Setting The Chromatographic Conditions - Agilent Technologies 1260 Infinity User Manual

4
Using the Fluorescence Detector
Getting Started and Checkout

Setting the Chromatographic Conditions

1 Set up the system with the following chromatographic conditions and wait
Table 15
Mobile phases
Column
Sample
Flow rate
Compressibility A (water)
Compressibility B (Acetonitrile)
Stroke A and B
Stop time
Injection volume
Oven temperature (1200)
FLD Excitations/Emission Wavelength
FLD PMT Gain
FLD Response time
2 Set the FLD setpoints according to
72
until the baseline gets stable.
Chromatographic Conditions
A = water = 35 %
B = Acetonitrile = 65 %
OSD-Hypersil column, 125 mm x 4 mm i.d. with 5
µm particles
Isocratic standard sample, 1:10 diluted in
methanol
1.5 ml/min
46
115
auto
4 min
5 µl
30 °C
EX = 246 nm, EM = 317 nm
PMT = 10
4 s
Figure 27 on page 73.
Agilent 1260 FLD User Manual