Fld Scaling Range And Operating Conditions - Agilent Technologies 1260 Infinity User Manual

Fluorescence detector
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5
Optimizing the Detector
Finding the Best Signal Amplification

FLD Scaling Range and Operating Conditions

FLD Scaling Range and Operating Conditions
When using different FLD
• The signal height of individual G1321 FLD modules may exceed the
• Different G1321 FLD modules show different signal heights with identical
Both scaling issues can be resolved. Refer to
PMT-Gain-Level"
Optimize the PMT-Gain-Level
Start the PMT-Gain-Test with your operating conditions (used method
parameter, EX-/EM-wavelength, solvent, flow rate, ...). The resulting PMT
value will give you the best signal to noise performance with the maximum
usable signal range for this method and this specific instrument. For another
FLD this PMT level may vary (based on the individual PMT-Gain-Test).
The figure below demonstrates the impact of changing the PMT Gain.
110
recommended signal range 0 – 100 LU. Under certain circumstances this
could lead to clipped peaks.
methods. This is not a problem in general but could be inconvenient when
operating more than one G1321 FLD in the lab.
on page 110.
"Optimize the
Agilent 1260 FLD User Manual

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