverslip Mountant To A Xylene-Based Mountant; Changing From A Xylene-Based Coverslip Mountant To A Xylene Substitute - Leica CV5030 Instructions For Use Manual

7. Cleaning and Maintenance
7.8

Procedure for changing the cover slip mountant

Before switching the mountant, switch
off the instrument and disconnect it from
the power supply! Remove the loading
bath and specimen slide holders from
the instrument before switching it off.
Due to the high pressure when rinsing
with solvent, it is necessary to wear
gloves, safety goggles and appropri-
ate protective clothing when changing
the cover slip mountant! Select an ad-
equately sized container to catch the
rinsing residue.
7.8.1 Changing from one xylene-based
coverslip mountant to another
• Remove the mountant bottle.
• Insert a second glass bottle with 150 ml of
xylene and rinse/prime it (see
• Insert a mountant bottle with the new moun-
tant.
• Run a prime cycle. Hold down the PRIME
button until the hose is completely free of air
bubbles and filled with coverslip mountant.
7.8.2 Changing from a xylene substitute
coverslip mountant to a xylene-based
mountant
The sequence of individual substanc-
es has to be followed to avoid milky/
cloudy clumping in the hose and the
dispenser group.
88
• Remove the mountant bottle.
• Insert a second glass bottle with 150 ml of
• Empty the second glass bottle and fill it with 150 ml
• Empty the second glass bottle and fill it with
• Insert a mountant bottle with the new mountant.
• Run a prime cycle. Hold down the PRIME
7.8.3 Changing from a xylene-based
Chap. 7.3 B). • Remove the mountant bottle.
• Insert a second glass bottle with 150 ml of
• Empty the second glass bottle and fill it with
• Empty the second glass bottle and fill it with
• Insert a mountant bottle with the new, substi-
• Run a prime cycle. Hold down the PRIME
xylene substitute and rinse/prime it (see
Chap. 7.3 B).
of 100 % ethanol before inserting it and rinsing.
150 ml of xylene before inserting it and rinsing/
priming.
button until the hose is completely free of air
bubbles and filled with coverslip mountant.
coverslip mountant to a xylene
substitute
xylene and rinse/prime it (see
150 ml of 100 % ethanol before inserting it and
rinsing.
150 ml of xylene substitute before inserting it
and rinsing/priming.
tute-compatible mountant.
button until the hose is completely free of air
bubbles and filled with coverslip mountant.
All parts that were filled with xylene
must be converted to the substitute
(= loading bath, glass vial, dispenser
needle cleaner)!
Instructions for Use V 3.3 RevK – 04/2019
Chap. 7.3 B).