Agilent Technologies 1260 Infinity User Manual page 25

1
Introduction to the Fluorescence Detector
Analytical Information From Primary Data
Figure 14 LAMP: Frequency of Flash, Fluorescence, and Phosphorescence
You can improve the signal-to-noise characteristics by disabling the "economy"
mode.
Disabling the "economy" mode will shorten the lifetime of the lamp significantly. Consider
N O T E
lifetime saving by switching off the lamp after the run is completed.
The data resolution is 20 bit at a response time of 4 s (default, which is
equivalent to a time constant of 1.8 s and appropriate for standard
chromatographical conditions). Weak signals may cause errors in
quantification because of insufficient resolution. Check your proposed
PMTGAIN. If it is significantly distant from your setting, change your method
or check the purity of your solvent.
You can amplify the signal using PMTGAIN. Depending on the PMTGAIN you
have set, a multiple of electrons is generated for every photon falling on the
photomultiplier. You can quantify large and small peaks in the same
chromatogram by adding PMTGAIN changes during the run into a timetable.
Agilent 1260 FLD User Manual 25