3DHISTECH Pannoramic 250 FLASH User Manual page 47

Pannoramic 250 FLASH 1.14 SP1 User's Guide
Use threshold
Brightfield preview – a normal type of illumination in the microscope for scanning the slide.
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Darkfield preview – the preview image is created with transmitted Darkfield illumination.
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This option is recommended for you to choose in case the sample is not correctly stained (for
example, the specimen is lacking contrast) when using Brightfield illumination. The image is
created in 2-3 minutes.
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March 30, 2011
Figure 27 – Preview tab
Scan auto thresholded specimen – This automatically detects the specimen in the
preview image.
Scan thresholded specimen – This sets the sensitivity of the specimen detection. To
set the desired threshold value manually, change the setting in the Specimen
threshold level selection box in the range of 0-255. The default value is 35.
Smaller values result in a more sensitive scanning, meaning that more areas will be
digitized, however, this may also include specks or other imperfections of the slide.
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