Table 3-1. Acceleration/Deceleration Times (In Minutes:seconds) - Beckman Coulter Allegra 21 Series Instruction Manual

Setting Acceleration Rate
➠
NOTE
For runs at other than room temperature, refrigerate
or warm the rotor beforehand for fast equilibration.
For low-temperature runs, precool the centrifuge by
running a 30-minute cycle at the desired temperature
(with a precooled rotor installed) with the speed set
at about 2000 rpm.
The [ ] key is used to select acceleration rates that will protect deli-
ACCEL
cate gradients. When the [
flashes, indicating that one of the 10 preset acceleration rates can be
entered with the cursor keys. The selected rate will depend on the type
of run you are performing. For pelleting runs, where sample mixing is not
a concern, maximum acceleration can be used. However, if delicate
gradients are being run, a lower setting may be needed. Acceleration rates
are listed in Table 3-1.

Table 3-1. Acceleration/Deceleration Times (in minutes:seconds).

Times are approximate; actual times will vary depending on rotor load.
Refrigerated Models
Horizontal
Rotors
(5 500 rpm)*
Curve Accel Decel
9 0:54 0:40
8 0:54 0:29
7 0:56 1:03
6 1:40 1:39
5 2:30 2:26
4 3:18 3:11
3 4:54 4:48
2 9:41 9:35
1 14:40 14:11
0 14:40 14:11
*Maximum speed for the S2096 microtiter rotor is 3000 rpm; acceleration and deceleration times will decrease
accordingly.
] key is pressed, the ACC/DEC display
ACCEL
Fixed Angle Horizontal
Rotors Rotors
(15 300 rpm) (4 500 rpm)*
Accel Decel Accel
0:47 1:04 0:25
1:20 1:17 0:27
2:29 2:28 0:29
4:55 4:52 0:30
7:39 7:17 1:04
10:02 9:42 1:08
15:16 14:33 2:13
29:07 30:56 4:31
45:56 46:16 12:03
61:01 46:16 12:03
Nonrefrigerated Models
Fixed Angle
Rotors
(14 500 rpm)
Decel Accel Decel
0:23 0:44 0:47
0:24 1:06 1:09
0:26 2:03 2:12
0:30 4:11 4:12
1:07 6:13 6:14
1:11 8:07 8:05
2:18 11:12 11:26
4:34 23:17 23:20
12:17 40:00 40:16
12:17 40:00 40:16
Operation
3-9